公开(公告)号：US20210380641A1

公开(公告)日：2021-12-09

申请号：US16762565

申请日：2018-11-09

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Gabriel BUTTERFIELD ,  Marc Joseph LAJOIE ,  Neil P. KING ,  David BAKER ,  Daniel ELLIS

IPC: C07K14/00 ,  C12N15/87 ,  B82Y40/00

Abstract: Synthetic nanostructures, polypeptides that are useful, for example, in making synthetic nanostructures, and methods for using synthetic nanostructures are disclosed herein.

公开(公告)号：US20210115090A1

公开(公告)日：2021-04-22

申请号：US17077692

申请日：2020-10-22

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Robert A. LANGAN ,  Scott BOYKEN ,  David BAKER ,  Walter NOVAK ,  Marc Joseph LAJOIE ,  Alfredo QUIJANO RUBIO

IPC: C07K14/00 ,  A61K47/69

Abstract: Disclosed are protein switches that can sequester bioactive peptides and/or binding domains, holding them in an inactive (“off”) state, until combined with a second designed polypeptide called the key, which induces a conformational change that activates (“on”) the bioactive peptide or binding domain, components of such protein switches, and their use.

公开(公告)号：US20220226485A1

公开(公告)日：2022-07-21

申请号：US17618117

申请日：2020-06-17

Applicant: University of Washington

Inventor： Nicholas WOODALL ,  Scott BOYKEN ,  Marc Joseph LAJOIE ,  Zibo CHEN ,  Robert A. LANGAN ,  David BAKER

IPC: A61K47/64 ,  C07K14/00 ,  C12N15/62 ,  C12N15/85

Abstract: The present disclosure provides a chimeric polypeptide comprising a helical bundle which comprises between about two and about seven alpha-helices and a bioactive peptide, wherein one or more of the alpha helices form one or more inter-helix hydrogen bonds and comprise at least one phosphorylation site and wherein the bioactive peptide is conformationally placed inside the helical bundle so that the bioactive peptide is not activated or exposed. The disclosure also provides a nucleotide sequence encoding the chimeric polypeptide, a vector comprising the nucleotide sequence, a cell comprising the nucleotide sequence, and method of making, using, or designing the chimeric polypeptide.

公开(公告)号：US20220218752A1

公开(公告)日：2022-07-14

申请号：US17610083

申请日：2020-05-18

Applicant: University of Washington ,  FRED HUTCHINSON CANCER RESEARCH CENTER

Inventor： David BAKER ,  Scott BOYKEN ,  Marc Joseph LAJOIE ,  Robert A. LANGAN ,  Stanley R. RIDDELL ,  Alexander SALTER

IPC: A61K35/17 ,  C12N15/86 ,  C12N5/0783 ,  C12N15/62 ,  A61K38/17 ,  C07K14/47 ,  C07K14/725 ,  C07K14/705 ,  C07K14/005

Abstract: Disclosed are protein switches that can sequester bioactive peptides and/or binding domains, holding them in an inactive (“off”) state, until combined with a second designed polypeptide called the key, which induces a conformational change that activates (“on”) the bioactive peptide or binding domain only when the protein switch components are co/localized when bound to their targets, components of such protein switches, and their use.

公开(公告)号：US20220017588A1

公开(公告)日：2022-01-20

申请号：US17294807

申请日：2019-11-19

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Alfredo QUIJANO RUBIO ,  Daniel Adriano SILVA MANZANO ,  David BAKER ,  Umut ULGE ,  Marc Joseph LAJOIE

IPC: C07K14/55 ,  C07K14/54 ,  A61P35/00 ,  A61K38/20 ,  A61P37/04

Abstract: Conditionally active receptor agonists that, when activated, bind to IL-2 receptor βc heterodimer (IL-2Rβc), IL-4 receptor αc heterodimer (IL-4Rαc), or IL-13 receptor a subunit (IL˜13 Rα) are disclosed, as are components of the conditionally active receptor agonists and methods for using the conditionally active receptor agonists.

公开(公告)号：US20200239524A1

公开(公告)日：2020-07-30

申请号：US16796009

申请日：2020-02-20

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Robert A. LANGAN ,  Scott BOYKEN ,  David BAKER ,  Walter NOVAK ,  Marc Joseph LAJOIE ,  Alfredo QUIJANO RUBIO

IPC: C07K14/00 ,  A61K47/69

Abstract: Disclosed are protein switches that can sequester bioactive peptides and/or binding domains, holding them in an inactive (“off”) state, until combined with a second designed polypeptide called the key, which induces a conformational change that activates (“on”) the bioactive peptide or binding domain, components of such protein switches, and their use.

公开(公告)号：US20190352639A1

公开(公告)日：2019-11-21

申请号：US16476917

申请日：2018-02-12

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Marc Joseph LAJOIE ,  Elizabeth Erin GRAY ,  Neil P. KING ,  Daniel Brewster STETSON ,  David BAKER ,  Katherine DaPRON

IPC: C12N15/11 ,  C12N9/22 ,  C12N15/85 ,  C07K14/005 ,  C12N7/00

Abstract: The application discloses multimeric assemblies packaging one or more active component and their use to carry out nucleic acid regulation or gene editing.

公开(公告)号：US20220273711A1

公开(公告)日：2022-09-01

申请号：US17609972

申请日：2020-05-18

Applicant: University of Washington

Inventor： Scott BOYKEN ,  Marc Joseph LAJOIE ,  Robert A. LANGAN ,  David BAKER ,  Jilliane Ruth BRUFFEY

IPC: A61K35/17 ,  C12N15/62 ,  C07K16/18

Abstract: Disclosed am protein switches that can sequester bioactive peptides and/or binding domains, holding them in an inactive (“off”) state, until combined with a second designed polypeptide called die key, which induces a conformational change that activates (“on”) the bioactive peptide or binding domain only when the protein switch components are co-localized when bound to their targets, components of such protein switches, and their use.

公开(公告)号：US20180320166A1

公开(公告)日：2018-11-08

申请号：US15765045

申请日：2016-10-01

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Marc Joseph LAJOIE ,  Jason Chesler KLEIN ,  Jerrod Joseph SCHWARTZ ,  David BAKER ,  Jay Ashok SHENDURE ,  Lance Joseph STEWART

IPC: C12N15/10

CPC classification number: C12N15/1031 ,  C12N15/10 ,  C12P19/34

Abstract: The present invention provides methods for multiplex assembly of oligonucleotides.