中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

89 records (took 0.026 seconds)

    HIGH THROUGHPUT PROTEIN-PROTEIN INTERACTION SCREENING IN YEAST LIQUID CULTURE
    1.
    发明公开
    HIGH THROUGHPUT PROTEIN-PROTEIN INTERACTION SCREENING IN YEAST LIQUID CULTURE 审中-公开

    公开(公告)号：US20240263167A1

    公开(公告)日：2024-08-08

    申请号：US18484871

    申请日：2023-10-11

    Applicant: University of Washington

    Inventor： David BAKER ,  David YOUNGER ,  Eric KLAVINS

    IPC: C12N15/10

    CPC classification number: C12N15/1055

    Abstract: The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.

    Repeat Protein Architectures
    3.
    发明公开
    Repeat Protein Architectures 审中-公开

    公开(公告)号：US20230272446A1

    公开(公告)日：2023-08-31

    申请号：US18312788

    申请日：2023-05-05

    Applicant: University of Washington

    Inventor： Fabio PARMEGGIANI ,  TJ BRUNETTE ,  Po-Ssu HUANG ,  David BAKER

    IPC: C12P21/02 ,  C07K14/00 ,  C12N15/70

    CPC classification number: C12P21/02 ,  C07K14/001 ,  C12N15/70 ,  C12N2800/101

    Abstract: Methods and systems for designing proteins are disclosed, as well as proteins and protein assemblies designed. A computing device can determine a protein repeating unit that includes one or more protein helices and one or more protein loops. The computing device can generate a protein backbone structure with a copy of the protein repeating unit. The computing device can determine whether a distance between a pair of helices of the protein backbone structure is between lower and upper distance thresholds. After determining that the distance between the pair of helices is between the lower and upper distance thresholds, the computing device can: generate a plurality of protein sequences based on the protein backbone structure, select a particular protein sequence of the plurality of protein sequences based on an energy landscape that has information about energy and distance from a target fold of the particular protein sequence, and generate an output based on the particular protein sequence.

    SELF-ASSEMBLING PROTEIN HOMO-POLYMERS
    8.
    发明申请
    SELF-ASSEMBLING PROTEIN HOMO-POLYMERS 有权

    公开(公告)号：US20210324011A1

    公开(公告)日：2021-10-21

    申请号：US17285057

    申请日：2019-10-24

    Applicant: UNIVERSITY OF WASHINGTON

    Inventor： Hao SHEN ,  Jorge FALLAS ,  David BAKER

    IPC: C07K14/00 ,  C07K1/00

    Abstract: Disclosed herein are polypeptides having the amino acid sequence that is at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to the full length of the amino acid sequence selected from the group consisting of SEQ ID NO: 1-33 and 36, wherein the polypeptides include at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% of the identified interface residues, and wherein the polypeptides are capable of end-to-end homo-polymerization, homo-polymers of the polypeptides, and related capping and anchor proteins to facilitate homo-polymer formation.

Patent Agency Ranking