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公开(公告)号:US09605291B2
公开(公告)日:2017-03-28
申请号:US14767036
申请日:2014-02-19
摘要: A method of producing a sugar solution includes (1) to (3): (1) preparing a slurry of a cellulose-containing biomass pretreated material and an inactivated cellulose, (2) hydrolyzing the slurry in (1) by adding a filamentous fungus-derived cellulase to the slurry, and (3) separating a hydrolysate in (2) into a solution component and a hydrolysis residue through solid-liquid separation, and filtering the solution component through an ultrafiltration membrane, thereby recovering the filamentous fungus-derived cellulase as a non-filtrate and the sugar solution as a filtrate.
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公开(公告)号:US20150376669A1
公开(公告)日:2015-12-31
申请号:US14765416
申请日:2014-03-03
CPC分类号: C12P19/14 , B01D61/145 , B01D61/147 , B01D2311/25 , B01D2311/2688 , C12P19/02 , C12P19/04 , C12P2201/00 , C12P2203/00 , C13K1/02
摘要: A method of producing sugar solution by repeating a sugar solution production process includes steps (1) to (3), wherein a wash solution obtained in step (4) of washing a separation membrane after step (3) is used for step (1) of subsequent sugar solution production processes: step (1): preparing slurry of pretreated cellulose-containing biomass; step (2): hydrolyzing the slurry of pretreated cellulose-containing biomass in step (1) using cellulase from filamentous fungi; and step (3): subjecting the hydrolyzate of step (2) to solid-liquid separation into a solution component and a hydrolysis residue, filtering the solution component through an ultrafiltration membrane and recovering the cellulase from filamentous fungi as a non-permeate, and recovering the sugar solution as a permeate.
摘要翻译: 通过重复糖溶液制备方法制备糖溶液的方法包括步骤(1)至(3),其中步骤(3)中使用步骤(4)中洗涤分离膜获得的洗涤溶液用于步骤(1) 的后续糖溶液生产方法:步骤(1):制备预处理含纤维素生物质的浆料; 步骤(2):使用来自丝状真菌的纤维素酶在步骤(1)中水解预处理的含纤维素生物质的浆料; 和步骤(3):将步骤(2)的水解产物进行固液分离成溶液成分和水解残余物,通过超滤膜过滤溶液成分,并从丝状真菌中回收纤维素酶作为非渗透物,以及 回收作为渗透物的糖溶液。
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公开(公告)号:US10253343B2
公开(公告)日:2019-04-09
申请号:US15521990
申请日:2015-10-29
摘要: A method of producing a sugar liquid and a xylooligosaccharide includes Steps (1) to (3): Step (1): hydrolyzing a cellulose-containing biomass with a filamentous fungus-derived cellulase; Step (2): subjecting the hydrolysate of Step (1) to solid-liquid separation, and filtering the solution component through an ultrafiltration membrane to recover cellulase as a non-permeate, and to recover a sugar liquid as a permeate; and Step (3): reacting the recovered cellulase in Step (2) with a xylan-containing material, and recovering a xylooligosaccharide produced, Step (3) being independent from Step (1).
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公开(公告)号:US20170314051A1
公开(公告)日:2017-11-02
申请号:US15521990
申请日:2015-10-29
CPC分类号: C12P19/14 , C12P19/02 , C12P19/12 , C12Y302/01004 , C12Y302/01008
摘要: A method of producing a sugar liquid and a xylooligosaccharide includes Steps (1) to (3): Step (1): hydrolyzing a cellulose-containing biomass with a filamentous fungus-derived cellulase; Step (2): subjecting the hydrolysate of Step (1) to solid-liquid separation, and filtering the solution component through an ultrafiltration membrane to recover cellulase as a non-permeate, and to recover a sugar liquid as a permeate; and Step (3): reacting the recovered cellulase in Step (2) with a xylan-containing material, and recovering a xylooligosaccharide produced, Step (3) being independent from Step (1).
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公开(公告)号:US20160010130A1
公开(公告)日:2016-01-14
申请号:US14767036
申请日:2014-02-19
摘要: A method of producing a sugar solution includes (1) to (3): (1) preparing a slurry of a cellulose-containing biomass pretreated material and an inactivated cellulose, (2) hydrolyzing the slurry in (1) by adding a filamentous fungus-derived cellulase to the slurry, and (3) separating a hydrolysate in (2) into a solution component and a hydrolysis residue through solid-liquid separation, and filtering the solution component through an ultrafiltration membrane, thereby recovering the filamentous fungus-derived cellulase as a non-filtrate and the sugar solution as a filtrate.
