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1.发明授权Cloning and amplification of the .beta.-glucosidase gene of Trichoderma reesei 失效里氏木霉β-葡萄糖苷酶基因的克隆和扩增
公开(公告)号：US6022725A
公开(公告)日：2000-02-08
申请号：US462090
申请日：1995-06-05
IPC分类号： A23L27/00 , C11D3/386 , C12G1/00 , C12G1/02 , C12N1/15 , C12N9/24 , C12N9/26 , C12N9/42 , C12N15/00 , C12N15/09 , C12N15/55 , C12N15/56 , C12N15/80 , C12P19/00 , C12P19/14 , C12R1/885 , C12Q1/68
CPC分类号： C12R1/885 , C11D3/38645 , C12G1/00 , C12G1/02 , C12N9/2408 , C12N9/2445 , C12P19/00 , C12P19/14 , C12Y302/01021 , Y02E50/16
摘要： A process for expressing extracellular .beta.-glucosidase in a filamentous fungus by expressing a fungal DNA sequence encoding enhanced, deleted or altered .beta.-glucosidase in a recombinant host microorganism is disclosed. Recombinant fungal cellulase compositions containing enhanced, deleted or altered expression of .beta.-glucosidase is also disclosed.
摘要翻译： 公开了通过在重组宿主微生物中表达编码增强的，缺失的或改变的β-葡糖苷酶的真菌DNA序列来在丝状真菌中表达胞外β-葡糖苷酶的方法。 还公开了含有增强，缺失或改变的β-葡糖苷酶表达的重组真菌纤维素酶组合物。
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2.发明授权Method enhancing flavor and aroma in foods by overexpression of .beta.-glucosidase 失效通过β-葡糖苷酶过表达增强食物中的风味和香气的方法
公开(公告)号：US5997913A
公开(公告)日：1999-12-07
申请号：US462080
申请日：1995-06-05
IPC分类号： A23L27/24 , C12G1/00 , C12G1/02 , C12H1/15 , C12N9/26 , C12N9/42 , C12P19/00 , C12Q1/68 , A23B7/10 , C12N1/14 , C12N9/24
CPC分类号： C12N9/2445 , A23L27/25 , C12G1/00 , C12G1/02 , C12N9/2408 , C12P19/00 , C12Q1/6895 , C12R1/885 , C12Y302/01021
摘要： A process for expressing extracellular .beta.-glucosidase in a filamentous fungus by expressing a fungal DNA sequence encoding enhanced, deleted or altered .beta.-glucosidase in a recombinant host microorganism is disclosed. Recombinant fungal cellulase compositions containing enhanced, deleted or altered expression of .beta.-glucosidase is also disclosed.
摘要翻译： 公开了通过在重组宿主微生物中表达编码增强的，缺失的或改变的β-葡糖苷酶的真菌DNA序列来在丝状真菌中表达胞外β-葡糖苷酶的方法。 还公开了含有增强，缺失或改变的β-葡糖苷酶表达的重组真菌纤维素酶组合物。
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3.发明授权Method of detecting DNA encoding a .beta.-glucosidase from a filamentous fungus 失效从丝状真菌中检测编码β-葡糖苷酶的DNA的方法
公开(公告)号：US6103464A
公开(公告)日：2000-08-15
申请号：US463461
申请日：1995-06-05
IPC分类号： A23L27/24 , C12G1/00 , C12G1/02 , C12H1/15 , C12N9/26 , C12N9/42 , C12P19/00 , C12Q1/68 , C07H21/04
CPC分类号： C12N9/2445 , A23L27/25 , C12G1/00 , C12G1/02 , C12N9/2408 , C12P19/00 , C12Q1/6895 , C12R1/885 , C12Y302/01021
摘要： A process for expressing extracellular .beta.-glucosidase in a filamentous fungus by expressing a fungal DNA sequence encoding enhanced, deleted or altered .beta.-glucosidase in a recombinant host microorganism is disclosed. Recombinant fungal cellulase compositions containing enhanced, deleted or altered expression of .beta.-glucosidase is also disclosed.
摘要翻译： 公开了通过在重组宿主微生物中表达编码增强的，缺失的或改变的β-葡糖苷酶的真菌DNA序列来在丝状真菌中表达胞外β-葡糖苷酶的方法。 还公开了含有增强，缺失或改变的β-葡糖苷酶表达的重组真菌纤维素酶组合物。
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4.发明授权公开(公告)号：US06329192B1
公开(公告)日：2001-12-11
申请号：US08070650
申请日：1993-06-01
申请人： Arie Ben-Bassat , Robert Bruner , Sharon Shoemaker , Yehoshua Aloni , Harry Wong , Donald C. Johnson , Amar N. Neogi
发明人： Arie Ben-Bassat , Robert Bruner , Sharon Shoemaker , Yehoshua Aloni , Harry Wong , Donald C. Johnson , Amar N. Neogi
IPC分类号： C12N100
CPC分类号： C12R1/02 , A61L15/28 , C12P19/04 , Y10S435/823 , C08L1/02
摘要： A method and media for producing bacterial cellulose under agitated culture conditions resulting in sustained production over an average of 70 hours of at least 0.1 g/liter per hour are achieved. A unique reticulated cellulose product is produced using the methods and conditions claimed, and may be converted to of a sheet characterized by substantial resistance to densification and great tensile strength when produced by sheet forming means. Acetobacter strains are identified as stable under agitated culture conditions and exhibit substantially reduced gluconic and keto-gluconic acids production.
