公开(公告)号：US20190054122A1

公开(公告)日：2019-02-21

申请号：US16180867

申请日：2018-11-05

申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  INTIMA BIOSCIENCE, INC. ,  The United States of America, as represented by the Secretary, Department of Health and Human

发明人： Branden Moriarity ,  Beau Webber ,  Modassir Choudhry ,  Steven A. Rosenberg ,  Douglas C. Palmer ,  Nicholas P. Restifo

IPC分类号： A61K35/17 ,  C12N15/90 ,  C07K14/47 ,  C07K14/705 ,  C07K14/725 ,  C12N9/96 ,  C12N9/22 ,  C12N5/0783 ,  C07K14/715 ,  C12N15/113 ,  C12N15/87

摘要： Genetically modified compositions, such as non-viral vectors and T cells, for treating cancer are disclosed. Also disclosed are the methods of making and using the genetically modified compositions in treating cancer.

公开(公告)号：US20220088074A1

公开(公告)日：2022-03-24

申请号：US17429452

申请日：2020-02-21

申请人： Regents of the University of Minnesota

发明人： Beau Webber ,  Branden Moriarity ,  Emily Joy Pomeroy ,  Patricia Nicole Claudio Vazquez

IPC分类号： A61K35/17 ,  C12N5/0783 ,  A61P35/00

摘要： Provided herein are genome-edited γδ T cells that exhibit an increased capacity to kill cancer cells, methods of producing genome-edited γδ T cells, and methods of treating or preventing a condition by administering genome-edited γδ T cells to a subject in need thereof.

公开(公告)号：US20240042029A1

公开(公告)日：2024-02-08

申请号：US18266220

申请日：2021-12-08

申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA

发明人： Branden Moriarity ,  Beau Webber ,  Kenta Yamamoto ,  Joseph Skeate

IPC分类号： A61K39/00 ,  C12N5/0783 ,  C12N15/90 ,  C12N15/11 ,  C12N9/22

CPC分类号： A61K39/4631 ,  C12N5/0646 ,  C12N15/907 ,  C12N15/11 ,  C12N9/22 ,  A61K39/4611 ,  A61K39/4613 ,  C12N2502/99 ,  C12N2310/20 ,  C12N2800/80

摘要： Provided herein are methods and compositions for targeted delivery of cargo molecules, including, for example, gene editing reagents, RNA binding proteins, therapeutic agents, or other cargo via trogocytosis. In particular, provided herein are genetically modified donor cells as well as methods of using such genetically modified donor cells to deliver cargo of interest fused to a transmembrane receptor to a specific acceptor target cell.

公开(公告)号：US20220002717A1

公开(公告)日：2022-01-06

申请号：US17290968

申请日：2019-11-08

申请人： Regents of the University of Minnesota

发明人： Branden Moriarity ,  Mitchell Kluesner ,  Beau Webber

IPC分类号： C12N15/11 ,  C12N9/22 ,  C12N9/24 ,  C12N9/78 ,  C12N9/06

摘要： Provided herein are methods and compositions for highly precise base editing and single strand nicking. In particular, provided herein are methods for producing a genetically modified cell where the methods employ a universal, highly precise base editor or staggered Cas9 editor for precise base editing with minimal off-target or bystander effects.

公开(公告)号：US20210040507A1

公开(公告)日：2021-02-11

申请号：US16979722

申请日：2019-03-13

申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA

发明人： Branden Moriarity ,  Beau Webber ,  Cara-Lin Lonetree ,  Miechaleen Diers ,  Mitchell Kluesner ,  Walker Lahr ,  Emily Joy Pomeroy

IPC分类号： C12N15/90 ,  C12N5/0783

摘要： Provided herein are methods and systems for targeted gene disruption (knock-out, missense mutation) and targeted gene knock-in in mammalian cells using base editors and guide RNAs (gRNAs) designed to target splice acceptor-splice donor sites. Also provided herein are universally acceptable genetically engineered cells comprising targeted disruptions in immunotherapy-related genes and comprising a CAR/TCR for therapeutic applications.