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    PROXIMITY PROBING OF TARGET PROTEINS COMPRISING RESTRICTION AND/OR EXTENSION
    2.
    发明申请
    PROXIMITY PROBING OF TARGET PROTEINS COMPRISING RESTRICTION AND/OR EXTENSION 审中-公开
    包含限制和/或扩展的目标蛋白质的临近检测

    公开(公告)号：US20070026430A1

    公开(公告)日：2007-02-01

    申请号：US11428191

    申请日：2006-06-30

    申请人： Mark Andersen ,  Benjamin Schroeder ,  Kai Lao

    发明人： Mark Andersen ,  Benjamin Schroeder ,  Kai Lao

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6813 ,  C12Q1/686 ,  C12Q2525/301 ,  C12Q2533/101 ,  C12Q2521/301

    摘要： The present teachings provide methods, compositions, and kits for detecting target analytes, including proteins. In some embodiments, cleavage reactions are performed in the context of proximity probe reactions that query target proteins, wherein the presence and/or quantity of cleavage products is indicative of the presence and/or quantity of a target protein. In some embodiments, the cleavage fragments are quantitated using a real time PCR assay comprising a stem-loop primer, wherein the stem-loop primer comprises a self-complementary hairpin structure and a free 3′ end end complementary to the cleavage product. In some embodiments, polymerase extension approaches are employed in the context of proximity probe reactions.

    摘要翻译： 本教导提供了用于检测目标分析物（包括蛋白质）的方法，组合物和试剂盒。 在一些实施方案中，裂解反应在查询靶蛋白的邻近探针反应的上下文中进行，其中切割产物的存在和/或数量指示靶蛋白的存在和/或数量。 在一些实施方案中，使用包含茎环引物的实时PCR测定法定量切割片段，其中茎环引物包含自身互补的发夹结构和与切割产物互补的游离的3'末端。 在一些实施方案中，在邻近探针反应的上下文中使用聚合酶延伸方法。

    METHYLATION ANALYSIS OF MATE PAIRS
    5.
    发明申请
    METHYLATION ANALYSIS OF MATE PAIRS 审中-公开
    MATE PAIRS的甲基化分析

    公开(公告)号：US20100120034A1

    公开(公告)日：2010-05-13

    申请号：US12498285

    申请日：2009-07-06

    申请人： Kevin McKernan ,  Benjamin Schroeder ,  Victoria Boyd

    发明人： Kevin McKernan ,  Benjamin Schroeder ,  Victoria Boyd

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6827 ,  C12Q1/686 ,  C12Q2525/307 ,  C12Q2525/191 ,  C12Q2521/331

    摘要： Various embodiments of the present teachings relate to methods for the methylation analysis of nucleic acids. The subject methods include methods that result in the preparation of mate-pair libraries suitable for highly multiplexed DNA sequencing. Embodiments include methods of preparing mate-pair libraries comprising a first tag sequence and a second tag sequence, wherein one of the tag sequences has been converted by a methylation conversion agent and the other tag sequence has not been converted by the methylation conversion agent. Other embodiments provided include intermediates for making the mate-pair library and kits for making the mate-pair libraries. Also provided is software and computer systems for analyzing the methylation levels of genomic DNA from which the tag sequences were derived.

    摘要翻译： 本发明的各种实施方案涉及核酸甲基化分析的方法。 主题方法包括导致适合于高度多重化DNA测序的配偶对文库的制备的方法。 实施方案包括制备包含第一标签序列和第二标签序列的伴侣对文库的方法，其中标签序列之一已被甲基化转化剂转化，另一个标签序列尚未被甲基化转化剂转化。 提供的其它实施方案包括用于制备配对文库和用于制备配对文库的试剂盒的中间体。 还提供了用于分析衍生标签序列的基因组DNA的甲基化水平的软件和计算机系统。

    Methods and compositions for detecting nucleic acids
    6.
    发明申请
    Methods and compositions for detecting nucleic acids 审中-公开
    用于检测核酸的方法和组合物

    公开(公告)号：US20050186606A1

    公开(公告)日：2005-08-25

    申请号：US11055849

    申请日：2005-02-11

    申请人： Benjamin Schroeder

    发明人： Benjamin Schroeder

    IPC分类号： C12N9/22 ,  C12Q1/68

    CPC分类号： C12Q1/6813

    摘要： A reporter molecule for detecting a nucleic acid is disclosed. The molecule comprises an enzyme having a kcat of at least about 200 sec−1, a reversible inhibitor of the enzyme inhibitorily engaging the enzyme, and a nucleobase polymer extending between the enzyme and the inhibitor. The polymer interferes with the engagement between the inhibitor and the enzyme when the nucleic acid contacts the polymer. Methods of making and methods of using the reporter molecule are also disclosed.

    摘要翻译： 公开了用于检测核酸的报告分子。 该分子包含具有至少约200秒-1的氨基酸的酶，与酶紧密接触的酶的可逆抑制剂和在该酶之间延伸的核碱基聚合物 和抑制剂。 当核酸与聚合物接触时，聚合物干扰抑制剂和酶之间的接合。 还公开了制备方法和使用报道分子的方法。