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公开(公告)号：US09193963B2
公开(公告)日：2015-11-24
申请号：US14195923
申请日：2014-03-04
发明人： Hiroyuki Kurihara , Takeshi Tsukada , Katsushige Yamada , Yumiko Mishima , Yuka Maeno , Kazuhiko Ishikawa
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C13K1/02
摘要： Endoglucanase characterized by a decreased degree of activity inhibition by a lignin-derived aromatic compound, and prepared by substituting tryptophan at position 273 in the amino acid sequence of wild-type thermophilic bacterium-derived endoglucanase with an amino acid other than aromatic amino acids.
摘要翻译： 内切葡聚糖酶的特征在于木质素衍生的芳族化合物的活性抑制程度降低，并且通过用野生型嗜热细菌来源的内切葡聚糖酶的氨基酸序列中的位置273处的色氨酸与除芳香族氨基酸以外的氨基酸取代。
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公开(公告)号：US10435728B2
公开(公告)日：2019-10-08
申请号：US15521654
申请日：2015-11-05
发明人： Hiroyuki Kurihara , Takuya Kasahara , Chiaki Yamada , Natsuko Murakami , Katsushige Yamada , Kazuhiko Ishikawa , Masahiro Watanabe
摘要： An endoxylanase mutant has improved thermal stability. The endoxylanase mutant having endoxylanase activity includes an amino acid sequence at least including substitution of an amino acid residue at one or more positions selected from positions corresponding to position 35, position 44, position 62, position 63, position 101, and position 102 of an amino acid sequence of SEQ ID NO: 1 in an amino acid sequence of endoxylanase derived from a filamentous fungus.
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3.发明申请公开(公告)号：US20170240941A1
公开(公告)日：2017-08-24
申请号：US15521654
申请日：2015-11-05
发明人： Hiroyuki Kurihara , Takuya Kasahara , Chiaki Yamada , Natsuko Murakami , Katsushige Yamada , Kazuhiko Ishikawa , Masahiro Watanabe
CPC分类号： C12P19/14 , C12N5/00 , C12N9/2482 , C12N15/09 , C12P19/02 , C12Y302/01008
摘要： An endoxylanase mutant has improved thermal stability. The endoxylanase mutant having endoxylanase activity includes an amino acid sequence at least including substitution of an amino acid residue at one or more positions selected from positions corresponding to position 35, position 44, position 62, position 63, position 101, and position 102 of an amino acid sequence of SEQ ID NO: 1 in an amino acid sequence of endoxylanase derived from a filamentous fungus.
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公开(公告)号：US20200002695A1
公开(公告)日：2020-01-02
申请号：US16485205
申请日：2018-02-23
发明人： Natsuko Murakami , Koji Kobayashi , Hiroyuki Kurihara , Katsushige Yamada , Masahiro Watanabe , Keiko Uechi , Tamotsu Hoshino
IPC分类号： C12N9/24
摘要： A xylanase variant includes an amino acid sequence derived from an amino acid sequence of a xylanase from a filamentous fungus wherein one or more amino acid residues at the positions selected from the positions corresponding to position 78, position 80, position 117, position 155, position 169, and position 203 in the amino acid sequence of SEQ ID NO: 1 are substituted, the xylanase variant having a xylanase activity.
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公开(公告)号：US20140178947A1
公开(公告)日：2014-06-26
申请号：US14195923
申请日：2014-03-04
发明人： Hiroyuki Kurihara , Takeshi Tsukada , Katsushige Yamada , Yumiko Mishima , Yuka Maeno , Kazuhiko Ishikawa
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C13K1/02
摘要： Endoglucanase characterized by a decreased degree of activity inhibition by a lignin-derived aromatic compound, and prepared by substituting tryptophan at position 273 in the amino acid sequence of wild-type thermophilic bacterium-derived endoglucanase with an amino acid other than aromatic amino acids.
