公开(公告)号：US20050009156A1

公开(公告)日：2005-01-13

申请号：US10914242

申请日：2004-08-10

申请人： Tetsuya Yano ,  Takeshi Imamura ,  Sakae Suda ,  Tsutomu Honma

发明人： Tetsuya Yano ,  Takeshi Imamura ,  Sakae Suda ,  Tsutomu Honma

IPC分类号： C12N1/21 ,  C12N9/10 ,  C12P7/62 ,  C07H21/04 ,  C12N9/18

CPC分类号： C12N9/1025 ,  C12P7/625

摘要： The present invention provides a PHA (polyhydroxyalkanoate) synthase useful in a process for preparing a PHA, a gene encoding the enzyme, a recombinant vector comprising the gene, a transformant transformed by the vector, a process for producing a PHA synthase utilizing the transformant and a process for preparing a PHA utilizing the transformant. A transformant obtained by introducing a PHA synthase gene from Pseudomonas putida P91 strain into a host microorganism is cultured to produce a PHA synthase or PHA.

公开(公告)号：US07056708B2

公开(公告)日：2006-06-06

申请号：US10410349

申请日：2003-04-10

申请人： Takashi Kenmoku ,  Etsuko Sugawa ,  Tetsuya Yano ,  Takeshi Imamura ,  Tsutomu Honma

发明人： Takashi Kenmoku ,  Etsuko Sugawa ,  Tetsuya Yano ,  Takeshi Imamura ,  Tsutomu Honma

IPC分类号： C12P11/00 ,  C12P7/62

CPC分类号： C08G63/6882 ,  C12P7/625 ,  C12P11/00

摘要： The present invention provides a method of effectively producing polyhydroxyalkanoate having an aromatic substituted residue in its monomer unit. In the method of producing polyhydroxyalkanoate by using microorganisms, microorganisms capable of producing polyhydroxyalkanoate are cultured in a culture medium containing at least one starting compound selected from the group consisting of substituted alkanes represented by formula (1) to produce polyhydroxyalkanoate having, in its molecule, at least one unit selected from the group consisting of 3-hydroxy-substituted alkanoate units represented by formula (2): R—(CH2)n—CH2—CH2—CH3  (1) wherein R represents a residue containing a substituted aromatic ring, and n represents any integer of 1 to 8; wherein R represents a residue containing a substituted aromatic ring, and n represents any integer of 1 to 8. In formulas (1) and (2), a residue R is as described in the specification.

摘要翻译： 本发明提供了在其单体单元中有效制备具有芳族取代残基的聚羟基链烷酸酯的方法。 在通过使用微生物制备聚羟基链烷酸酯的方法中，能够在含有选自由式（1）表示的取代烷烃中的至少一种起始化合物的培养基中培养能够产生聚羟基链烷酸酯的微生物，从而产生在其分子中具有的聚羟基链烷酸酯， 选自由式（2）表示的3-羟基取代的链烷酸酯单元中的至少一个单元：<？in-line-formula description =“In-line Formulas”end =“lead”？> R-（CH （2）-CH 2 CH 2 CH 2 CH 2（1） 其中，R表示含有取代芳环的残基，n表示1〜8的整数，n表示1〜8的整数。 其中R表示含有取代芳环的残基，n表示1至8的任何整数。在式（1）和（2）中，残基R如说明书所述。

公开(公告)号：US06777153B2

公开(公告)日：2004-08-17

申请号：US10105305

申请日：2002-03-26

申请人： Tetsuya Yano ,  Etsuko Sugawa ,  Takeshi Imamura ,  Tsutomu Honma ,  Takashi Kenmoku

发明人： Tetsuya Yano ,  Etsuko Sugawa ,  Takeshi Imamura ,  Tsutomu Honma ,  Takashi Kenmoku

IPC分类号： G03G900

CPC分类号： C08G63/6882 ,  C12P7/62 ,  G03G9/08755 ,  G03G9/08791 ,  Y10S435/874 ,  Y10S435/877

摘要： A polyhydroxyalkanoate characterized by having in the molecule a unit represented by Chemical Formula (1): wherein n may assume any one integral value within the range of from 1 to 8. Also disclosed are a process for producing the polyhydroxyalkanoate by the use of a microorganism having the ability to produce the polyhydroxyalkanoate and accumulate it in the bacterial body; a charge control agent, a toner binder and a toner which contain this polyhydroxyalkanoate; and an image-forming method and an image-forming apparatus which make use of the toner.

