公开(公告)号：US20230287402A1

公开(公告)日：2023-09-14

申请号：US18040684

申请日：2021-08-23

Applicant: University of Washington ,  MEDICAL RESEARCH COUNCIL, AS PART OF UNITED KINGDOM RESEARCH AND INNOVATION

Inventor： Ariel Ben SASSON ,  David BAKER ,  William SHEFFLER ,  Hannele RUOHOLA-BAKER ,  Logeshwaran SOMASUNDARM ,  Emmanuel DERIVERY ,  Joseph WATSON

IPC: C12N15/11 ,  C12N15/63

CPC classification number: C12N15/11 ,  C12N15/63

Abstract: The disclosure provides two-dimensional protein structures including first and second polypeptides that are different, each form homo-oligomers, and interact to form a rigid interface, polypeptide components of such two-dimensional protein structures, and uses thereof.

公开(公告)号：US20230130764A1

公开(公告)日：2023-04-27

申请号：US17931704

申请日：2022-09-13

Applicant: University of Washington ,  The Regents of the University of California

Inventor： Ingrid Swanson PULTZ ,  Clancey WOLF ,  Justin Bloomfield SIEGEL ,  Christine Elaine TINBERG ,  Lance STEWART ,  David BAKER

IPC: C12N9/50 ,  C12N9/52 ,  A61P1/14

Abstract: Polypeptides, and methods for their use, are disclosed that have an amino acid sequence at least 75% identical to the amino acid sequence of SEQ ID NO:1, are provided, wherein (a) the polypeptide degrades a PFQPQLPY (SEQ ID NO: 140) peptide and/or a PFPQPQQPF (SEQ ID NO: 68) at pH 4; (b) residue 467 is Ser, residue 267 is Glu, and residue 271 is Asp; and (c) the polypeptide comprises an amino acid change from SEQ ID NO: 1 at one or more residues selected from the group consisting of 221, 262E, 268, 269, 270, 319A, 320, 354E/Q/R/Y, 358S/Q/T, 368F/Q, 399, 402, 406, 424, 449, 461, 463, 105, 171, 172, 173, 174, and 456.

公开(公告)号：US20220017588A1

公开(公告)日：2022-01-20

申请号：US17294807

申请日：2019-11-19

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Alfredo QUIJANO RUBIO ,  Daniel Adriano SILVA MANZANO ,  David BAKER ,  Umut ULGE ,  Marc Joseph LAJOIE

IPC: C07K14/55 ,  C07K14/54 ,  A61P35/00 ,  A61K38/20 ,  A61P37/04

Abstract: Conditionally active receptor agonists that, when activated, bind to IL-2 receptor βc heterodimer (IL-2Rβc), IL-4 receptor αc heterodimer (IL-4Rαc), or IL-13 receptor a subunit (IL˜13 Rα) are disclosed, as are components of the conditionally active receptor agonists and methods for using the conditionally active receptor agonists.

公开(公告)号：US20210355175A1

公开(公告)日：2021-11-18

申请号：US17285033

申请日：2019-11-04

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Zibo CHEN ,  Scott BOYKEN ,  Sherry BERMEO ,  Robert A. LANGAN ,  David BAKER

IPC: C07K14/435 ,  G01N33/53

Abstract: Disclosed herein are designed heterodimer proteins, monomeric polypeptides capable of forming heterodimer proteins, protein scaffolds including such polypeptides, and methods for using the heterodimer proteins and subunit polypeptides for designing logic gates.

公开(公告)号：US20210284695A1

公开(公告)日：2021-09-16

申请号：US16489044

申请日：2018-04-27

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Gabriel Jacob ROCKLIN ,  David BAKER

IPC: C07K14/00 ,  C12N15/10

Abstract: Non-naturally occurring polypeptides are disclosed that include (a) 3-3 secondary structure elements, wherein each secondary structure element is either an α-helix (H domain) of between 10-20 amino acid residues in length or a β-strand (E domain) of between 3-10 amino acid residues in length; and (b) 2-4 linkers of between 2 to 6 amino acid residues in length connecting adjacent secondary structure elements; wherein the polypeptide is between 25-50 amino acid residues in length; and wherein the polypeptide includes no cysteine residues.

