公开(公告)号：US20090247425A1

公开(公告)日：2009-10-01

申请号：US12058531

申请日：2008-03-28

Applicant: Hua Zhang ,  Mark Andersen

Inventor： Hua Zhang ,  Mark Andersen

IPC: C40B40/06 ,  C40B50/00 ,  C12N9/24 ,  C12N9/12 ,  C12N9/16 ,  C40B50/14

CPC classification number: C12Q1/6837 ,  C12Q2521/301 ,  C12Q2521/531

Abstract: The present disclosure relates generally to compositions and methods for the reuse of arrays, including microarrays. Specifically, the present disclosure discloses polynucleotide targets comprising nucleotide analogs that are not present within the probe polynucleotides immobilized on the array. The nucleotide-analog containing targets can be chemically modified to reduce their thermal stability and thus easier to remove from the array. In preferred embodiments, the disclosure relates to DNA probes hybridized to single-stranded deoxyribouridine-containing targets, the targets subsequently being chemically modified using a uracil DNA glycosylase and/or nuclease. Accordingly, the disclosure allows for the glycosylase treated, deoxyuridine-containing targets to be removed from the array by exposure to less stringent denaturing conditions than otherwise would have been required. Using less stringent denaturing conditions permits reuse of the array by reducing damage to the probe polynucleotides immobilized on the array during target removal.

Abstract translation: 本公开一般涉及用于重新使用阵列（包括微阵列）的组合物和方法。 具体地，本公开公开了包含不存在于固定在阵列上的探针多核苷酸内的核苷酸类似物的多核苷酸靶。 可以化学修饰含核苷酸类似物的靶标，以降低其热稳定性，从而更容易从阵列中除去。 在优选的实施方案中，本公开涉及与单链脱氧核糖嘌呤的靶标杂交的DNA探针，随后使用尿嘧啶DNA糖基化酶和/或核酸酶化学修饰靶标。 因此，本公开允许经过糖基化酶处理的含脱氧尿苷的靶通过暴露于不同于本来需要的较不严格的变性条件下从阵列中除去。 使用较不严格的变性条件允许通过在目标去除期间减少固定在阵列上的探针多核苷酸的损伤来重新使用阵列。

公开(公告)号：US07544961B2

公开(公告)日：2009-06-09

申请号：US12135934

申请日：2008-06-09

Applicant: Philip T. Feldsine ,  Tim A. Kelly ,  Jim Christensen ,  Joseph B. Di Carlo ,  Mark Andersen ,  Anita Kressner

Inventor： Philip T. Feldsine ,  Tim A. Kelly ,  Jim Christensen ,  Joseph B. Di Carlo ,  Mark Andersen ,  Anita Kressner

IPC: G01N15/06

CPC classification number: G01N21/8507 ,  G01N21/645 ,  G01N21/76 ,  G01N21/763 ,  G01N2001/028

Abstract: Methods and apparatus for evaluating the quality of a sample of a product, an ingredient, an environment or process by measuring multiple parameters thereof, including light emitted from a reacting sample containing ATP, ADP, alkaline phosphatase or other parameters such as pH, temperature, conductivity, reduction potential, dissolved gases, specific ions, and microbiological count. The apparatus comprises an integrated sample testing device used to collect a sample, mix reagents, react the sample, and collect it in a measurement chamber. The apparatus also comprises an instrument having a photon detection assembly for use with the sample testing device. The instrument can also comprise one or more sensing probes and a communication port to facilitate data collection, transfer and analysis.

