公开(公告)号：US11788948B2

公开(公告)日：2023-10-17

申请号：US17115657

申请日：2020-12-08

Applicant: ThinkCyte, Inc. ,  University of Tokyo ,  Osaka University

Inventor： Sadao Ota ,  Ryoichi Horisaki ,  Yoko Kawamura ,  Masashi Ugawa ,  Issei Sato

IPC: G01N15/14 ,  G01N15/10

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/145 ,  G01N2015/149 ,  G01N2015/1497

Abstract: The present disclosure provides methods and systems for ghost cytometry (GC), which may be used to produce an image of an object without using a spatially resolving detector. This may be used to perform image-free ultrafast fluorescence “imaging” cytometry, based on, for example, a single pixel detector. Spatial information obtained from the motion of cells relative to a patterned optical structure may be compressively converted into signals that arrive sequentially at a single pixel detector. Combinatorial use of the temporal waveform with the intensity distribution of the random or pseudo-random pattern may permit computational reconstruction of cell morphology. Machine learning methods may be applied directly to the compressed waveforms without image reconstruction to enable efficient image-free morphology-based cytometry. Image-free GC may achieve accurate and high throughput cell classification as well as selective sorting based on cell morphology without a specific biomarker, which have been challenging using conventional flow cytometers.

公开(公告)号：US12259311B2

公开(公告)日：2025-03-25

申请号：US18238368

申请日：2023-08-25

Applicant: ThinkCyte K.K. ,  University of Tokyo ,  Osaka University

Inventor： Sadao Ota ,  Ryoichi Horisaki ,  Yoko Kawamura ,  Masashi Ugawa ,  Issei Sato

IPC: G01N15/14 ,  G01N15/1434 ,  G01N15/10 ,  G01N15/149

Abstract: The present disclosure provides methods and systems for ghost cytometry (GC), which may be used to produce an image of an object without using a spatially resolving detector. This may be used to perform image-free ultrafast fluorescence “imaging” cytometry, based on, for example, a single pixel detector. Spatial information obtained from the motion of cells relative to a patterned optical structure may be compressively converted into signals that arrive sequentially at a single pixel detector. Combinatorial use of the temporal waveform with the intensity distribution of the random or pseudo-random pattern may permit computational reconstruction of cell morphology. Machine learning methods may be applied directly to the compressed waveforms without image reconstruction to enable efficient image-free morphology-based cytometry. Image-free GC may achieve accurate and high throughput cell classification as well as selective sorting based on cell morphology without a specific biomarker, which have been challenging using conventional flow cytometers.

公开(公告)号：US20240133792A1

公开(公告)日：2024-04-25

申请号：US18238368

申请日：2023-08-25

Applicant: ThinkCyte K.K. ,  University of Tokyo ,  Osaka University

Inventor： Sadao Ota ,  Ryoichi Horisaki ,  Yoko Kawamura ,  Masashi Ugawa ,  Issei Sato

IPC: G01N15/14

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N2015/1006

Abstract: The present disclosure provides methods and systems for ghost cytometry (GC), which may be used to produce an image of an object without using a spatially resolving detector. This may be used to perform image-free ultrafast fluorescence “imaging” cytometry, based on, for example, a single pixel detector. Spatial information obtained from the motion of cells relative to a patterned optical structure may be compressively converted into signals that arrive sequentially at a single pixel detector. Combinatorial use of the temporal waveform with the intensity distribution of the random or pseudo-random pattern may permit computational reconstruction of cell morphology. Machine learning methods may be applied directly to the compressed waveforms without image reconstruction to enable efficient image-free morphology-based cytometry. Image-free GC may achieve accurate and high throughput cell classification as well as selective sorting based on cell morphology without a specific biomarker, which have been challenging using conventional flow cytometers.

公开(公告)号：US11412118B2

公开(公告)日：2022-08-09

申请号：US17089028

申请日：2020-11-04

Applicant: Osaka University

Inventor： Ryoichi Horisaki ,  Jun Tanida ,  Riki Egami

IPC: H04N5/225 ,  G01J9/00 ,  G01N21/47

Abstract: An electromagnetic wave phase/amplitude generation device includes a radiation unit configured to radiate electromagnetic waves of a random radiation pattern on a spatial frequency in which a state of the electromagnetic waves to be radiated for each divided region is determined to an imaging object, an imaging unit configured to generate a captured image by imaging scattered electromagnetic waves that are electromagnetic waves generated when the imaging object scatters the electromagnetic waves of the radiation pattern radiated by the radiation unit, and a generation unit configured to generate information indicating at least a phase and amplitude of the electromagnetic waves from the imaging object by performing an arithmetic sparsity constraint operation according to sparsity of the imaging object on the basis of the captured image generated by the imaging unit, information indicating the radiation pattern, and information indicating a signal of the imaging object.