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公开(公告)号：US08389257B2
公开(公告)日：2013-03-05
申请号：US13328327
申请日：2011-12-16
CPC分类号： C12N9/18 , C11D3/38636 , C11D3/3945 , C12P7/40 , C12Y301/01072 , D21C9/166
摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
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公开(公告)号：US08389256B2
公开(公告)日：2013-03-05
申请号：US13328305
申请日：2011-12-16
CPC分类号： C11D3/3945 , C12N9/18 , C12P7/40 , C12P7/54 , C12Y301/01072 , D21C5/005 , D21C9/166
摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
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公开(公告)号：US08222012B2
公开(公告)日：2012-07-17
申请号：US12571702
申请日：2009-10-01
CPC分类号： C11D3/386 , C11D3/2093 , C11D3/38636 , C11D3/39 , C11D7/266 , C12N9/18 , C12P7/40
摘要： A process is provided for rapidly producing target concentrations of peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with a source of peroxygen, such as hydrogen peroxide, in the presence of an enzyme catalyst comprising an enzyme having identity to an acetyl xylan esterase from Lactococcus lactis having perhydrolysis activity. The polypeptide is an enzyme structurally classified as a member of the carbohydrate esterase family 7 (CE-7). The peroxycarboxylic acids produced by the present process can be used in disinfecting, bleaching, and other laundry care applications. Compositions comprising the reaction components and the peroxycarboxylic acids produced by the process are also provided.
摘要翻译： 提供了一种从羧酸酯快速产生过氧羧酸的目标浓度的方法。 更具体地，羧酸酯与过氧化物源如过氧化氢在含有与具有过水解活性的乳酸乳球菌的乙酰木聚糖酯酶同一性的酶催化剂存在下反应。 多肽是结构上分类为碳水化合物酯酶家族7（CE-7）的成员的酶。 通过本方法生产的过氧羧酸可用于消毒，漂白和其他洗衣护理应用。 还提供了包含由该方法生产的反应组分和过氧羧酸的组合物。
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公开(公告)号：US08206964B2
公开(公告)日：2012-06-26
申请号：US13070149
申请日：2011-03-23
CPC分类号： C12N9/18 , C11D3/38636 , C11D3/3945 , C12P7/40
摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
摘要翻译： 提供具有过水解活性的乙酰木聚糖酯酶变体，用于从羧酸酯和过氧源产生过氧羧酸。 更具体地，使用易错PCR和定点诱变来修饰热转录海马乙酰木聚糖酯酶基因，以产生特征在于比活性增加的酶催化剂。 变体乙酰木聚糖酯酶可用于制备适用于各种用途的过氧羧酸，例如清洁，消毒，消毒，漂白，木浆加工和纸浆加工应用。
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公开(公告)号：US20120156156A1
公开(公告)日：2012-06-21
申请号：US13328327
申请日：2011-12-16
IPC分类号： C12P7/40 , C12N15/63 , C12N1/15 , C12N1/19 , C12N1/21 , D21C9/16 , A01N37/16 , A01P1/00 , A61L2/16 , C12M1/40 , D06L3/02 , C12N15/55 , C12N9/18
CPC分类号： C12N9/18 , C11D3/38636 , C11D3/3945 , C12P7/40 , C12Y301/01072 , D21C9/166
摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
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公开(公告)号：US08178581B2
公开(公告)日：2012-05-15
申请号：US12722298
申请日：2010-03-11
CPC分类号： A01N37/02 , A01N37/16 , A01N37/36 , A01N59/00 , A61L2/186 , C12P7/40 , C12P7/62 , A01N63/02 , A01N2300/00
摘要： A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided.
摘要翻译： 提供了从羧酸酯制备过氧羧酸的方法。 更具体地说，羧酸酯与无机过氧化物如过氧化氢在具有过水解活性的酶催化剂存在下反应。 基于保守的结构特征，本发明的过水解酶催化剂被分类为碳水化合物酯酶家族7（CE-7）的成员。 此外，提供包含通过本文所述方法生产的过酸的消毒剂制剂。
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公开(公告)号：US08114908B2
公开(公告)日：2012-02-14
申请号：US12497348
申请日：2009-07-02
CPC分类号： C11D3/38636 , A01N37/16 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/3947 , C11D3/48 , C12P7/40 , A01N37/02 , A01N37/12 , A01N37/46 , A01N43/16 , A01N59/00 , A01N61/00 , A01N2300/00
摘要： A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided.
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公开(公告)号：US20110236338A1
公开(公告)日：2011-09-29
申请号：US13070144
申请日：2011-03-23
IPC分类号： A61L2/16 , C12N15/63 , C12N1/00 , C12N9/18 , C12P7/24 , C07H21/04 , A01N37/16 , D21C9/16 , D06L3/02 , A01P1/00
CPC分类号： C12N9/18
摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
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公开(公告)号：US20110236337A1
公开(公告)日：2011-09-29
申请号：US13070135
申请日：2011-03-23
IPC分类号： C12N9/18 , C12N15/63 , C12N1/00 , C12P7/24 , C07H21/04 , A61L2/16 , A61L9/00 , D06L3/02 , D21C9/10
CPC分类号： C12N9/18 , C11D3/38636 , C12P7/40 , C12P7/54
摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
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公开(公告)号：US07951567B2
公开(公告)日：2011-05-31
申请号：US12691856
申请日：2010-01-22
摘要： A method is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity derived from Bacillus sp. to produce peroxycarboxylic acids.
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