公开(公告)号：US12128409B2

公开(公告)日：2024-10-29

申请号：US17687500

申请日：2022-03-04

申请人： Sony Corporation

发明人： Kazuya Takahashi ,  Tatsumi Ito ,  Yoichi Katsumoto

IPC分类号： B01L3/00 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/149

CPC分类号： B01L3/502761 ,  B01L3/502776 ,  G01N15/1404 ,  G01N15/1484 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/049 ,  G01N2015/1415 ,  G01N15/149

摘要： There is provided a microchip comprising: a main flow path through which a liquid containing microparticles flows; and a branch flow path that branches from the main flow path. A cross-sectional area of a portion of the main flow path is substantially constant up to a branch start position or decreases toward the branch start position, and a radius of curvature R of a side wall that connects a side wall of the main flow path and a side wall of the branch flow path is 0.5 mm or less and more than 0 mm.

公开(公告)号：US20240326050A1

公开(公告)日：2024-10-03

申请号：US18523035

申请日：2023-11-29

申请人： STEVE SHIH ,  KENZA SAMLALI ,  FATEMEH AHMADI

发明人： STEVE SHIH ,  KENZA SAMLALI ,  FATEMEH AHMADI

IPC分类号： B01L3/00 ,  G01N15/10 ,  G01N15/14 ,  G01N15/149

CPC分类号： B01L3/502761 ,  G01N15/1484 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2300/0838 ,  B01L2300/0883 ,  B01L2300/165 ,  B01L2400/0424 ,  G01N2015/1006 ,  G01N15/149

摘要： Microfluidic devices, systems and methods are described herein. The devices, systems and methods provide for trapping particles, including cells. Methods of generating a droplet in a microfluidic device and collecting droplets from microfluidic devices are also disclosed herein.

公开(公告)号：US20240302265A1

公开(公告)日：2024-09-12

申请号：US18570603

申请日：2022-06-16

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Yu-Hwa Lo ,  Xinyu Chen ,  Lauren Waller ,  Jiajie Chen

IPC分类号： G01N15/149 ,  G01N15/10 ,  G01N15/1433 ,  G01N15/1434

CPC分类号： G01N15/149 ,  G01N15/1433 ,  G01N15/1434 ,  G01N2015/1006

摘要： Disclosed are devices, systems and methods for label-free, image-encoded, microfluidics-based cell sorting. In some aspects, an image-based particle sorting system includes an optical imaging system; a data processing system to process the image data obtained by the optical imaging device and determine one or more properties associated with the individual particles flowing in the carrier fluid and to produce a control command based on a comparison of the determined one or more properties with a sorting criteria; and a particle sorting system including a particle flow device that comprises a substrate including the particle-flow channel and a plurality of output channels branching from the particle-flow channel to receive, in one output channel of the plurality of output channels, sorted particles directed by an actuator device based on the control command.

公开(公告)号：US12066369B2

公开(公告)日：2024-08-20

申请号：US18417986

申请日：2024-01-19

申请人： Slingshot Biosciences, Inc.

发明人： Jeffrey Kim ,  Oliver Liu ,  Jeremy Agresti ,  Anh Tuan Nguyen

IPC分类号： G01N15/10 ,  C12Q1/04 ,  G01N1/28 ,  G01N15/14 ,  G01N15/1434 ,  G01N15/01 ,  G01N15/149

CPC分类号： G01N15/1012 ,  C12Q1/04 ,  G01N1/28 ,  G01N15/1434 ,  G01N15/1459 ,  G01N2001/2893 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2015/1014 ,  G01N15/149

摘要： Synthetic human cell mimic hydrogel particles and their use in cytometric or coulter device applications are described. The synthetic human cell mimic hydrogel particles described herein are selectively tunable to have at least one optical, volumetric, or capacitance property that is substantially similar to a corresponding optical, volumetric, or capacitance property of the human cell mimic hydrogel particle's natural biological cell counterpart.

公开(公告)号：US20240272062A1

公开(公告)日：2024-08-15

申请号：US18438518

申请日：2024-02-12

申请人： Owl biomedical, inc.

