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    TRANSGENIC PLANTS WITH IMPROVED SACCHARIFICATION YIELDS AND METHODS OF GENERATING SAME
    1.
    发明申请
    TRANSGENIC PLANTS WITH IMPROVED SACCHARIFICATION YIELDS AND METHODS OF GENERATING SAME 审中-公开
    具有改进的收敛性的转基因植物及其产生方法

    公开(公告)号：US20130227724A1

    公开(公告)日：2013-08-29

    申请号：US13883046

    申请日：2011-11-03

    申请人： Miron Abramson ,  Ziv Shani ,  Oded Shoseyov

    发明人： Miron Abramson ,  Ziv Shani ,  Oded Shoseyov

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8255 ,  C12N9/18 ,  C12N15/8243 ,  C12N15/8246 ,  C12Y301/01072

    摘要： A method of engineering a plant having reduced acetylation in a cell wall is disclosed. The method comprising expressing in the plant cell wall at least one isolated heterologous polynucleotide encoding an acetylxylan esterase (AXE) enzyme under the transcriptional control of a developmentally regulated promoter specifically active in the plant cell wall upon secondary cell wall deposit, thereby engineering the plant having reduced acetylation in the cell wall.

    摘要翻译： 公开了一种在细胞壁中工程化乙酰化减少的植物的方法。 所述方法包括在植物细胞壁上表达至少一种编码乙酰木聚糖酯酶（AX）酶的分离的异源多核苷酸，其在二次细胞壁沉积物上在植物细胞壁中特异性活化的发育调节启动子的转录控制下，由此工程化具有 减少细胞壁中的乙酰化。

    Perhydrolase providing improved peracid stability
    3.
    发明授权
    Perhydrolase providing improved peracid stability 有权

    公开(公告)号：US07960151B1

    公开(公告)日：2011-06-14

    申请号：US13036752

    申请日：2011-02-28

    申请人： Robert DiCosimo ,  John Edward Gavagan ,  Mark S. Payne

    发明人： Robert DiCosimo ,  John Edward Gavagan ,  Mark S. Payne

    IPC分类号： C12P7/62 ,  C12N9/18 ,  C11D3/00

    CPC分类号： C12N9/18 ,  A61L2/186 ,  C12P7/40 ,  C12Y301/01072

    摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

    Perhydrolase variant providing improved specific activity
    8.
    发明授权
    Perhydrolase variant providing improved specific activity 失效
    过水解酶变体提供改善的比活性

    公开(公告)号：US08389260B2

    公开(公告)日：2013-03-05

    申请号：US13328418

    申请日：2011-12-16

    申请人： Robert DiCosimo ,  Mark Scott Payne ,  John Edward Gavagan

    发明人： Robert DiCosimo ,  Mark Scott Payne ,  John Edward Gavagan

    IPC分类号： C12N9/18 ,  C12P7/40 ,  A61L9/00

    CPC分类号： C12N9/18 ,  C12Y301/01072

    摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritime acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

    摘要翻译： 提供具有过水解活性的乙酰木聚糖酯酶变体，用于从羧酸酯和过氧源产生过氧羧酸。 更具体地，使用易错PCR和定点诱变来修饰Thermotoga海洋乙酰木聚糖酯酶基因，以产生特征在于比活性增加的酶催化剂。 变体乙酰木聚糖酯酶可用于制备适用于各种用途的过氧羧酸，例如清洁，消毒，消毒，漂白，木浆加工和纸浆加工应用。

    PERHYDROLASE VARIANT PROVIDING IMPROVED SPECIFIC ACTIVITY
    10.
    发明申请
    PERHYDROLASE VARIANT PROVIDING IMPROVED SPECIFIC ACTIVITY 失效
    PERHYDROLASE变体提供改进的特定活性

    公开(公告)号：US20120156162A1

    公开(公告)日：2012-06-21

    申请号：US13328418

    申请日：2011-12-16

    申请人： Robert Dicosimo ,  Mark Scott Payne ,  John Edward Gavagan

    发明人： Robert Dicosimo ,  Mark Scott Payne ,  John Edward Gavagan

    IPC分类号： A61L9/01 ,  C12N9/16 ,  C12N1/21 ,  D21H21/32 ,  A01N37/02 ,  C07H21/04 ,  D06L3/00 ,  C12P7/62 ,  C12N15/63

    CPC分类号： C12N9/18 ,  C12Y301/01072

    摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritime acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

    摘要翻译： 提供具有过水解活性的乙酰木聚糖酯酶变体，用于从羧酸酯和过氧源产生过氧羧酸。 更具体地，使用易错PCR和定点诱变来修饰Thermotoga海洋乙酰木聚糖酯酶基因，以产生特征在于比活性增加的酶催化剂。 变体乙酰木聚糖酯酶可用于制备适用于各种用途的过氧羧酸，例如清洁，消毒，消毒，漂白，木浆加工和纸浆加工应用。