摘要翻译： 内切葡聚糖酶的特征在于木质素衍生的芳族化合物的活性抑制程度降低，并且通过用野生型嗜热细菌来源的内切葡聚糖酶的氨基酸序列中的位置273处的色氨酸与除芳香族氨基酸以外的氨基酸取代。
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公开(公告)号：US20200216869A1
公开(公告)日：2020-07-09
申请号：US16089057
申请日：2017-03-30
摘要： A method produces xylo-oligosaccharides from a biomass containing xylan and cellulose, which method is convenient and has a high yield of xylo-oligosaccharides because of inhibition of degradation of xylo-oligosaccharides into xylose. In the method of producing xylo-oligosaccharides, a biomass containing xylan and cellulose is hydrolyzed with a cellulase composition having at least activities of xylanase, cellobiohydrolase and β-glucosidase and substantially free of β-xylosidase activity during hydrolysis.
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公开(公告)号：US10563238B2
公开(公告)日：2020-02-18
申请号：US14913404
申请日：2014-08-21
摘要： A method of producing a sugar liquid includes steps (1) providing a pretreatment product of a first cellulose-containing biomass; (2) adding a second cellulose-containing biomass or a pretreatment product thereof in an amount ranging from 1 to 50% based on the solid content weight of the first cellulose-containing biomass pretreatment product of step (1), and carrying out hydrolysis by a filamentous fungus-derived cellulase; and (3) subjecting a hydrolysate of step (2) to solid-liquid separation to obtain a solution component and a hydrolysis residue, and filtering the solution component through an ultrafiltration membrane to recover the filamentous fungus-derived cellulase as a retentate and recover a sugar liquid as a permeate.
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公开(公告)号：US10519093B2
公开(公告)日：2019-12-31
申请号：US16090132
申请日：2017-03-28
发明人： Atsushi Minamino , Shigeyuki Funada , Hiroyuki Kurihara , Yuka Asahi , Takuya Kasahara , Katsushige Yamada
IPC分类号： C07C45/00 , C07C51/00 , C07C59/00 , C07C51/47 , C07C45/79 , C07C47/58 , C07C59/52 , C07C59/64 , C07C51/43 , C07C65/03
摘要： A method efficiently produces hydroxycinnamic acids having a high quality, from a cellulose-containing biomass. More specifically, the method includes the steps of: obtaining an alkaline filtrate by allowing an alkaline aqueous medium to pass through a cellulose-containing biomass; obtaining a hydroxycinnamic acid extraction liquid by allowing the alkaline filtrate to repeatedly pass through the cellulose-containing biomass; and separating the hydroxycinnamic acid from the hydroxycinnamic acid extraction liquid.
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公开(公告)号：US10385302B2
公开(公告)日：2019-08-20
申请号：US14759513
申请日：2014-01-06
摘要： A device that produces a sugar solution from cellulose-containing biomass includes: a horizontal reaction tank that includes a stirring shaft provided along a horizontal direction in the horizontal reaction tank and a stirring blade provided to the stirring shaft, the horizontal reaction tank being configured to obtain a saccharified slurry by reacting the cellulose-containing biomass with a saccharification enzyme by stirring the cellulose-containing biomass and the saccharification enzyme; a vertical reaction tank configured to obtain a saccharified liquid by saccharifying the saccharified slurry; a saccharified slurry feed line that connects the horizontal reaction tank and the vertical reaction tank; and a warming part provided around the horizontal reaction tank or on a wall surface of the horizontal reaction tank and heats the horizontal reaction tank.
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公开(公告)号：US20190112254A1
公开(公告)日：2019-04-18
申请号：US16090132
申请日：2017-03-28
发明人： Atsushi Minamino , Shigeyuki Funada , Hiroyuki Kurihara , Yuka Asahi , Takuya Kasahara , Katsushige Yamada
摘要： A method efficiently produces hydroxycinnamic acids having a high quality, from a cellulose-containing biomass. More specifically, the method includes the steps of: obtaining an alkaline filtrate by allowing an alkaline aqueous medium to pass through a cellulose-containing biomass; obtaining a hydroxycinnamic acid extraction liquid by allowing the alkaline filtrate to repeatedly pass through the cellulose-containing biomass; and separating the hydroxycinnamic acid from the hydroxycinnamic acid extraction liquid.
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