发明人： Daryl GRUMMITT

IPC分类号： G01N15/149 ,  G01N15/10

CPC分类号： G01N15/149 ,  G01N15/1012 ,  G01N2015/1026

摘要： A MEMS-based particle manipulation system which uses a particle manipulation stage and optical confirmation of the manipulation. The optical confirmation may be camera-based, and may be used to assess the effectiveness or accuracy of the particle manipulation stage. In one exemplary embodiment, the particle manipulation stage is a microfabricated, fluid valve, which sorts a target particle from non-target particles in a fluid stream. The optical confirmation stage is disposed in the microfabricated fluid channels at the input and output of the microfabricated sorting valve. Deep learning techniques are brought to bear on the camera output to increase speed, accuracy and reliability. A calibration device may make use of a pixelated emitter, an optical filter and a spectral separator to mimic the fluorescent output of a biological sample tagged with fluorescent emitters as used in the particle sorting system. This calibration device may allow quick and easy calibration of the optical system for improved speed and performance.

公开(公告)号：US20240198339A1

公开(公告)日：2024-06-20

申请号：US18595927

申请日：2024-03-05

申请人： The General Hospital Corporation

发明人： Ravi Kapur ,  Kyle C. Smith ,  Mehmet Toner

IPC分类号： B01L3/00 ,  A61K35/28 ,  G01N1/40 ,  G01N15/00 ,  G01N15/01 ,  G01N15/02 ,  G01N15/06 ,  G01N15/14 ,  G01N15/149

CPC分类号： B01L3/502761 ,  A61K35/28 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  G01N1/4077 ,  G01N15/0255 ,  G01N15/0618 ,  G01N15/1484 ,  B01L3/502776 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0877 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N2001/4088 ,  G01N2015/0053 ,  G01N15/01 ,  G01N2015/0288 ,  G01N2015/1486 ,  G01N15/149 ,  G01N2015/1493

摘要： A microfluidic device includes: a first microfluidic channel; a second microfluidic channel extending along the first microfluidic channel; and a first array of islands separating the first microfluidic channel from the second microfluidic channel, in which each island is separated from an adjacent island in the array by an opening that fluidly couples the first microfluidic channel to the second microfluidic channel, in which the first microfluidic channel, the second microfluidic channel, and the islands are arranged so that a fluidic resistance of the first microfluidic channel increases relative to a fluidic resistance of the second microfluidic channel along a longitudinal direction of the first microfluidic channel such that, during use of the microfluidic device, a portion of a fluid sample flowing through the first microfluidic channel passes through one or more of the openings between adjacent islands into the second microfluidic channel.

公开(公告)号：US11982611B2

公开(公告)日：2024-05-14

申请号：US16495738

申请日：2018-03-20

申请人： NANOCELLECT BIOMEDICAL, INC.

发明人： Sunghwan Cho ,  Jose Manuel Morachis ,  Ivan Gagne ,  Rick Segil ,  William Arthur Alaynick ,  Zhe Mei ,  Sean Phillips ,  Chien-Chun Yang ,  Dongseob Yun ,  Michael Jerome Benchimol ,  Manna Doud ,  Nicholas Sullivan ,  Constance Ardila

IPC分类号： B01L3/00 ,  B07C5/342 ,  G01N15/14 ,  G01N15/10 ,  G01N15/149

CPC分类号： G01N15/1425 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  B07C5/342 ,  G01N15/1484 ,  B01L2200/0652 ,  B01L2300/0819 ,  B01L2400/082 ,  G01N2015/1006 ,  G01N15/149

摘要： A method includes providing a cartridge and the cartridge includes a slot for receiving a microfluidic chip having a set of first channels. The cartridge also includes a set of second channels and each channel of the set of second channels is coupleable to a different channel of the set of first channels during use with the microfluidic chip. The cartridge also includes an indent configured for engagement and alignment of the cartridge during use. The method also includes inserting the cartridge into a device, such that the cartridge engages a first biasing member of the device configured for alignment of the cartridge in a first direction. The first biasing member is configured to bias movement of the cartridge into locking position with a notch of the device.

公开(公告)号：US20240344960A1

公开(公告)日：2024-10-17

申请号：US18624379

申请日：2024-04-02

申请人： NanoCellect Biomedical, Inc.

