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    Microrna as ligands and target molecules
    32.
    发明申请
    Microrna as ligands and target molecules 审中-公开
    Microrna作为配体和靶分子

    公开(公告)号：US20050142581A1

    公开(公告)日：2005-06-30

    申请号：US10934798

    申请日：2004-09-03

    申请人： Richard Griffey ,  C. Bennett ,  David Ecker ,  Donna Ward ,  Susan Freier

    发明人： Richard Griffey ,  C. Bennett ,  David Ecker ,  Donna Ward ,  Susan Freier

    IPC分类号： C12N20060101 ,  C12N15/11 ,  C12N15/113 ,  C12Q1/68

    CPC分类号： C12N15/111 ,  C12N15/1138 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2320/11 ,  C12N2330/10 ,  C12N2310/3525

    摘要： The present invention provides methods for the identification of target molecules that bind to ligands, particularly microRNA ligands and mimics thereof and/or microRNA target molecules and mimics thereof, with as little as millimolar (mM) affinity using mass spectrometry. The methods may be used to determine the mode of binding interaction between two or more of these target molecules to the ligand as well as their relative affinities. Also provided are methods for designing compounds having greater affinity to a ligand by identifying two or more target molecules using mass spectrometry methods of the invention and linking the target molecules together to form a novel compound.

    摘要翻译： 本发明提供用于使用质谱法以少至毫摩尔（mM）亲和力鉴定结合配体，特别是微小RNA配体及其模拟物和/或微小RNA靶分子及其模拟物的靶分子的方法。 该方法可用于确定这些靶分子中两个或更多个与配体之间的结合相互作用的模式以及它们的相对亲和力。 还提供了通过使用本发明的质谱方法鉴定两个或更多个靶分子并将靶分子连接在一起以形成新化合物来设计对配体具有更大亲和力的化合物的方法。