公开(公告)号：US20210171928A1

公开(公告)日：2021-06-10

申请号：US17019497

申请日：2020-09-14

Applicant: UNIVERSITY OF WASHINGTON ,  The Regents of the University of California

Inventor： Ingrid Swanson PULTZ ,  Clancey WOLF ,  Justin Bloomfield SIEGEL ,  Christine Elaine TINBERG ,  Lance STEWART ,  David BAKER

IPC: C12N9/50 ,  C12N9/52 ,  A61P1/14

Abstract: Polypeptides, and methods for their use, are disclosed that have an amino acid sequence at least 75% identical to the amino acid sequence of SEQ ID NO:1, are provided, wherein (a) the polypeptide degrades a PFQFQLPY (SEQ ID NO: 140) peptide and/or a PFPQPQQPF (SEQ ID NO: 68) at pH 4; (b) residue 467 is Ser, residue 267 is Glu, and residue 271 is Asp; and (c) the polypeptide comprises an amino acid change from SEQ ID NO: 1 at one or more residues selected from the group consisting of 221, 262E, 268, 269, 270, 319A, 320, 354E/Q/R/Y, 358S/Q/T, 368F/Q, 399, 402, 406, 424, 449, 461, 463, 105, 171, 172, 173, 174, and 456.

公开(公告)号：US20210054365A1

公开(公告)日：2021-02-25

申请号：US17091847

申请日：2020-11-06

Applicant: University of Washington

Inventor： David BAKER ,  David YOUNGER ,  Eric KLAVINS

IPC: C12N15/10

Abstract: The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.

公开(公告)号：US20200239524A1

公开(公告)日：2020-07-30

申请号：US16796009

申请日：2020-02-20

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Robert A. LANGAN ,  Scott BOYKEN ,  David BAKER ,  Walter NOVAK ,  Marc Joseph LAJOIE ,  Alfredo QUIJANO RUBIO

IPC: C07K14/00 ,  A61K47/69

Abstract: Disclosed are protein switches that can sequester bioactive peptides and/or binding domains, holding them in an inactive (“off”) state, until combined with a second designed polypeptide called the key, which induces a conformational change that activates (“on”) the bioactive peptide or binding domain, components of such protein switches, and their use.

公开(公告)号：US20200017552A1

公开(公告)日：2020-01-16

申请号：US16483865

申请日：2018-03-15

Applicant: UNIVERSITY OF WASHINGTON

Inventor： David BAKER ,  Aaron Arthur CHEVALIER ,  Gabriel Jacob ROCKLIN ,  Christopher David BAHL ,  Lance Joseph STEWART ,  Daniel Adriano SILVA MANZANO ,  Deborah L. FULLER ,  Merika TREANTS KODAY ,  Jason GILMORE

IPC: C07K14/00 ,  G01N33/569 ,  C07K16/00 ,  A61P31/16

Abstract: Polypeptides that bind to viral hemagglotinin are disclosed and methods for their use in treating or limiting influenza infection, and diagnosing influenza infection.

公开(公告)号：US20200002398A1

公开(公告)日：2020-01-02

申请号：US16572038

申请日：2019-09-16

Applicant: UNIVERSITY OF WASHINGTON ,  THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY

Inventor： Daniel Adriano SILVA MANZANO ,  Shawn YU ,  Umut ULGE ,  David BAKER ,  Kenan Christopher GARCIA ,  Jamie SPANGLER ,  Carl WALKEY ,  Alfredo QUIJANO RUBIO

IPC: C07K14/55 ,  C07K14/54

Abstract: De novo designed polypeptides that bind to IL-2 receptor βγcheterodimer (IL-2Rβγc), IL-4 receptor αγcheterodimer (IL-4Rαγc), or IL-13 receptor α subunit (IL-13Rα) are disclosed, as are methods for using and designing the polypeptides.