发明人： Sunghwan CHO ,  Jose Manuel MORACHIS ,  Ivan GAGNE ,  Rick SEGIL ,  William Arthur ALAYNICK ,  Zhe MEI ,  Sean PHILLIPS ,  Chien-Chun YANG ,  Dongseob YUN ,  Michael Jerome BENCHIMOL ,  Manna DOUD ,  Nicholas SULLIVAN ,  Constance ARDILA

IPC分类号： G01N15/14 ,  B01L3/00 ,  B07C5/342 ,  G01N15/10 ,  G01N15/149

CPC分类号： G01N15/1425 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  B07C5/342 ,  G01N15/1484 ,  B01L2200/0652 ,  B01L2300/0819 ,  B01L2400/082 ,  G01N2015/1006 ,  G01N15/149

摘要： A method includes providing a cartridge and the cartridge includes a slot for receiving a microfluidic chip having a set of first channels. The cartridge also includes a set of second channels and each channel of the set of second channels is coupleable to a different channel of the set of first channels during use with the microfluidic chip. The cartridge also includes an indent configured for engagement and alignment of the cartridge during use. The method also includes inserting the cartridge into a device, such that the cartridge engages a first biasing member of the device configured for alignment of the cartridge in a first direction. The first biasing member is configured to bias movement of the cartridge into locking position with a notch of the device.

公开(公告)号：US20240295484A1

公开(公告)日：2024-09-05

申请号：US18582156

申请日：2024-02-20

申请人： The University of Hong Kong

发明人： Kevin Kin Man TSIA ,  Chak Man LEE

IPC分类号： G01N15/1409 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/149

CPC分类号： G01N15/1409 ,  G01N2015/1006 ,  G01N2015/1413 ,  G01N15/149 ,  G01N2015/1493

摘要： A high-throughput single-file focusing system and methods for polydisperse particles are provided. The system includes a microfluidic device for pre-focusing the polydisperse particles and a high-aspect-ratio (HAR) rectangular structure coupled to the microfluidic device. The microfluidic device includes a fluidic channel configured to localize distributions of the polydisperse particles in a cross-sectional area of the fluidic channel. The dimensions of the fluidic channel are configured to generate a converging secondary flow having four spiral vortices that drives the polydisperse particles to flow inward following a spiral path to be concentrated into a center of each spiral vortex such that the polydisperse particles are focused by the converging secondary flow without any inertial force. The coupled HAR rectangular structure receives the pre-focused polydisperse particles and further confines the particles to form a single file on its mid-plane.

公开(公告)号：USRE50088E1

公开(公告)日：2024-08-20

申请号：US17514186

申请日：2021-11-12

申请人： Sony Corporation

发明人： Masataka Shinoda

IPC分类号： G01N15/1404 ,  B01L3/00 ,  B01L3/02 ,  G01N15/14 ,  G01N15/1434 ,  G01N27/447 ,  G01N15/10 ,  G01N15/1409 ,  G01N15/149

CPC分类号： G01N15/1404 ,  B01L3/0268 ,  B01L3/502761 ,  B01L3/502776 ,  G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N27/44743 ,  G01N27/44791 ,  B01L3/502746 ,  B01L2400/0433 ,  B01L2400/0487 ,  G01N2015/1006 ,  G01N2015/1028 ,  G01N15/1409 ,  G01N2015/1422 ,  G01N2015/1481 ,  G01N2015/1488 ,  G01N15/149 ,  Y10T137/8158

摘要： A microchip is provided that includes a flow path through which a liquid containing a micro particle flows, an orifice through which the liquid flowing through the flow path is discharged into a space outside the microchip, and a light-irradiated portion provided at a predetermined location of the flow path and configured to be irradiated with light. A width of the flow path and a depth of the flow path at the orifice are set to be smaller than a width of the flow path and a depth of the flow path at the light-irradiated portion, and the flow path is configured to gradually decrease from upstream of the orifice in a cross-section area perpendicular to a liquid-delivering direction between the light-irradiated portion and the orifice. A cartridge including the microchip is also provided.