公开(公告)号：US20120036597A1

公开(公告)日：2012-02-09

申请号：US13197187

申请日：2011-08-03

申请人： Su-May Yu ,  Chin-Fen Su

发明人： Su-May Yu ,  Chin-Fen Su

IPC分类号： A01H5/00 ,  C40B30/04 ,  C12Q1/68 ,  C12N15/82

CPC分类号： C12N15/8273

摘要： Disclosed are chill- or cold-tolerant plants and methods of making the plants. Also disclosed are methods for identifying a plant that is tolerant to chill.

摘要翻译： 公开了冷或冷耐受植物和制造植物的方法。 还公开了用于鉴定耐寒的植物的方法。

公开(公告)号：US07348471B2

公开(公告)日：2008-03-25

申请号：US10819371

申请日：2004-04-05

申请人： Su-May Yu ,  Chwan-Yang Hong

发明人： Su-May Yu ,  Chwan-Yang Hong

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/00

CPC分类号： C12N15/8234 ,  C12N15/8257

摘要： A nucleic acid containing a glutelin gene promoter. Disclosed are transformed plant cells and transgenic plants containing a nucleic acid that includes the promoter operably linked to a sequence encoding heterologous protein. Also disclosed are methods of making the transformed plant cells and transgenic plants and methods for expressing a polypeptide.

摘要翻译： 含有谷蛋白基因启动子的核酸。 公开了转化的植物细胞和含有核酸的转基因植物，所述核酸包括可操作地连接到编码异源蛋白质的序列的启动子。 还公开了制备转化的植物细胞和转基因植物的方法以及用于表达多肽的方法。

公开(公告)号：US07291768B2

公开(公告)日：2007-11-06

申请号：US10630636

申请日：2003-07-30

申请人： Su-May Yu

发明人： Su-May Yu

IPC分类号： C12N15/29 ,  C12N15/82 ,  C12N15/87 ,  A01H5/00

CPC分类号： C12N15/8217 ,  C07K14/415

摘要： A pure polypeptide containing an amino acid sequence at least 70% identical to SEQ ID NO:7, 8, or 9. The polypeptide regulates expression of a gene in a cell. Also disclosed is an isolated nucleic acid characterized in that it hybridizes under stringent conditions to SEQ ID NO:1, 2, or 3, or a complementary sequence thereof. Also within the scope of the invention are a transformed cell or a transgenic plant containing such a nucleic acid and a method of identifying a compound that modulates the activity of the polypeptide.

摘要翻译： 含有与SEQ ID NO：7,8或9至少70％相同的氨基酸序列的纯多肽。该多肽调节基因在细胞中的表达。 还公开了分离的核酸，其特征在于其在严格条件下与SEQ ID NO：1,2或3或其互补序列杂交。 也在本发明的范围内的是含有这种核酸的转化细胞或转基因植物以及鉴定调节多肽活性的化合物的方法。

公开(公告)号：US07094958B2

公开(公告)日：2006-08-22

申请号：US10060515

申请日：2002-01-30

申请人： Su-May Yu

发明人： Su-May Yu

IPC分类号： C12N15/11 ,  C12N15/63 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8234 ,  C12N15/8216 ,  C12N15/8238

摘要： A recombinant nucleic acid containing a transcriptional enhancer from a rice αAmy8 gene, a promoter operably linked to the enhancer, and a coding sequence operably linked to the promoter and encoding a transcript. Also disclosed is a stably transformed plant cell or a transgenic plant containing a recombinant nucleic acid described above, or a similar recombinant nucleic acid with a plurality of a rice αAmy3 enhancer.

公开(公告)号：US06376750B1

公开(公告)日：2002-04-23

申请号：US09575574

申请日：2000-05-22

申请人： Su-May Yu ,  Yu-Chan Chao

发明人： Su-May Yu ,  Yu-Chan Chao

IPC分类号： A01H500

CPC分类号： C12N15/8234 ,  C07K14/415

摘要： The invention relates to the promoter of a rice glycine-rich RNA binding protein gene and its use for expression of heterologous proteins in plant tissues, including tissues of the plant embryo and seedling.

摘要翻译： 本发明涉及水稻富含甘氨酸的RNA结合蛋白基因的启动子及其在植物组织中表达异源蛋白质的用途，包括植物胚胎和幼苗的组织。

公开(公告)号：US06288302B1

公开(公告)日：2001-09-11

申请号：US09072917

申请日：1998-05-04

申请人： Su-May Yu ,  Li-Fei Liu ,  Ming-Tsair Chan

发明人： Su-May Yu ,  Li-Fei Liu ,  Ming-Tsair Chan

IPC分类号： C12N1529

CPC分类号： C12N15/8238 ,  C12N15/8222 ,  C12N15/8234

摘要： Disclosed is a method for producing a transgenic angiosperm plant, particularly a monocot, comprising the step of transforming an immature embryo of an angiosperm plant with a DNA fragment expressible in said angiosperm embryo, said DNA fragment comprising a promoter region derived from an &agr;-amylase gene of a plant, and an exogenous gene encoding a desired gene product, said promoter region being inducible under a sugar-depleted or sugar-free condition to promote the expression of said gene, whereby at least a part of the resultant transgenic angiosperm plant has the ability to express said gene product. Transgenic angiosperm plants and transgenic angiosperm plant seeds are also disclosed. The transgenic angiosperm plant seeds may have application in the industrial production of alcohol, beer, glucose and the like.