摘要翻译： 在搅拌培养条件下生产细菌纤维素的方法和介质，得到平均70小时至少0.1g /升/小时的持续生产。 使用所要求保护的方法和条件生产独特的网状纤维素产品，并且可以转化为由片材成型装置生产时具有显着的致密性和大的拉伸强度的片材。 醋酸杆菌菌株在搅拌培养条件下被鉴定为稳定的，并显示出显着降低的葡萄糖酸和酮 - 葡萄糖酸的产生。
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5.发明授权
公开(公告)号：US5821109A
公开(公告)日：1998-10-13
申请号：US442597
申请日：1995-05-17
申请人： Arie Ben-Bassat , Robert Bruner , Sharon Shoemaker , Yehoshua Aloni , Harry Wong , Donald C. Johnson , Amar N. Neogi
发明人： Arie Ben-Bassat , Robert Bruner , Sharon Shoemaker , Yehoshua Aloni , Harry Wong , Donald C. Johnson , Amar N. Neogi
CPC分类号： C12R1/02 , A61L15/28 , C12P19/04 , Y10S435/823
摘要： A method and media for producing bacterial cellulose under agitated culture conditions resulting in sustained production over an average of 70 hours of at least 0.1 g/liter per hour are achieved. A unique reticulated cellulose II product is produced using the methods and conditions claimed, and may be in the form of a sheet characterized by resistance to densification and great tensile strength when produced by sheet forming means. Also strains of Acetobacter that are stable under agitated culture conditions and that exhibit substantially reduced gluconic and keto-gluconic acids production are described.
摘要翻译： 在搅拌培养条件下生产细菌纤维素的方法和介质，得到平均70小时至少0.1g /升/小时的持续生产。 使用所要求保护的方法和条件制备独特的网状纤维素II产品，并且可以是以片材形成装置生产时的抗致密化和抗拉强度特性的片材的形式。 还描述了在搅拌培养条件下稳定并显示出显着降低的葡糖酸和酮 - 葡萄糖酸生产的醋杆菌菌株。
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公开(公告)号：US06429002B1
公开(公告)日：2002-08-06
申请号：US08069458
申请日：1993-06-01
申请人： Arie Ben-Bassat , Robert Bruner , Sharon Shoemaker , Yehoshua Aloni , Harry Wong , Donald C. Johnson , Amar N. Neogi
发明人： Arie Ben-Bassat , Robert Bruner , Sharon Shoemaker , Yehoshua Aloni , Harry Wong , Donald C. Johnson , Amar N. Neogi
IPC分类号： C12N100
CPC分类号： C12R1/02 , A61L15/28 , C12P19/04 , Y10S435/823 , C08L1/02
摘要： Acetobacter strains are identified as stable under agitated culture conditions and exhibit substantially reduced gluconic and keto-gluconic acids production. A method and media for producing bacterial cellulose under agitated culture conditions resulting in sustained production over an average of 70 hours of at least 0.1 g/liter per hour are achieved. A unique reticulated cellulose product is produced using the methods and conditions claimed, and may be converted to of a sheet characterized by substantial resistance to densification and great tensile strength when produced by sheet forming means. Preferred Acetobacter strains are ATCC Nos. 53264, 53263 and 53524.
摘要翻译： 醋酸杆菌菌株在搅拌培养条件下被鉴定为稳定的，并显示出显着降低的葡萄糖酸和酮 - 葡萄糖酸的产生。 在搅拌培养条件下生产细菌纤维素的方法和介质，得到平均70小时至少0.1g /升/小时的持续生产。 使用所要求保护的方法和条件生产独特的网状纤维素产品，并且可以转化为由片材成型装置生产时具有显着的致密性和大的拉伸强度的片材。 优选的醋杆菌属菌株是ATCC Nos.53264,53263和53524。
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7.发明授权Method of producing reticulated cellulose having type II crystalline cellulose 失效生产具有II型结晶纤维素的网状纤维素的方法
公开(公告)号：US5871978A
公开(公告)日：1999-02-16
申请号：US442595
申请日：1995-05-17
申请人： Arie Ben-Bassat , Robert Bruner , Sharon Shoemaker , Yehoshua Aloni , Harry Wong , Donald C. Johnson , Amar N. Neogi
发明人： Arie Ben-Bassat , Robert Bruner , Sharon Shoemaker , Yehoshua Aloni , Harry Wong , Donald C. Johnson , Amar N. Neogi
CPC分类号： C12R1/02 , A61L15/28 , C12P19/04 , Y10S435/823
摘要： A method and media are provided for producing bacterial cellulose under agitated culture conditions resulting in sustained production over an average of 70 hours of at least 0.1 g/liter per hour are achieved. A unique reticulated cellulose product is produced using the methods and conditions claimed, and may be in the form of a sheet characterized by substantial resistance to densification and great tensile strength when produced by sheet forming means. Strains of Acetobacter that are stable under agitated culture conditions and that exhibit substantially reduced gluconic and keto-gluconic acids production are described.
