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公开(公告)号：US20170306372A1
公开(公告)日：2017-10-26
申请号：US15513615
申请日：2015-09-18
CPC分类号： C12P19/14 , C12P19/02 , C12Y302/01004 , C12Y302/01008
摘要： A method prepares a sugar solution from cellulose-containing biomass. The method includes step (1): a step of obtaining an endoxylanase hydrolysate by hydrolyzing the cellulose-containing biomass using endoxylanase derived from microorganisms of the Acremonium genus or the Aspergillus genus, step (2): a step of separating the endoxylanase hydrolysate into an endoxylanase hydrolysate solid and an endoxylanase hydrolysate liquid through solid-liquid separation, step (3): a step of obtaining a cellulase hydrolysate by hydrolyzing the endoxylanase hydrolysate solid using cellulase derived from a filamentous fungus, and step (4): a step of filtering the cellulase hydrolysate through an ultrafiltration membrane to recover a sugar solution from the filtrate side and collect an enzyme component from the non-filtrate side.
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公开(公告)号：US20160010130A1
公开(公告)日：2016-01-14
申请号：US14767036
申请日：2014-02-19
摘要： A method of producing a sugar solution includes (1) to (3): (1) preparing a slurry of a cellulose-containing biomass pretreated material and an inactivated cellulose, (2) hydrolyzing the slurry in (1) by adding a filamentous fungus-derived cellulase to the slurry, and (3) separating a hydrolysate in (2) into a solution component and a hydrolysis residue through solid-liquid separation, and filtering the solution component through an ultrafiltration membrane, thereby recovering the filamentous fungus-derived cellulase as a non-filtrate and the sugar solution as a filtrate.
摘要翻译： 制备糖溶液的方法包括（1）〜（3）：（1）制备含纤维素的生物质预处理物质和灭活纤维素的浆料，（2）通过添加丝状真菌 （3）通过固液分离将（2）中的水解产物分离成溶液组分和水解残余物，并通过超滤膜过滤溶液组分，从而回收丝状真菌衍生的纤维素酶 作为非滤液和糖溶液作为滤液。
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公开(公告)号：US09193963B2
公开(公告)日：2015-11-24
申请号：US14195923
申请日：2014-03-04
发明人： Hiroyuki Kurihara , Takeshi Tsukada , Katsushige Yamada , Yumiko Mishima , Yuka Maeno , Kazuhiko Ishikawa
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C13K1/02
摘要： Endoglucanase characterized by a decreased degree of activity inhibition by a lignin-derived aromatic compound, and prepared by substituting tryptophan at position 273 in the amino acid sequence of wild-type thermophilic bacterium-derived endoglucanase with an amino acid other than aromatic amino acids.
摘要翻译： 内切葡聚糖酶的特征在于木质素衍生的芳族化合物的活性抑制程度降低，并且通过用野生型嗜热细菌来源的内切葡聚糖酶的氨基酸序列中的位置273处的色氨酸与除芳香族氨基酸以外的氨基酸取代。
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24.发明申请公开(公告)号：US20130273608A1
公开(公告)日：2013-10-17
申请号：US13916190
申请日：2013-06-12
CPC分类号： C12P19/40 , C12P7/04 , C12P7/06 , C12P7/16 , C12P7/56 , C12P13/001 , C12P19/04 , C12P19/14 , Y02E50/16 , Y02E50/17
摘要： A method of producing a compound originating from a polysaccharide-based biomass includes at least one of a saccharification step that produces a sugar solution containing a monosaccharide and/or an oligosaccharide from a product obtainable by hydrolyzing the polysaccharide-based biomass; a fermentation step that ferments the sugar solution containing the monosaccharide and/or oligosaccharide originating from the polysaccharide-based biomass; and a treatment that removes a fermentation inhibitor with the use of a separation membrane having a glucose removal rate and an isopropyl alcohol removal rate which simultaneously satisfy the following relationships (I) and (II) when a 500 ppm aqueous glucose solution at pH 6.5 at 25° C. and a 500 ppm aqueous isopropyl alcohol solution at pH 6.5 at 25° C. are respectively permeated through the membrane at an operation pressure of 0.5 MPa, prior to the saccharification step and/or in the step prior to the fermentation step: Glucose removal rate≧80% (I) Glucose removal rate−Isopropyl alcohol removal rate≧20% (II).
