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公开(公告)号：US5856110A
公开(公告)日：1999-01-05
申请号：US440401
申请日：1995-05-12
IPC分类号： A61K38/00 , C07K14/475 , G01N33/53
CPC分类号： C07K14/4756 , A61K38/00
摘要： Novel 2 polypeptides with binding affinity for the p185.sup.HER2 receptor, designated heregulin 2-.alpha. and heregulin 2-.beta., have been identified and purified from human tissue. The cDNA encoding the novel heregulin 2-.alpha. has been isolated from human tissue and sequenced. Provided herein is nucleic acid sequence of the heregulin 2-.alpha. useful in the production of heregulin 2-.alpha. by recombinant means. Further provided an amino acid sequence of heregulin 2-.alpha. and heregulin 2-.beta.. Heregulins and their antibodies are useful as therapeutic agents and in diagnostic methods.
摘要翻译： 已经从人体组织中鉴定并纯化具有与p185HER2受体结合亲和力的新型2多肽，称为asgulin 2-α和asgulin 2-β。 已经从人组织中分离出编码新型拟胰岛素2-α的cDNA并测序。 本文提供了可用于通过重组方法产生拟胰岛素2-α的asgulin 2-α的核酸序列。 进一步提供了asgulin 2-α和asgulin 2-beta的氨基酸序列。 本土及其抗体可用作治疗剂和诊断方法。
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公开(公告)号：US5641869A
公开(公告)日：1997-06-24
申请号：US456201
申请日：1995-05-31
IPC分类号： C07K7/08 , A61K38/00 , C07K20060101 , C07K1/18 , C07K14/00 , C07K14/47 , C07K14/475 , C07K16/00 , C07K16/18 , C12N20060101 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/12 , C12P21/02 , C12P21/08 , C12Q1/68 , C12R1/19 , C12R1/91 , C07K1/14 , C07K1/20 , C07K1/22
CPC分类号： C07K14/4756 , A61K38/00
摘要： A novel polypeptide with binding affinity for the p185.sup.HER2 receptor, designated heregulin-.alpha., has been identified and purified from cultured human cells. DNA sequences encoding additional heregulin polypeptides, designated heregulin-.alpha., heregulin-.beta.1, heregulin-.beta.2, heregulin-.beta.2-like, and heregulin-.beta.3, have been isolated, sequenced and expressed. Provided herein are nucleic acid sequences encoding the amino acid sequences of heregulins useful in the production of heregulins by recombinant means. Further provided are the amino acid sequences of heregulins and purification methods therefor. Heregulins and their antibodies are useful as therapeutic agents and in diagnostic methods.
摘要翻译： 已经鉴定并从培养的人细胞中纯化了对p185HER2受体具有结合亲和力的新型多肽，命名为asgulin-α。 已经分离，测序和表达编码附加的调节蛋白多肽的DNA序列，称为asgulin-α，asgulin-β1，heregulin-β2，heregulin-β2样和asgulin-β3。 本文提供了通过重组方法编码可用于生产本身的古菌素的氨基酸序列的核酸序列。 此外还提供了本文的氨基酸序列及其纯化方法。 本土及其抗体可用作治疗剂和诊断方法。
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公开(公告)号：US5464756A
公开(公告)日：1995-11-07
申请号：US908766
申请日：1992-07-01
IPC分类号： A61K38/00 , A61P13/02 , A61P15/00 , C07H21/04 , C07K1/113 , C07K1/12 , C07K14/00 , C07K14/575 , C07K14/64 , C12N1/21 , C12N15/09 , C12N15/12 , C12N15/16 , C12P21/00 , C12P21/02 , C12P21/06 , C12R1/19 , C12N15/70
CPC分类号： C07K14/64 , Y10S435/849 , Y10S930/31
摘要： A process is provided for cleaving a polypeptide into at least two polypeptide components comprising treating a reduced, free-cysteine form of the polypeptide with a cleaving agent under conditions for cleaving the polypeptide at a desired junction between the polypeptide cleavage products. More preferably, the process for cleaving comprises culturing cells containing DNA encoding said polypeptide, wherein at least one Asp codon is present in said DNA at a desired junction between the components to be cleaved from each other, said culturing resulting in expression of the DNA to produce the polypeptide in the host cell culture; and treating a reduced, free-cysteine form of the polypeptide with dilute acid under conditions for cleaving the polypeptide at the Asp junction. In particular embodiments, a DNA sequence is provided that encodes a relaxin precursor and includes codons encoding aspartic acid-containing linkers at novel positions within the precursor, allowing the ready cleavage of relaxin A peptides by treatment with dilute acid.
摘要翻译： 提供了一种用于将多肽切割成至少两种多肽组分的方法，包括在多肽切割产物之间的所需连接处切割多肽的条件下用切割剂处理还原的游离半胱氨酸形式的多肽。 更优选地，所述切割方法包括培养含有编码所述多肽的DNA的细胞，其中至少一个Asp密码子存在于所述DNA中在待切割的组分之间的所需连接处，所述培养导致DNA的表达 在宿主细胞培养物中产生多肽; 以及在Asp连接处切割多肽的条件下用稀酸处理还原的游离半胱氨酸形式的多肽。 在具体实施方案中，提供了编码松弛素前体的DNA序列，并且包括在前体内的新位置编码含有天冬氨酸的接头的密码子，从而允许通过用稀酸处理来快速切割松弛素A肽。
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