摘要翻译： 特征在于在分子中具有由化学式（1）表示的单元的聚羟基链烷酸酯：其中n可以在1至8的范围内呈现任何一个整数值。还公开了通过使用微生物制备聚羟基链烷酸酯的方法 具有生产聚羟基链烷酸酯并将其积聚在细菌体内的能力; 电荷控制剂，调色剂粘合剂和含有该聚羟基链烷酸酯的调色剂; 以及利用调色剂的图像形成方法和图像形成装置。

公开(公告)号：US07078200B2

公开(公告)日：2006-07-18

申请号：US10603996

申请日：2003-06-26

申请人： Tsutomu Honma ,  Etsuko Sugawa ,  Tetsuya Yano ,  Takeshi Imamura ,  Takashi Kenmoku

发明人： Tsutomu Honma ,  Etsuko Sugawa ,  Tetsuya Yano ,  Takeshi Imamura ,  Takashi Kenmoku

IPC分类号： C12P7/62 ,  C08G63/06 ,  C08G63/78

CPC分类号： C12P7/625 ,  C08G63/06 ,  C08G63/6822 ,  C08G63/6852

摘要： A method for producing a polyhydroxyalkanoate using a microorganism capable of substantially reducing unintended monomer units and obtaining the polyhydroxyalkanoate in a high yield. A microorganism capable of synthesizing a novel polyhydroxyalkanoate having 3-hydroxy-substituted benzoylalkanoic acid as a monomer unit, using a substituted benzoylalkanoic acid as a material, is cultured in a medium containing a substituted benzoylalkanoic acid. Then, the polyhydroxyalkanoate produced in the cultured bacteria is extracted and recovered.

摘要翻译： 使用能够显着降低非预期的单体单元并以高产率获得聚羟基链烷酸酯的微生物制备聚羟基链烷酸酯的方法。 在含有取代的苯甲酰基链烷酸的培养基中培养能够以取代的苯甲酰基链烷酸为原料合成具有3-羟基取代的苯甲酰基链烷酸作为单体单元的新型聚羟基链烷酸酯的微生物。 然后，提取并回收在培养细菌中产生的聚羟基链烷酸酯。

公开(公告)号：US06635782B2

公开(公告)日：2003-10-21

申请号：US09951720

申请日：2001-09-14

申请人： Tsutomu Honma ,  Etsuko Sugawa ,  Tetsuya Yano ,  Takeshi Imamura ,  Takashi Kenmoku

发明人： Tsutomu Honma ,  Etsuko Sugawa ,  Tetsuya Yano ,  Takeshi Imamura ,  Takashi Kenmoku

IPC分类号： C07C6976

CPC分类号： C12P7/625 ,  C08G63/06 ,  C08G63/6822 ,  C08G63/6852

摘要： To provide a novel polyhydroxyalkanoate and a manufacturing method by a microorganism capable of substantially reducing unintended monomer units and obtaining the polyhydroxyalkanoate in a high yield. A microorganism capable of synthesizing a novel polyhydroxyalkanoate having 3-hydroxy-substituted benzoylalkanoic acid as a monomer unit using a substituted benzoylalkanoic acid as a material is cultured in the medium containing a substituted benzoylalkanoic acid, then the polyhydroxyalkanoate produced in the cultured bacteria is extracted and recovered.

摘要翻译： 提供一种新型的聚羟基链烷酸酯及其制造方法，该微生物能够基本上减少非预期的单体单元并以高产率获得聚羟基链烷酸酯。 在含有取代的苯甲酰基链烷酸的培养基中培养能够使用取代的苯甲酰基链烷酸合成具有3-羟基取代的苯甲酰基链烷酸作为单体单元的新型聚羟基链烷酸酯的微生物，然后提取培养细菌中产生的聚羟基链烷酸酯， 恢复。

公开(公告)号：US07135540B2

公开(公告)日：2006-11-14

申请号：US10359600

申请日：2003-02-07

申请人： Tsutomu Honma ,  Shinya Kozaki ,  Takeshi Imamura ,  Takashi Kenmoku ,  Tatsuki Fukui ,  Etsuko Sugawa ,  Tetsuya Yano

发明人： Tsutomu Honma ,  Shinya Kozaki ,  Takeshi Imamura ,  Takashi Kenmoku ,  Tatsuki Fukui ,  Etsuko Sugawa ,  Tetsuya Yano

IPC分类号： C08G63/02

CPC分类号： C12P7/625 ,  C08G63/6822 ,  C08G63/6882 ,  C12P11/00

摘要： The invention provides a polyhydroxyalkanoate having a bromo group in a unit and being thermally stable and capable of arbitrarily controlling physical properties, and a producing method thereof. According to the invention, there are provided a polyhydroxyalkanoate copolymer including a 3-hydroxy-ω-bromoalkanoic acid unit represented by a formula—[OCH((CH2)nBr)CH2C(O)]— (n=1 to 8) (1) and a unit represented by a formula —[OCH((CH2)mR)CH2C(O)]— (2) within a same molecule, and a method for producing a polyhydroxyalkanoate copolymer by microorganisms, utilizing a ω-bromoalkanoic acid represented by a formula Br(CH2)pCH2CH2COOH (p=1 to 8) (20) and a compound represented by a formula R(CH2)qCH2CH2COOH (21) as raw materials.