摘要翻译： 提供了一种方法和介质，用于在搅拌的培养条件下生产细菌纤维素，从而实现平均70小时的持续生产，每小时至少0.1g /升。 使用所要求保护的方法和条件制备独特的网状纤维素产品，并且可以是以片材成形方法制造时特征为显着的致密化抗拉强度的片材的形式。 描述了在搅拌培养条件下稳定并且显示出显着降低的葡萄糖酸和酮 - 葡萄糖酸生产的醋杆菌菌株。
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8.发明授权
公开(公告)号：US5144021A
公开(公告)日：1992-09-01
申请号：US633761
申请日：1991-01-30
申请人： Ben-Bassat Arie , Robert Bruner , Sharon Shoemaker , Yehoshua Aloni , Harry Wong , Donald C. Johnson , Amar Neogi
发明人： Ben-Bassat Arie , Robert Bruner , Sharon Shoemaker , Yehoshua Aloni , Harry Wong , Donald C. Johnson , Amar Neogi
CPC分类号： C12R1/02 , A61L15/28 , C12P19/04 , Y10S435/823
摘要： A method and media for producing bacterial cellulose under agitated culture conditions resulting in sustained production over an average of 70 hours of at least 0.1 g/liter per hour are achieved. A unique reticulated cellulose product is produced using the methods and conditions claimed, and may be in the form of a sheet characterized by substantial resistance to densification and great tensile strength when produced by sheet forming means.Strains of Acetobacter that are stable under agitated culture conditions and that exhibit substantially reduced gluconic and keto-gluconic acid production are described.
摘要翻译： 在搅拌培养条件下生产细菌纤维素的方法和介质，得到平均70小时至少0.1g /升/小时的持续生产。 使用所要求保护的方法和条件制备独特的网状纤维素产品，并且可以是以片材成形方法制造时特征为显着的致密化抗拉强度的片材的形式。 描述了在搅拌培养条件下稳定并且显示出显着降低的葡萄糖酸和酮 - 葡萄糖酸生产的醋杆菌菌株。
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9.发明授权Detergent compositions containing cellulase compositions deficient in CBH I type components 失效含有缺乏CBH I型组分的纤维素酶组合物的洗涤剂组合物
公开(公告)号：US6162782A
公开(公告)日：2000-12-19
申请号：US463518
申请日：1995-06-05
CPC分类号： C11D3/001 , C11D3/38645 , C12N9/2437 , C12N15/80 , C12Y302/01004 , D06M16/003 , D06M2200/50
摘要： Disclosed are cellulase compositions containing one or more endoglucanase components and less than about 5 weight percent of CBH I type components. When incorporated into detergent compositions and used in acidic, neutral, or alkaline washing media, such cellulase compositions impart color retention/restoration properties as well as improved softening properties to cotton-containing fabrics. Additionally, such compositions impart reduced strength loss to cotton-containing fabrics as compared to cellulase compositions containing greater amounts of CBH I type components.
摘要翻译： 公开了含有一种或多种内切葡聚糖酶组分和小于约5重量％的CBH I型组分的纤维素酶组合物。 当结合到洗涤剂组合物中并用于酸性，中性或碱性洗涤介质中时，这种纤维素酶组合物赋予含棉织物的保色性/恢复性以及改善的软化性能。 另外，与含有较大量的CBH I型组分的纤维素酶组合物相比，这种组合物赋予含棉织物强度损失降低。
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10.发明授权
公开(公告)号：US6107265A
公开(公告)日：2000-08-22
申请号：US152099
申请日：1993-11-15
CPC分类号： C11D3/001 , C11D3/38645 , C12N15/80 , C12N9/2437 , C12Y302/01004 , D06M16/003 , D06M2200/50
摘要： Disclosed are cellulase compositions containing one or more endoglucanase components and less than about 5 weight percent of CBH I type components. When incorporated into detergent compositions and used in acidic, neutral, or alkaline washing media, such cellulase compositions impart color retention/restoration properties as well as improved softening properties to cotton-containing fabrics. Additionally, such compositions impart reduced strength loss to cotton-containing fabrics as compared to cellulase compositions containing greater amounts of CBH I type components.
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