摘要翻译： 公开了一种用于生产转基因被子植物，特别是单子叶植物的方法，包括用被子植物胚胎中可表达的DNA片段转化被子植物植物的未成熟胚的步骤，所述DNA片段包含源于α-淀粉酶的启动子区 植物的基因和编码所需基因产物的外源基因，所述启动子区域在无糖或无糖条件下可诱导以促进所述基因的表达，由此所得转基因被子植物的至少一部分具有 表达所述基因产物的能力。 还披露了转基因被子植物和转基因被子植物种子。 转基因被子植物种子可用于工业生产酒精，啤酒，葡萄糖等。

公开(公告)号：US08114443B2

公开(公告)日：2012-02-14

申请号：US12055502

申请日：2008-03-26

申请人： Su-May Yu ,  Ya-Fang Hong

发明人： Su-May Yu ,  Ya-Fang Hong

IPC分类号： A61K38/00 ,  A01H1/00 ,  C07H21/04 ,  C07K14/415 ,  C12N15/00

CPC分类号： C12N15/8223 ,  C12N15/8225 ,  C12N15/8226 ,  C12N15/8257

摘要： Disclosed are a transgenic plant and a transformed plant cell containing a heterologous nucleic acid that includes a sweet potato sporamin SPO promoter operably linked to a sequence encoding a polypeptide. Also disclosed are uses of the plant or cell.

摘要翻译： 公开了一种转基因植物和含有异源核酸的转化植物细胞，其包含与编码多肽的序列可操作地连接的甘薯孢子淀粉SPO启动子。 还公开了植物或细胞的用途。

公开(公告)号：US07411115B2

公开(公告)日：2008-08-12

申请号：US10989719

申请日：2004-11-15

申请人： Su-May Yu

发明人： Su-May Yu

IPC分类号： A01H5/00 ,  C12N15/52 ,  C12N15/82

CPC分类号： C12N15/8257 ,  C12N15/8223 ,  C12N15/8225 ,  C12N15/8226

摘要： A transformed tuberous plant cell or a transgenic tuberous plant containing a heterologous nucleic acid that includes a sweet potato sporamin promoter operably linked to a sequence encoding a polypeptide.

摘要翻译： 转化的块茎植物细胞或含有异源核酸的转基因块茎植物，其包含与编码多肽的序列可操作地连接的甘薯孢子淀粉启动子。

公开(公告)号：US20050066387A1

公开(公告)日：2005-03-24

申请号：US10819371

申请日：2004-04-05

申请人： Su-May Yu ,  Chwan-Yang Hong

发明人： Su-May Yu ,  Chwan-Yang Hong

IPC分类号： C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  C07H21/04 ,  C12N5/04 ,  C12N9/48

CPC分类号： C12N15/8234 ,  C12N15/8257

摘要： A nucleic acid containing a glutelin gene promoter. Disclosed are transformed plant cells and transgenic plants containing a nucleic acid that includes the promoter operably linked to a sequence encoding heterologous protein. Also disclosed are methods of making the transformed plant cells and transgenic plants and methods for expressing a polypeptide.

摘要翻译： 含有谷蛋白基因启动子的核酸。 公开了转化的植物细胞和含有核酸的转基因植物，所述核酸包括可操作地连接到编码异源蛋白质的序列的启动子。 还公开了制备转化的植物细胞和转基因植物的方法以及用于表达多肽的方法。

公开(公告)号：US5969127A

公开(公告)日：1999-10-19

申请号：US951718

申请日：1997-10-16

申请人： Su-May Yu ,  Ming-Tsair Chan

发明人： Su-May Yu ,  Ming-Tsair Chan

IPC分类号： C12N9/32 ,  C12N15/29 ,  C12N15/56 ,  C12N15/84 ,  A01H4/00 ,  C12N15/82

CPC分类号： C12N9/2422

摘要： An expression vector comprising a promoter capable of directing expression of a coding sequence in an angiosperm cell, and a 3' untranslated region of a rice .alpha.-amylase gene, wherein, after the coding sequence is inserted into the vector, the 3' untranslated region and the coding sequence are transcribed into a single mRNA. Also featured are methods of producing a polypeptide in angiosperm cells using such a vector.

摘要翻译： 包含能够引导被子植物细胞中的编码序列表达的启动子和水稻α-淀粉酶基因的3'非翻译区的表达载体，其中在编码序列插入载体后，3'非翻译区 编码序列被转录成单个mRNA。 还特征在于使用这种载体在被子植物细胞中产生多肽的方法。