摘要翻译： 本发明提供一种具有溴基团并且是热稳定的并能够任意控制物理性能的聚羟基链烷酸酯及其制备方法。 根据本发明，提供了一种包含由式 - [OCH（（CH 2）n）n Br表示的3-羟基-ω-溴代链烷酸单元的聚羟基链烷酸酯共聚物 ）CH 2 C（O）] - （n = 1-8）（1）和由式 - [OCH（（CH 2）2） （O）] - （2），以及通过微生物制备聚羟基链烷酸酯共聚物的方法，使用由以下化合物表示的ω-溴烷酸： 式（Br 2 CH 2 CH 2 CH 2 CH 2 CH 2 CH 2 CH 2 COOH（p = 1〜8）（20 ）和由式R（CH 2 CH 2）q CH 2 CH 2 COOH（21）表示的化合物 作为原料。

公开(公告)号：US07045321B2

公开(公告)日：2006-05-16

申请号：US10084172

申请日：2002-02-28

申请人： Takeshi Imamura ,  Etsuko Sugawa ,  Tetsuya Yano ,  Tsuyoshi Nomoto ,  Tomohiro Suzuki ,  Tsutomu Honma ,  Takashi Kenmoku ,  Tatsuki Fukui

发明人： Takeshi Imamura ,  Etsuko Sugawa ,  Tetsuya Yano ,  Tsuyoshi Nomoto ,  Tomohiro Suzuki ,  Tsutomu Honma ,  Takashi Kenmoku ,  Tatsuki Fukui

IPC分类号： C12P11/00 ,  C12P13/00 ,  C08G63/02

CPC分类号： G03G9/08755 ,  C08G63/6882 ,  C12P7/625 ,  C12P11/00 ,  G03G9/08791 ,  G03G9/09733 ,  G03G9/09775

摘要： A polyhydroxyalkanoate characterized by having in the molecule a unit represented by Chemical Formula (1): wherein n may assume any one integral value within the range of from 1 to 8. Also disclosed are a process for producing the polyhydroxyalkanoate by the use of a microorganism having the ability to produce the polyhydroxyalkanoate and accumulate it in the bacterial body; a charge control agent, a toner binder and a toner which contain this polyhydroxyalkanoate; and an image-forming method and an image-forming apparatus which make use of the toner.

摘要翻译： 公开了在分子中具有由化学式（1）表示的单元的聚羟基链烷酸酯。 其中R选自氢原子，卤原子，CN，NO 2，COOR'，SO 2 R“，CH 3，C 2 H 5，C 3 H 7，C（CH 3）2 H和C（CH 3）3; 其中R'是H，Na，K，CH3或C2H5，R“是OH，ONa，OK，卤素原子，OCH3或OC2H5; x是任意选自1至8的整数; 条件是排除了具有氢原子作为R的聚羟基链烷酸酯，并且所有单元中的x均为2或4.还公开了通过使用具有生产聚羟基链烷酸酯的能力的微生物来生产聚羟基链烷酸酯并累积 它在细菌体内

公开(公告)号：US06911521B2

公开(公告)日：2005-06-28

申请号：US10157142

申请日：2002-05-30

申请人： Takashi Kenmoku ,  Etsuko Sugawa ,  Tetsuya Yano ,  Tsuyoshi Nomoto ,  Takeshi Imamura ,  Tomohiro Suzuki ,  Tsutomu Honma

发明人： Takashi Kenmoku ,  Etsuko Sugawa ,  Tetsuya Yano ,  Tsuyoshi Nomoto ,  Takeshi Imamura ,  Tomohiro Suzuki ,  Tsutomu Honma

IPC分类号： C08G63/06 ,  C08G63/02 ,  C08G63/688 ,  C08G63/78 ,  C12P7/62 ,  C12P11/00

CPC分类号： C12P11/00 ,  C08G63/6882 ,  C12P7/625

摘要： A polyhydroxyalkanoate that comprises a unit represented by the following chemical formula (1): wherein R1 is a substituent of an aromatic ring selected from the group consisting of H, CH3, C2H5, CH3CH2CH2, (CH3)2CH, (CH3)3C, a halogen atom, CN, NO2, COOR′, and SO2R″, wherein R′ is selected from the group consisting of H, Na, K, CH3, and C2H5, and R″ is selected from the group consisting of OH, a halogen atom, ONa, OK, OCH3, and OC2H5; and x represents an integer of 1 to 8 being the same or different each other in the polyhydroxyalkanoate. A method for producing the polyhydroxyalkanoate is also provided.