Abstract translation: 通过测量其多个参数，包括从含有ATP，ADP，碱性磷酸酶或其它参数如pH，温度的反应样品发射的光来评价产品，成分，环境或过程的样品的质量的方法和装置， 电导率，还原电位，溶解气体，特定离子和微生物计数。 该装置包括用于收集样品，混合试剂，使样品反应并将其收集在测量室中的集成样品测试装置。 该装置还包括具有用于样品测试装置的光子检测组件的仪器。 仪器还可以包括一个或多个感测探头和通信端口，以便于数据收集，传输和分析。

公开(公告)号：US20070026430A1

公开(公告)日：2007-02-01

申请号：US11428191

申请日：2006-06-30

Applicant: Mark Andersen ,  Benjamin Schroeder ,  Kai Lao

Inventor： Mark Andersen ,  Benjamin Schroeder ,  Kai Lao

IPC: C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC classification number: C12Q1/6813 ,  C12Q1/686 ,  C12Q2525/301 ,  C12Q2533/101 ,  C12Q2521/301

Abstract: The present teachings provide methods, compositions, and kits for detecting target analytes, including proteins. In some embodiments, cleavage reactions are performed in the context of proximity probe reactions that query target proteins, wherein the presence and/or quantity of cleavage products is indicative of the presence and/or quantity of a target protein. In some embodiments, the cleavage fragments are quantitated using a real time PCR assay comprising a stem-loop primer, wherein the stem-loop primer comprises a self-complementary hairpin structure and a free 3′ end end complementary to the cleavage product. In some embodiments, polymerase extension approaches are employed in the context of proximity probe reactions.

Abstract translation: 本教导提供了用于检测目标分析物（包括蛋白质）的方法，组合物和试剂盒。 在一些实施方案中，裂解反应在查询靶蛋白的邻近探针反应的上下文中进行，其中切割产物的存在和/或数量指示靶蛋白的存在和/或数量。 在一些实施方案中，使用包含茎环引物的实时PCR测定法定量切割片段，其中茎环引物包含自身互补的发夹结构和与切割产物互补的游离的3'末端。 在一些实施方案中，在邻近探针反应的上下文中使用聚合酶延伸方法。

公开(公告)号：US07030403B2

公开(公告)日：2006-04-18

申请号：US10313941

申请日：2002-12-05

Applicant: Philip T. Feldsine ,  Tim A. Kelly ,  Jim Christensen ,  Joseph B. Di Carlo ,  Mark Andersen ,  Anita Kressner

Inventor： Philip T. Feldsine ,  Tim A. Kelly ,  Jim Christensen ,  Joseph B. Di Carlo ,  Mark Andersen ,  Anita Kressner

IPC: G01N15/06

CPC classification number: G01N21/8507 ,  G01N21/645 ,  G01N21/76 ,  G01N21/763 ,  G01N2001/028

Abstract: Methods and apparatus for evaluating the quality of a sample of a product, an ingredient, an environment or process by measuring multiple parameters thereof, including light emitted from a reacting sample containing ATP, ADP, alkaline phosphatase or other parameters such as pH, temperature, conductivity, reduction potential, dissolved gases, specific ions, and microbiological count. The apparatus comprises an integrated sample testing device used to collect a sample, mix reagents, react the sample, and collect it in a measurement chamber. The apparatus also comprises an instrument having a photon detection assembly for use with the sample testing device. The instrument can also comprise one or more sensing probes and a communication port to facilitate data collection, transfer and analysis.

Abstract translation: 通过测量其多个参数，包括从含有ATP，ADP，碱性磷酸酶或其它参数如pH，温度的反应样品发射的光来评价产品，成分，环境或过程的样品的质量的方法和装置， 电导率，还原电位，溶解气体，特定离子和微生物计数。 该装置包括用于收集样品，混合试剂，使样品反应并将其收集在测量室中的集成样品测试装置。 该装置还包括具有用于样品测试装置的光子检测组件的仪器。 仪器还可以包括一个或多个感测探头和通信端口，以便于数据收集，传输和分析。

