公开(公告)号：US11061039B2

公开(公告)日：2021-07-13

申请号：US16208739

申请日：2018-12-04

Applicant: CLEVELAND HEARTLAB, INC.

Inventor： Timothy Collier ,  Cory Bystrom ,  Angela Higgins

IPC: G01N33/483 ,  G01N33/92 ,  G01N1/34 ,  C07K14/775

Abstract: The present invention provides methods, kits, and compositions for purifying HDL molecules from a sample (e.g., blood sample) using HDL tagging molecules comprising an HDL lipophilic core binding peptide (e.g., portion of ApoA1) and an affinity tag. The present invention also provides methods, kits, and compositions for detecting non-fragmented ApoA1 with mass spectrometry. The present invention further provides methods, kits, and compositions for tagging HDL molecules in a sample with detectably labeled ApoA1 molecules such that the ratio of detectably labeled ApoA1 molecules to native ApoA1 proteins may be determined.

公开(公告)号：US10921331B2

公开(公告)日：2021-02-16

申请号：US15656592

申请日：2017-07-21

Applicant: Cleveland HeartLab, Inc.

Inventor： Cory Bystrom ,  Timothy Collier

IPC: G01N33/92

Abstract: Provided herein are methods, systems, and compositions for detecting one or more HDL-associated proteins (e.g., ApoC3; ApoC3 and ApoA1; ApoC3 and SAA1/2; or proteins in Biomarker Panels 1-30) in a sample from a subject with, or suspected of having, cardiovascular disease (CVD) or other HDL related disease. In certain embodiments, such methods, systems, and compositions are used to determine the approximate risk of CVD (or other disease) for a subject, and/or the approximate cholesterol efflux capacity (CEC) of a sample.

公开(公告)号：US10466260B2

公开(公告)日：2019-11-05

申请号：US15788876

申请日：2017-10-20

Applicant: Cleveland HeartLab, Inc.

Inventor： Cory Bystrom ,  Timothy Collier

IPC: A61K38/00 ,  G01N33/92

Abstract: Provided herein are compositions, systems, and methods for extracting and detecting at least one HDL-associated protein (e.g., ApoA1) from a sample (e.g., plasma or serum sample). In certain embodiments, a strong organic acid and hydrophilic organic solvent are mixed with the sample; after centrifugation, the supernatant is transferred to a second container where it is mixed with a non-polar organic solvent; after centrifugation, the lower aqueous layer is transferred to a third container; and then at least a portion of the transferred aqueous layer is subjected to a detection assay such that at least one HDL-associated protein is detected.

公开(公告)号：US20090118381A1

公开(公告)日：2009-05-07

申请号：US11950192

申请日：2007-12-04

Applicant: George Russell Warnick ,  Gary Hewett

Inventor： George Russell Warnick ,  Gary Hewett

IPC: C07K1/18 ,  C08J5/20

CPC classification number: B01J20/26 ,  B01J20/261 ,  B01J20/264 ,  B01J20/267 ,  B01J20/28042 ,  B01J20/285 ,  B01J2220/82

Abstract: Described are polymer monolith compositions for separating high density lipoprotein, as well as related methods of use.

Abstract translation: 描述了用于分离高密度脂蛋白的聚合物整料组合物以及相关的使用方法。

公开(公告)号：US07257832B2

公开(公告)日：2007-08-14

申请号：US09974406

申请日：2001-10-09

Applicant: John A. Beane ,  Larry J. Heminger ,  Robert M. Stone

Inventor： John A. Beane ,  Larry J. Heminger ,  Robert M. Stone

IPC: H04N7/173

CPC classification number: G06F19/321 ,  G06F19/00 ,  G06F19/3418 ,  G06Q50/24

Abstract: As patient video images are captured in a lab, they are converted into an uncompressed data set and stored locally on a hospital site server, where they are immediately viewable by diagnosticians in the hospital. The hospital site server generates a plurality of compressed data sets for use by the Internet Data Center. Additionally, the uncompressed data set and a plurality of compressed data sets are stored permanently on a centralized Internet Data Center, from which they can be searched out and displayed by any client device running web-browser software. A client is provided with immediate access to the uncompressed images when pausing and requesting the images of interest from the server. The patient video images are automatically delivered to any authorized Clinical Research Organizations, they are delivered back to the treating hospital when the patient returns for subsequent visits, and are viewable through in-hospital viewing stations over a private high-speed network.

