公开(公告)号：US20100184186A1

公开(公告)日：2010-07-22

申请号：US12691904

申请日：2010-01-22

Applicant: Po-Ting Chen ,  Chii-Gong Tong ,  Tuan-Hua David Ho ,  Su-May Yu

Inventor： Po-Ting Chen ,  Chii-Gong Tong ,  Tuan-Hua David Ho ,  Su-May Yu

IPC: C12N9/02 ,  C07H21/04 ,  C12N15/63 ,  C12N1/15

CPC classification number: C12N9/0061

Abstract: An isolated R. microporus laccase, a nucleic acid encoding the laccase, and a method of preparing it in a cell.

Abstract translation: 分离的微孔漆酶漆酶，编码漆酶的核酸，以及在细胞中制备它的方法。

公开(公告)号：US20090313725A1

公开(公告)日：2009-12-17

申请号：US12139674

申请日：2008-06-16

Applicant: Su-May YU ,  Shuen-Fang LO ,  Liang-Jwu CHEN

Inventor： Su-May YU ,  Shuen-Fang LO ,  Liang-Jwu CHEN

IPC: C12N15/82 ,  C12N15/29 ,  C12N15/52 ,  C07K14/415 ,  C12Q1/68 ,  C12N5/10 ,  C12N1/21 ,  A01H5/00

CPC classification number: C12N15/8297 ,  C12N9/0071

Abstract: Novel gibberellin 2-oxidase (GA2ox) genes were identified. Differential expression of GA2ox genes correlated with flower development, seed germination, tiller growth and other developmental processes. In addition, the early and increased growth of tiller and adventitious root and altered root architecture caused by overexpression of GA2oxs further suggest the pleiotropic role of GA2oxs in controlling growth and architecture in plants such as rice. GA2ox5, GA2ox6 and GA2ox9 were three genes encoding class C20 GA2oxs in rice. Mutants or transgenic rice overexpressing class C20 GA2oxs exhibited a broad range of mutant phenotypes, including semi-dwarfism, increased root system and higher tiller numbers that may favor grain yield. Mutations in the conserved domain III were found to affect the physiological activity of class C20 GA2oxs.

Abstract translation: 鉴定了新型赤霉素2-氧化酶（GA2ox）基因。 GA2ox基因的差异表达与花的发育，种子发芽，分蘖生长等发育过程相关。 此外，由于GA2ox过表达引起的分蘖和不定根的早期增长和根系的改变进一步表明GA2oxs在控制水稻等植物生长和结构方面具有多方位的作用。 GA2ox5，GA2ox6和GA2ox9是水稻中编码C20 GA2oxs的三个基因。 突变体或转基因水稻超过C20类型的GA2oxs表现出广泛的突变表型，包括半侏儒症，增加的根系和较高的分蘖数，可能有利于谷物产量。 发现保守结构域III中的突变会影响C20类GA2氧化酶的生理活性。

公开(公告)号：US07411115B2

公开(公告)日：2008-08-12

申请号：US10989719

申请日：2004-11-15

Applicant: Su-May Yu

Inventor： Su-May Yu

IPC: A01H5/00 ,  C12N15/52 ,  C12N15/82

CPC classification number: C12N15/8257 ,  C12N15/8223 ,  C12N15/8225 ,  C12N15/8226

Abstract: A transformed tuberous plant cell or a transgenic tuberous plant containing a heterologous nucleic acid that includes a sweet potato sporamin promoter operably linked to a sequence encoding a polypeptide.

Abstract translation: 转化的块茎植物细胞或含有异源核酸的转基因块茎植物，其包含与编码多肽的序列可操作地连接的甘薯孢子淀粉启动子。

公开(公告)号：US20050066387A1

公开(公告)日：2005-03-24

申请号：US10819371

申请日：2004-04-05

Applicant: Su-May Yu ,  Chwan-Yang Hong

Inventor： Su-May Yu ,  Chwan-Yang Hong

IPC: C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  C07H21/04 ,  C12N5/04 ,  C12N9/48

CPC classification number: C12N15/8234 ,  C12N15/8257

Abstract: A nucleic acid containing a glutelin gene promoter. Disclosed are transformed plant cells and transgenic plants containing a nucleic acid that includes the promoter operably linked to a sequence encoding heterologous protein. Also disclosed are methods of making the transformed plant cells and transgenic plants and methods for expressing a polypeptide.