公开(公告)号：US06640503B1

公开(公告)日：2003-11-04

申请号：US09628598

申请日：2000-07-28

Applicant: Lawrence P. Evensen ,  Mark Andersen ,  Richard Spindel

Inventor： Lawrence P. Evensen ,  Mark Andersen ,  Richard Spindel

IPC: E04D338

CPC classification number: E04D13/1407 ,  E04F11/1812 ,  E04F11/1814

Abstract: A deck post (or brace) having a non-circular cross-section is secured to a roof deck. A lead pipe jack (sleeve) is slid over the post. A waterproofing assembly having a collar and a skirt is provided; the collar has an opening with generally the same non-circular cross-section as that of the post. The collar opening is positioned on the deck post, and the unit is slid down the post until the collar is at the top of the sleeve, the skirt extending down over the sleeve. A clamp is then positioned and tightened around the collar to provide a watertight seal on the deck post. When the post is already installed on a roof deck and the sleeve and the waterproofing assembly cannot be slid into place on the post, a modified retrofit waterproofing assembly and sleeve are used to provide the waterproofing. The modified sleeve is a split lead flashing jack with an open seam on one side. The sleeve is opened up, wrapped around the post and soldered closed. The modified waterproofing assembly has a split joint through the skirt and collar. To position this assembly on the post at the top of the split lead flashing jack, the joint is opened up, the assembly wrapped around the post and the slip joint closed with a watertight flap. A locking closure can be provided having a locking clasp and a locking slot. Another alternative waterproofing assembly forms the collar as a plug separate from the skirt; the skirt is opened and the plug inserted therein. This arrangement allows a plug having the desired opening configuration to be selected from an inventory of different plugs and used with a single skirt design. Another alternative of the waterproofing assembly provides a series of flanged tabs on the top edge of the collar for connecting to the collar plug. The flanged tabs may each have a projection on them which increases the pull-out resistance between a connected skirt and collar. The collar plug may also have a series of cavities which line up with the flanged tabs of the skirt. The collar plug may have a recess for the clamp. As an alternative assembly, multiple skirts can be connected together using their locking clasps and locking slots and the resulting larger skirt can then be connected to a collar.

公开(公告)号：US5632048A

公开(公告)日：1997-05-27

申请号：US531111

申请日：1995-09-20

Applicant: David Mortell ,  Mark Andersen ,  Lawrence A. Bernstein

Inventor： David Mortell ,  Mark Andersen ,  Lawrence A. Bernstein

IPC: A42B3/04 ,  A42B3/16 ,  A42B3/28 ,  A42B3/00

CPC classification number: A42B3/166

Abstract: A helmet for use by riders of bicycles, motorcycles or other vehicles. Left and right hearing assemblies are mounted inside of the helmet. Each of the assemblies has an enclosed ear cup positioned to fit snugly over a respective one of the user's ears. Respective hearing conduits extend from the ear cups through the interior of the helmet to respective openings. Thereby while the ear cups block substantial wind noises, the hearing conduits direct desirable sounds, such as emergency sirens, other traffic noises and voices, to the user's ears. User operable actuators for closing and opening the hearing conduits as desired can be provided. Optional ventilation tubes direct cooling and drying air through openings in the ear cups to the user's ears. Gates or shutters supported by the helmet shell allow the user to open and dose the ventilation tubes as desired.

Abstract translation: 骑自行车，摩托车或其他车辆的骑手使用的头盔。 左右听力组件安装在头盔内。 每个组件具有封闭的耳罩，其定位成紧密地贴合在用户耳朵的相应的一个上。 相应的听力管道从耳罩延伸穿过头盔的内部到相应的开口。 因此，当耳罩阻挡大量的风噪声时，听力管道将令人期望的声音（例如紧急警报器，其他交通噪音和声音）直接传送到用户的耳朵。 可以提供根据需要关闭和打开听力管道的用户可操作的致动器。 可选择的通风管通过耳罩中的开口直接冷却和干燥空气到用户的耳朵。 由头盔壳支撑的门或百叶窗允许使用者根据需要打开和剂量通气管。

公开(公告)号：USD333185S

公开(公告)日：1993-02-09

申请号：US566994

申请日：1990-08-13

Applicant: Mark Andersen ,  John von Buelow ,  Denise C. Garrison ,  Vance C. Mitchell ,  Nelson F. Thune ,  Leonard Porche

Designer： Mark Andersen ,  John von Buelow ,  Denise C. Garrison ,  Vance C. Mitchell ,  Nelson F. Thune ,  Leonard Porche