摘要翻译： 一种生产源自多糖类生物质的化合物的方法包括从通过水解多糖类生物质获得的产物产生含有单糖和/或寡糖的糖溶液的糖化步骤中的至少一种; 发酵步骤，其发酵含有源自多糖类生物质的单糖和/或寡糖的糖溶液; 以及当pH为6.5的500ppm葡萄糖水溶液时，使用具有葡萄糖去除速率和异丙醇除去速率的分离膜除去发酵抑制剂的处理，其同时满足以下关系式（I）和（II） 25℃下的pH为6.5的500ppm异丙醇水溶液分别在糖化步骤之前和/或在发酵步骤之前的步骤中以0.5MPa的操作压力渗透通过膜 ：葡萄糖去除率> = 80％（I）葡萄糖去除率 - 异丙醇去除率> = 20％（II）。
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公开(公告)号：US10781466B2
公开(公告)日：2020-09-22
申请号：US15508558
申请日：2015-09-04
发明人： Atsushi Minamino , Yuka Asahi , Hiroyuki Kurihara , Jumpei Kishimoto , Masashi Higasa , Katsushige Yamada
摘要： A method of producing a sugar liquid includes: (A) reacting mannanase with a liquid component obtained by hydrolysis treatment of woody biomass to obtain a saccharified liquid; and (B) filtering the saccharified liquid in Step (A) through a microfiltration membrane and/or ultrafiltration membrane to collect a sugar liquid from a permeate side.
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公开(公告)号：US10266860B2
公开(公告)日：2019-04-23
申请号：US15454242
申请日：2017-03-09
摘要： An apparatus that produces a sugar liquid by a method including hydrolyzing cellulose includes a hydrolysis tank to which a recovered enzyme feed pipe and a fresh enzyme feed pipe are connected; a device for solid-liquid separation of a hydrolysate; a sugar liquid-retaining tank having a water supply pipe that washes an ultrafiltration membrane and/or removes recovered enzyme retained in a circulation pipe; and an ultrafiltration membrane device that separates enzyme and a sugar liquid.
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公开(公告)号：US09920346B2
公开(公告)日：2018-03-20
申请号：US15513615
申请日：2015-09-18
CPC分类号： C12P19/14 , C12P19/02 , C12Y302/01004 , C12Y302/01008
摘要： A method prepares a sugar solution from cellulose-containing biomass. The method includes step (1): a step of obtaining an endoxylanase hydrolysate by hydrolyzing the cellulose-containing biomass using endoxylanase derived from microorganisms of the Acremonium genus or the Aspergillus genus, step (2): a step of separating the endoxylanase hydrolysate into an endoxylanase hydrolysate solid and an endoxylanase hydrolysate liquid through solid-liquid separation, step (3): a step of obtaining a cellulase hydrolysate by hydrolyzing the endoxylanase hydrolysate solid using cellulase derived from a filamentous fungus, and step (4): a step of filtering the cellulase hydrolysate through an ultrafiltration membrane to recover a sugar solution from the filtrate side and collect an enzyme component from the non-filtrate side.
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公开(公告)号：US20170275663A1
公开(公告)日：2017-09-28
申请号：US15508558
申请日：2015-09-04
发明人： Atsushi Minamino , Yuka Asahi , Hiroyuki Kurihara , Jumpei Kishimoto , Masashi Higasa , Katsushige Yamada
摘要： A method of producing a sugar liquid includes: (A) reacting mannanase with a liquid component obtained by hydrolysis treatment of woody biomass to obtain a saccharified liquid; and (B) filtering the saccharified liquid in Step (A) through a microfiltration membrane and/or ultrafiltration membrane to collect a sugar liquid from a permeate side.
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公开(公告)号：US09605291B2
公开(公告)日：2017-03-28
申请号：US14767036
申请日：2014-02-19
摘要： A method of producing a sugar solution includes (1) to (3): (1) preparing a slurry of a cellulose-containing biomass pretreated material and an inactivated cellulose, (2) hydrolyzing the slurry in (1) by adding a filamentous fungus-derived cellulase to the slurry, and (3) separating a hydrolysate in (2) into a solution component and a hydrolysis residue through solid-liquid separation, and filtering the solution component through an ultrafiltration membrane, thereby recovering the filamentous fungus-derived cellulase as a non-filtrate and the sugar solution as a filtrate.
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公开(公告)号：US09527802B2
公开(公告)日：2016-12-27
申请号：US14419550
申请日：2013-08-08
IPC分类号： C07C231/24 , C07C235/34
CPC分类号： C07C231/24 , C07C235/34
摘要： A method of manufacturing coumaramide includes treating cellulose-based biomass with an ammonia-containing treatment agent to obtain ammonia-treated biomass, immersing the ammonia-treated biomass into a solvent containing at least water and eluting coumaramide in the ammonia-treated biomass into the solvent to obtain a coumaramide solution, and precipitating coumaramide in the coumaramide solution as a crystal to obtain a coumaramide crystal.
摘要翻译： 制造香豆素的方法包括用含氨处理剂处理纤维素类生物质，得到经氨处理的生物质，将经氨处理的生物质浸入含有至少水的溶剂中，并将氨处理的生物质中的香豆酰胺洗脱到溶剂中 得到香豆素溶液，并将作为结晶的香豆酰胺溶液中的香豆酰胺沉淀，得到香豆素结晶。
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