Abstract translation: 由于患者的视频图像在实验室中被捕获，所以将它们转换为未压缩的数据集，并将其存储在医院现场服务器上，并立即由医院诊断人员查看。 医院站点服务器生成多个压缩数据集供互联网数据中心使用。 此外，未压缩数据集和多个压缩数据集被永久地存储在集中式互联网数据中心上，由运行网络浏览器软件的任何客户端设备可从中搜索出并显示。 当客户端暂停并请求服务器感兴趣的图像时，可以立即访问未压缩映像。 患者视频图像自动传送到任何授权的临床研究组织，当患者返回后续访问时，他们被送回治疗医院，并可通过私人高速网络通过医院内观察站查看。

公开(公告)号：US07226792B2

公开(公告)日：2007-06-05

申请号：US10854402

申请日：2004-05-26

Applicant: Amy C. Roberts ,  David T. Shewmake

Inventor： Amy C. Roberts ,  David T. Shewmake

IPC: G01N33/92

CPC classification number: G06Q10/10 ,  G01N33/92 ,  G01N2800/044 ,  G06F19/00 ,  G06Q50/24

Abstract: The invention provides novel methods for selecting an optimal diet and exercise regimen for a patient based on the consideration of several factors, including low density lipoprotein (LDL) and high density lipoprotein (HDL) subclass levels. Furthermore, the invention provides novel methods for treating a patient with cardiovascular disease (CVD) or at risk of developing CVD through selection of an optimal diet and exercise regimen, based on the measurement of risk factors, including LDL and HDL subclass levels.

Abstract translation: 本发明提供了基于对低密度脂蛋白（LDL）和高密度脂蛋白（HDL）亚类水平的几个因素的考虑，为患者选择最佳饮食和运动方案的新方法。 此外，本发明基于包括LDL和HDL亚类水平在内的风险因素的测量，提供了通过选择最佳饮食和运动方案来治疗患有心血管疾病（CVD）或有发展CVD风险的新型方法。

公开(公告)号：US20070068539A1

公开(公告)日：2007-03-29

申请号：US11522650

申请日：2006-09-18

Applicant: Christopher Hall ,  Vance Lanier ,  David Shewmake ,  Frank Ruderman ,  Matthew Banet ,  Randon Schultz ,  Marshal Dhillon ,  Adam Fleming ,  Henk Visser

Inventor： Christopher Hall ,  Vance Lanier ,  David Shewmake ,  Frank Ruderman ,  Matthew Banet ,  Randon Schultz ,  Marshal Dhillon ,  Adam Fleming ,  Henk Visser

IPC: G06F19/00

CPC classification number: A61B5/0022 ,  A61B5/021 ,  A61B5/024 ,  A61B5/145 ,  A61B5/14532 ,  G06F19/3481 ,  G06Q50/22 ,  G16H10/20 ,  G16H10/60 ,  G16H15/00

Abstract: The invention provides a system that determines if a patient has Metabolic Syndrome, and in response provides a disease-management program that helps reduce medical risks associated with this malady. The system features a device configured to collect glucose information and blood pressure information, and then transmit this information to a central computer system. The system also includes a database configured to receive triglyceride information and cholesterol information from an external blood test, and a central computer system featuring: 1) a communication interface configured to communicate with the device to receive glucose and blood pressure information and with the database to receive triglyceride and cholesterol information; 2) a user interface configured to accept patient information; and 3) a processor configured to operate an algorithm that processes the glucose, blood pressure, triglyceride, cholesterol, and patient information to determine if the patient has Metabolic Syndrome.

Abstract translation: 本发明提供了一种确定患者是否具有代谢综合征的系统，并且作为响应提供了有助于减少与该疾病相关的医疗风险的疾病管理程序。 该系统具有被配置为收集葡萄糖信息和血压信息的设备，然后将该信息发送到中央计算机系统。 该系统还包括配置为从外部血液测试中接收甘油三酯信息和胆固醇信息的数据库，以及中央计算机系统，其特征在于：1）通信接口，被配置为与设备通信以接收葡萄糖和血压信息，并且与数据库 接受甘油三酯和胆固醇信息; 2）被配置为接收患者信息的用户界面; 以及3）处理器，其被配置为操作处理葡萄糖，血压，甘油三酯，胆固醇和患者信息以确定患者是否具有代谢综合征的算法。

公开(公告)号：US20060161066A1

公开(公告)日：2006-07-20

申请号：US11335860

申请日：2006-01-18

Applicant: Jonathan Elion

Inventor： Jonathan Elion

IPC: A61B5/04

CPC classification number: A61B5/044 ,  A61B5/0452 ,  A61B5/7435 ,  A61B5/7445

Abstract: A method and system for generating electrocardiogram reports allows for the editing of features in the electrocardiogram interpretation process. This improves the accuracy of machine interpretation of the ECG data thereby facilitating the analysis and generation of the final report by the physician. The present method and system are most useful in host-based ECG interpretation systems where the physician accesses the ECG data at a workstation including a machine interpretation that is generated typically at the ECG cart. The physician is then provided with the ability to modify the features in the ECG data and generate a new host-based interpretation based on the original ECG data and the features specified by the physician.