Abstract translation: 含有谷蛋白基因启动子的核酸。 公开了转化的植物细胞和含有核酸的转基因植物，所述核酸包括可操作地连接到编码异源蛋白质的序列的启动子。 还公开了制备转化的植物细胞和转基因植物的方法以及用于表达多肽的方法。

公开(公告)号：US5969127A

公开(公告)日：1999-10-19

申请号：US951718

申请日：1997-10-16

Applicant: Su-May Yu ,  Ming-Tsair Chan

Inventor： Su-May Yu ,  Ming-Tsair Chan

IPC: C12N9/32 ,  C12N15/29 ,  C12N15/56 ,  C12N15/84 ,  A01H4/00 ,  C12N15/82

CPC classification number: C12N9/2422

Abstract: An expression vector comprising a promoter capable of directing expression of a coding sequence in an angiosperm cell, and a 3' untranslated region of a rice .alpha.-amylase gene, wherein, after the coding sequence is inserted into the vector, the 3' untranslated region and the coding sequence are transcribed into a single mRNA. Also featured are methods of producing a polypeptide in angiosperm cells using such a vector.

Abstract translation: 包含能够引导被子植物细胞中的编码序列表达的启动子和水稻α-淀粉酶基因的3'非翻译区的表达载体，其中在编码序列插入载体后，3'非翻译区 编码序列被转录成单个mRNA。 还特征在于使用这种载体在被子植物细胞中产生多肽的方法。

公开(公告)号：US5917029A

公开(公告)日：1999-06-29

申请号：US38227

申请日：1998-03-11

Applicant: Su-May Yu

Inventor： Su-May Yu

IPC: C12N15/55 ,  C12N15/82 ,  C12N15/11

CPC classification number: C12N15/8238

Abstract: Sugar-responsive enhancers derived from cereal .alpha.-amylase genes. Also featured are expression vectors and other isolated DNA's containing such enhancers, as well as uses of the expression vectors.

Abstract translation: 衍生自谷物α-淀粉酶基因的糖反应性增强子。 还具有表达载体和含有这些增强子的其他分离的DNA，以及表达载体的用途。

公开(公告)号：US5460952A

公开(公告)日：1995-10-24

申请号：US973324

申请日：1992-11-04

Applicant: Su-May Yu ,  Li-Fei Liu

Inventor： Su-May Yu ,  Li-Fei Liu

IPC: C12N15/09 ,  A01H5/00 ,  C12N5/00 ,  C12N5/10 ,  C12N15/82 ,  C12P21/02 ,  C12R1/91 ,  C12P21/06 ,  C12N15/00

CPC classification number: C12N15/8238 ,  C12N15/8222 ,  C12N15/8234

Abstract: Disclosed is a method for producing a gene product by expressing a gene encoding said gene product in plant host cells, comprising the steps of: constructing a vector expressible in plant host cells, the vector comprising a promoter region derived from an .alpha.-amylase gene of a plant, and a gene encoding a desired gene product; transforming a compatible plant host cell with the vector; cultivating the resultant transformant host cell; subjecting the cultivated transformant host cell to a sugar-depleted or sugar-free condition to promote the expression of the gene under the control of the promoter region; and recovering the expressed gene product. Also disclosed is a method for producing a gene product method which comprises the steps of: constructing a vector expressible in plant host cells, the vector comprising a promoter region derived from an .alpha.-amylase gene of a plant, and a gene encoding a desired gene product, the promoter region including the promoter and a DNA sequence encoding the signal peptide; transforming a compatible plant host cell with the vector; cultivating the resultant transformant host cell in a suitable culture medium; and directly recovering the expressed gene product from the medium.

Abstract translation: 公开了通过在植物宿主细胞中表达编码所述基因产物的基因来产生基因产物的方法，包括以下步骤：构建在植物宿主细胞中可表达的载体，所述载体包含衍生自α-淀粉酶基因的启动子区 植物和编码所需基因产物的基因; 用载体转化相容的植物宿主细胞; 培养所得转化体宿主细胞; 对培养的转化体宿主细胞进行糖消化或无糖条件以促进基因在启动子区域控制下的表达; 并回收表达的基因产物。 还公开了一种生产基因产物方法的方法，其包括以下步骤：构建在植物宿主细胞中可表达的载体，所述载体包含源自植物的α-淀粉酶基因的启动子区域和编码所需基因的基因 产物，包括启动子的启动子区域和编码信号肽的DNA序列; 用载体转化相容的植物宿主细胞; 在合适的培养基中培养所得的转化体宿主细胞; 并从培养基中直接回收表达的基因产物。