公开(公告)号：US5997913A

公开(公告)日：1999-12-07

申请号：US462080

申请日：1995-06-05

Applicant: Timothy Fowler ,  Christopher C. Barnett ,  Sharon Shoemaker

Inventor： Timothy Fowler ,  Christopher C. Barnett ,  Sharon Shoemaker

IPC: A23L27/24 ,  C12G1/00 ,  C12G1/02 ,  C12H1/15 ,  C12N9/26 ,  C12N9/42 ,  C12P19/00 ,  C12Q1/68 ,  A23B7/10 ,  C12N1/14 ,  C12N9/24

CPC classification number: C12N9/2445 ,  A23L27/25 ,  C12G1/00 ,  C12G1/02 ,  C12N9/2408 ,  C12P19/00 ,  C12Q1/6895 ,  C12R1/885 ,  C12Y302/01021

Abstract: A process for expressing extracellular .beta.-glucosidase in a filamentous fungus by expressing a fungal DNA sequence encoding enhanced, deleted or altered .beta.-glucosidase in a recombinant host microorganism is disclosed. Recombinant fungal cellulase compositions containing enhanced, deleted or altered expression of .beta.-glucosidase is also disclosed.

Abstract translation: 公开了通过在重组宿主微生物中表达编码增强的，缺失的或改变的β-葡糖苷酶的真菌DNA序列来在丝状真菌中表达胞外β-葡糖苷酶的方法。 还公开了含有增强，缺失或改变的β-葡糖苷酶表达的重组真菌纤维素酶组合物。

公开(公告)号：US5874276A

公开(公告)日：1999-02-23

申请号：US448873

申请日：1995-05-24

Applicant: Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund Larenas

Inventor： Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund Larenas

IPC: C12N15/09 ,  A23K1/165 ,  C12N1/15 ,  C12N9/42 ,  C12N15/31 ,  C12N15/80 ,  C12R1/885

CPC classification number: C12Y302/01004 ,  A23K20/189 ,  C12N15/80 ,  C12N9/2437 ,  C12Y302/01021 ,  C12Y302/01091

Abstract: The present invention relates to the cloning and high level expression of novel truncated cellulase proteins or derivatives thereof in the filamentous fungus Trichoderma longibrachiatum. Further aspects of the present invention relate to fungal transformants that express the novel truncated cellulases and derivatives, and expression vectors comprising the DNA gene fragments or variants thereof that code for the truncated cellulases derived from Trichoderma longibrachiatum using genetic engineering techniques.