摘要翻译: 制备糖溶液的方法包括(1)〜(3):(1)制备含纤维素的生物质预处理物质和灭活纤维素的浆料,(2)通过添加丝状真菌 (3)通过固液分离将(2)中的水解产物分离成溶液组分和水解残余物,并通过超滤膜过滤溶液组分,从而回收丝状真菌衍生的纤维素酶 作为非滤液和糖溶液作为滤液。
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公开(公告)号:US10435728B2
公开(公告)日:2019-10-08
申请号:US15521654
申请日:2015-11-05
发明人: Hiroyuki Kurihara , Takuya Kasahara , Chiaki Yamada , Natsuko Murakami , Katsushige Yamada , Kazuhiko Ishikawa , Masahiro Watanabe
摘要: An endoxylanase mutant has improved thermal stability. The endoxylanase mutant having endoxylanase activity includes an amino acid sequence at least including substitution of an amino acid residue at one or more positions selected from positions corresponding to position 35, position 44, position 62, position 63, position 101, and position 102 of an amino acid sequence of SEQ ID NO: 1 in an amino acid sequence of endoxylanase derived from a filamentous fungus.
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公开(公告)号:US20170240941A1
公开(公告)日:2017-08-24
申请号:US15521654
申请日:2015-11-05
发明人: Hiroyuki Kurihara , Takuya Kasahara , Chiaki Yamada , Natsuko Murakami , Katsushige Yamada , Kazuhiko Ishikawa , Masahiro Watanabe
CPC分类号: C12P19/14 , C12N5/00 , C12N9/2482 , C12N15/09 , C12P19/02 , C12Y302/01008
摘要: An endoxylanase mutant has improved thermal stability. The endoxylanase mutant having endoxylanase activity includes an amino acid sequence at least including substitution of an amino acid residue at one or more positions selected from positions corresponding to position 35, position 44, position 62, position 63, position 101, and position 102 of an amino acid sequence of SEQ ID NO: 1 in an amino acid sequence of endoxylanase derived from a filamentous fungus.
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公开(公告)号:US20150167037A1
公开(公告)日:2015-06-18
申请号:US14412217
申请日:2013-07-01
CPC分类号: C12P19/14 , C12N9/2434 , C12N9/2437 , C12P19/02 , C13K1/02 , C13K1/04
摘要: A method of producing a sugar liquid from a cellulose-containing biomass includes (1) hydrolyzing a cellulose-containing biomass using a filamentous fungus-derived cellulose and carrying out solid-liquid separation into a sugar liquid and a hydrolysis residue; (2) washing the hydrolysis residue in (1) using an aqueous alkaline solution and an aqueous inorganic salt solution independently and recovering, as a washing liquid, the filamentous fungus-derived cellulase adsorbed to the hydrolysis residue; and (3) filtering the washing liquid in (2) through an ultrafiltration membrane to recover a sugar liquid as a permeate and the filamentous fungus-derived cellulase as a non-permeate.
摘要翻译: 从含纤维素生物质生产糖液的方法包括:(1)使用丝状真菌衍生的纤维素水解含纤维素的生物质并进行固液分离成糖液和水解残余物; (2)使用碱性水溶液和无机盐水溶液独立地洗涤(1)中的水解残余物,作为洗涤液回收吸附在水解残渣上的丝状真菌衍生的纤维素酶; (3)通过超滤膜过滤(2)中的洗涤液,回收作为渗透物的糖液和作为非渗透物的丝状真菌来源的纤维素酶。
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公开(公告)号:US20200216869A1
公开(公告)日:2020-07-09
申请号:US16089057
申请日:2017-03-30
摘要: A method produces xylo-oligosaccharides from a biomass containing xylan and cellulose, which method is convenient and has a high yield of xylo-oligosaccharides because of inhibition of degradation of xylo-oligosaccharides into xylose. In the method of producing xylo-oligosaccharides, a biomass containing xylan and cellulose is hydrolyzed with a cellulase composition having at least activities of xylanase, cellobiohydrolase and β-glucosidase and substantially free of β-xylosidase activity during hydrolysis.
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公开(公告)号:US10563238B2
公开(公告)日:2020-02-18
申请号:US14913404
申请日:2014-08-21
摘要: A method of producing a sugar liquid includes steps (1) providing a pretreatment product of a first cellulose-containing biomass; (2) adding a second cellulose-containing biomass or a pretreatment product thereof in an amount ranging from 1 to 50% based on the solid content weight of the first cellulose-containing biomass pretreatment product of step (1), and carrying out hydrolysis by a filamentous fungus-derived cellulase; and (3) subjecting a hydrolysate of step (2) to solid-liquid separation to obtain a solution component and a hydrolysis residue, and filtering the solution component through an ultrafiltration membrane to recover the filamentous fungus-derived cellulase as a retentate and recover a sugar liquid as a permeate.
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