摘要翻译： 一种聚羟基链烷酸酯，其包含由以下化学式（1）表示的单元：其中R1是选自H，CH 3，C 2， CH 3，CH 3，CH 2，CH 2，CH 3，CH 3，CH 3，CH 3， ）CH 2（CH 3）3 C，卤素原子，CN，NO 2，COOR' ，和SO 2 R“，其中R'选自H，Na，K，CH 3，和C 2 H 5，并且R“选自OH，卤素原子，ONa，OK，OCH 3和OC 2， 5 H 5; x表示在聚羟基链烷酸酯中相同或不同的1〜8的整数。 还提供了生产聚羟基链烷酸酯的方法。

公开(公告)号：US06808907B2

公开(公告)日：2004-10-26

申请号：US10105332

申请日：2002-03-26

申请人： Tsutomu Honma ,  Etsuko Sugawa ,  Tetsuya Yano ,  Tsuyoshi Nomoto ,  Takeshi Imamura ,  Tomohiro Suzuki ,  Takashi Kenmoku

发明人： Tsutomu Honma ,  Etsuko Sugawa ,  Tetsuya Yano ,  Tsuyoshi Nomoto ,  Takeshi Imamura ,  Tomohiro Suzuki ,  Takashi Kenmoku

IPC分类号： C12P762

CPC分类号： C12P7/625 ,  C12M47/06

摘要： The present invention provides a method for producing highly purified polyhydroxyalkanoate (PHA) with high yield, by removing cell components other than PHA from cells containing PHA. Moreover, the present invention provides a method for simply removing or reducing chlorine remained in the collected PHA particles, when a treatment with an oxidizing agent containing hypochlorite is performed in the production of PHA as described above. One of the above-described methods comprises a step of treating cells containing polyhydroxyalkanoate with an oxidizing agent containing at least hypochlorite, a step of separating the treated cells into a water-soluble fraction and a water-insoluble fraction, and a step of reducing chlorine remained in the water-insoluble fraction. The step of reducing chlorine may be a step of washing the water-insoluble fraction with a hot water, thiosulfate solution or polar solvent solution containing at least an organic polar solvent in which polyhydroxyalkanoate is insoluble.

摘要翻译： 本发明提供了通过从含有PHA的细胞中除去PHA以外的细胞成分而以高产率生产高纯度聚羟基链烷酸酯（PHA）的方法。 此外，本发明提供了如上所述在生产PHA中进行含有次氯酸盐的氧化剂的处理时，简单地除去或还原所收集的PHA颗粒中残留的氯的方法。 上述方法之一包括用至少含有次氯酸盐的氧化剂处理含有聚羟基链烷酸酯的细胞的步骤，将经处理的细胞分离成水溶性级分和水不溶性级分的步骤以及还原氯 保留在水不溶性部分中。 还原氯的步骤可以是用至少含有聚羟基链烷酸酯不溶的有机极性溶剂的热水，硫代硫酸盐溶液或极性溶剂溶液洗涤水不溶性部分的步骤。

公开(公告)号：US06645743B1

公开(公告)日：2003-11-11

申请号：US10372285

申请日：2003-02-25

申请人： Tsutomu Honma ,  Etsuko Sugawa ,  Tetsuya Yano ,  Takeshi Imamura ,  Takashi Kenmoku ,  Tatsuki Fukui

发明人： Tsutomu Honma ,  Etsuko Sugawa ,  Tetsuya Yano ,  Takeshi Imamura ,  Takashi Kenmoku ,  Tatsuki Fukui

IPC分类号： C12P742

CPC分类号： C08G63/06 ,  C08G63/6882 ,  C12P7/625 ,  C12P11/00 ,  Y10S435/874 ,  Y10S435/877

摘要： A polyhydroxyalkanoate (PHA) having a desired configuration is produced using a raw material containing &ohgr;-(4-vinylphenyl)-alkanoic acid and (&ohgr;-substituted alkanoic acid in which a group having a ring structure selected from phenyl, thienyl, and cyclohexyl structures substitutes therefor on the end thereof by producing a PHA copolymer containing the corresponding 3-hydroxy-&ohgr;-(4-vinylphenyl)-alkanoate unit and the corresponding 3-hydroxy-&ohgr;-substituted alkanoate unit by making use of a microorganism capable of producing the PHA or by oxidizing a predetermined portion of the corresponding PHA.

摘要翻译： 使用含有ω-（4-乙烯基苯基） - 链烷酸的原料和（其中具有选自苯基，噻吩基和环己基结构的环结构的基团的（ω-取代的链烷酸）制备具有所需构型的聚羟基链烷酸酯（PHA） 通过制备含有相应的3-羟基-ω-（4-乙烯基苯基） - 烷酸酯单元和相应的3-羟基-ω-取代的链烷酸酯单元的PHA共聚物，通过使用能够产生 或通过氧化相应PHA的预定部分。