Abstract translation: 用于产生心电图报告的方法和系统允许编辑心电图解释过程中的特征。 这提高了ECG数据的机器解释的准确性，从而便于医生分析和生成最终报告。 本方法和系统在基于主机的ECG解释系统中是最有用的，其中医师在工作站处访问ECG数据，包括通常在ECG车上产生的机器解释。 然后向医师提供修改ECG数据中的特征并基于原始ECG数据和由医师指定的特征产生新的基于主机的解释的能力。

公开(公告)号：US20050042761A1

公开(公告)日：2005-02-24

申请号：US10959656

申请日：2004-10-06

Applicant: David Shewmake ,  Frank Ruderman ,  Christopher Boggess

Inventor： David Shewmake ,  Frank Ruderman ,  Christopher Boggess

IPC: G01N33/92

CPC classification number: G01N33/92 ,  G01N2800/32 ,  G01N2800/52

Abstract: The invention provides a method for identifying patients with normal NCEP lipid levels who are in need of treatment for cardiovascular disease comprising measuring one or more LDL or HDL particle subclass levels and identifying abnormal LDL or HDL subclass levels. LDL III a+b and HDL 2b are preferred subclasses.

Abstract translation: 本发明提供了一种用于鉴定需要治疗心血管疾病的正常NCEP脂质水平的患者的方法，包括测量一种或多种LDL或HDL颗粒亚类水平并鉴定异常的LDL或HDL亚类水平。 LDL III a + b和HDL 2b是优选的亚类。

公开(公告)号：US20030235918A1

公开(公告)日：2003-12-25

申请号：US10412838

申请日：2003-04-12

Applicant: Berkeley HeartLab, Inc.

Inventor： David Shewmake ,  Frank Ruderman ,  Christopher Boggess ,  Faith Clendenen

IPC: G01N031/00

CPC classification number: G01N33/92 ,  C25B1/285 ,  G01N2800/32 ,  G01N2800/52 ,  Y10T436/104165

Abstract: The invention provides a data base of LDL, I, Ia, IIb, IIIa, IIb, IVa, IVb and HDL2a, HDL2b, HDL 3a, HDL 3b and HDL 3c together with patient data such as HDL-C, LDL-C, Apo A, ApoB, Lp(a) and patient personal data useful for treatment, diagnosing, and monitoring cardiovascular disease. The data base contains the LDL and HDL subfraction data in quantitative mg/dl values and permits deriving relationship amongst the LDL and HDL values and cardiovascular disease. Quantitative data permits more effective treatment and monitoring of cardiovascular disease. For example, quantitative differences in LDL and HDL subclass levels can determine the need for more or less aggressive treatment. The data base which includes patient events, procedures, interventions which is correlated to LDL and HD1 quantitative subclass data permits development of personalized patient treatment plans and monitoring the effectiveness of such treatment. Thus, LDL and HDL quantitative subfraction data can be used to more effectively treat and monitor cardiovascular disease. The invention, for example, provides a method for identifying patients with normal NCEP lipid levels who are in need of treatment for cardiovascular disease comprising measuring one or more LDL or HDL particle subclass levels and identifying abnormal LDL or HDL subclass levels. LDL III a & b and HDL 2b are preferred subclasses.

Abstract translation: 本发明提供LDL，I，Ia，IIb，IIIa，IIb，IVa，IVb和HDL2a，HDL2b，HDL 3a，HDL 3b和HDL 3c的数据库以及患者数据如HDL-C，LDL-C，Apo A，ApoB，Lp（a）和患者个人资料可用于治疗，诊断和监测心血管疾病。 数据库包含定量mg / dl值中的LDL和HDL次级分数据，并允许LDL和HDL值与心血管疾病之间的关系。 定量数据可以更有效地治疗和监测心血管疾病。 例如，LDL和HDL亚类水平的定量差异可以确定对或多或少积极治疗的需要。 包括与LDL和HD1定量亚类数据相关的患者事件，程序，干预措施的数据库允许开发个性化的患者治疗计划并监测此类治疗的有效性。 因此，LDL和HDL定量分数据可用于更有效地治疗和监测心血管疾病。 例如，本发明提供了一种用于鉴定需要治疗心血管疾病的正常NCEP脂质水平的患者的方法，包括测量一种或多种LDL或HDL颗粒亚类水平并鉴定异常的LDL或HDL亚类水平。 LDL III a＆b和HDL 2b是优选的亚类。