公开(公告)号：US08445242B2

公开(公告)日：2013-05-21

申请号：US13070130

申请日：2011-03-23

Applicant: Robert DiCosimo ,  John Edward Gavagan ,  Mark Scott Payne

Inventor： Robert DiCosimo ,  John Edward Gavagan ,  Mark Scott Payne

IPC: C12P7/24 ,  C12N9/00 ,  C12N9/16 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC classification number: C11D3/38636 ,  C11D3/3945 ,  C12N9/18 ,  C12P7/40 ,  C12Y301/01072 ,  D21C9/166

Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

公开(公告)号：US08394616B2

公开(公告)日：2013-03-12

申请号：US13328350

申请日：2011-12-16

Applicant: Robert DiCosimo ,  Mark Scott Payne ,  John Edward Gavagan

Inventor： Robert DiCosimo ,  Mark Scott Payne ,  John Edward Gavagan

IPC: C12N9/18 ,  C12P7/40 ,  A61L9/00

CPC classification number: C11D3/38636 ,  C11D3/2093 ,  C11D3/3942 ,  C11D3/3947 ,  C12N9/18 ,  C12P7/40 ,  C12Y301/01072 ,  D21C9/166

Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

公开(公告)号：US08389259B2

公开(公告)日：2013-03-05

申请号：US13328381

申请日：2011-12-16

Applicant: Robert DiCosimo ,  Mark Scott Payne ,  John Edward Gavagan

Inventor： Robert DiCosimo ,  Mark Scott Payne ,  John Edward Gavagan

IPC: C12N9/18 ,  C12P7/40 ,  A61L9/00

CPC classification number: A61L2/186 ,  A61L2/22 ,  A61L2/23 ,  A61L2202/24 ,  C12N9/18 ,  C12Y301/01072

Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

公开(公告)号：US08389257B2

公开(公告)日：2013-03-05

申请号：US13328327

申请日：2011-12-16

Applicant: Robert Dicosimo ,  Mark Scott Payne ,  John Edward Gavagan

Inventor： Robert Dicosimo ,  Mark Scott Payne ,  John Edward Gavagan

IPC: C12N9/18 ,  C12P7/40 ,  A61L9/00

CPC classification number: C12N9/18 ,  C11D3/38636 ,  C11D3/3945 ,  C12P7/40 ,  C12Y301/01072 ,  D21C9/166

Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

公开(公告)号：US08389256B2

公开(公告)日：2013-03-05

申请号：US13328305

申请日：2011-12-16

Applicant: Robert Dicosimo ,  Mark Scott Payne ,  John Edward Gavagan

Inventor： Robert Dicosimo ,  Mark Scott Payne ,  John Edward Gavagan

IPC: C12N9/18 ,  C12P7/40 ,  A61L9/00

CPC classification number: C11D3/3945 ,  C12N9/18 ,  C12P7/40 ,  C12P7/54 ,  C12Y301/01072 ,  D21C5/005 ,  D21C9/166

Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

公开(公告)号：US08222012B2

公开(公告)日：2012-07-17

申请号：US12571702

申请日：2009-10-01

Applicant: Robert DiCosimo ,  John Edward Gavagan ,  Mark Scott Payne

Inventor： Robert DiCosimo ,  John Edward Gavagan ,  Mark Scott Payne

IPC: C12P7/14 ,  C12N9/14 ,  C12N15/00 ,  C12N1/21

CPC classification number: C11D3/386 ,  C11D3/2093 ,  C11D3/38636 ,  C11D3/39 ,  C11D7/266 ,  C12N9/18 ,  C12P7/40

Abstract: A process is provided for rapidly producing target concentrations of peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with a source of peroxygen, such as hydrogen peroxide, in the presence of an enzyme catalyst comprising an enzyme having identity to an acetyl xylan esterase from Lactococcus lactis having perhydrolysis activity. The polypeptide is an enzyme structurally classified as a member of the carbohydrate esterase family 7 (CE-7). The peroxycarboxylic acids produced by the present process can be used in disinfecting, bleaching, and other laundry care applications. Compositions comprising the reaction components and the peroxycarboxylic acids produced by the process are also provided.

Abstract translation: 提供了一种从羧酸酯快速产生过氧羧酸的目标浓度的方法。 更具体地，羧酸酯与过氧化物源如过氧化氢在含有与具有过水解活性的乳酸乳球菌的乙酰木聚糖酯酶同一性的酶催化剂存在下反应。 多肽是结构上分类为碳水化合物酯酶家族7（CE-7）的成员的酶。 通过本方法生产的过氧羧酸可用于消毒，漂白和其他洗衣护理应用。 还提供了包含由该方法生产的反应组分和过氧羧酸的组合物。

公开(公告)号：US08206964B2

公开(公告)日：2012-06-26

申请号：US13070149

申请日：2011-03-23

Applicant: Robert DiCosimo ,  John Edward Gavagan ,  Mark Scott Payne

Inventor： Robert DiCosimo ,  John Edward Gavagan ,  Mark Scott Payne

IPC: C12N9/18 ,  C12P7/40 ,  A01N37/00

CPC classification number: C12N9/18 ,  C11D3/38636 ,  C11D3/3945 ,  C12P7/40

Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

Abstract translation: 提供具有过水解活性的乙酰木聚糖酯酶变体，用于从羧酸酯和过氧源产生过氧羧酸。 更具体地，使用易错PCR和定点诱变来修饰热转录海马乙酰木聚糖酯酶基因，以产生特征在于比活性增加的酶催化剂。 变体乙酰木聚糖酯酶可用于制备适用于各种用途的过氧羧酸，例如清洁，消毒，消毒，漂白，木浆加工和纸浆加工应用。

公开(公告)号：US20120156156A1

公开(公告)日：2012-06-21

申请号：US13328327

申请日：2011-12-16

Applicant: Robert Dicosimo ,  Mark Scott Payne ,  John Edward Gavagan

Inventor： Robert Dicosimo ,  Mark Scott Payne ,  John Edward Gavagan

IPC: C12P7/40 ,  C12N15/63 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  D21C9/16 ,  A01N37/16 ,  A01P1/00 ,  A61L2/16 ,  C12M1/40 ,  D06L3/02 ,  C12N15/55 ,  C12N9/18

CPC classification number: C12N9/18 ,  C11D3/38636 ,  C11D3/3945 ,  C12P7/40 ,  C12Y301/01072 ,  D21C9/166

Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

公开(公告)号：US08178581B2

公开(公告)日：2012-05-15

申请号：US12722298

申请日：2010-03-11

Applicant: Robert Dicosimo ,  John Edward Gavagan ,  Mark Scott Payne ,  Frederick B. Cooling, III

Inventor： Robert Dicosimo ,  John Edward Gavagan ,  Mark Scott Payne ,  Frederick B. Cooling, III

IPC: A01N37/00 ,  C12P7/40 ,  C12N9/14

CPC classification number: A01N37/02 ,  A01N37/16 ,  A01N37/36 ,  A01N59/00 ,  A61L2/186 ,  C12P7/40 ,  C12P7/62 ,  A01N63/02 ,  A01N2300/00

Abstract: A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided.

Abstract translation: 提供了从羧酸酯制备过氧羧酸的方法。 更具体地说，羧酸酯与无机过氧化物如过氧化氢在具有过水解活性的酶催化剂存在下反应。 基于保守的结构特征，本发明的过水解酶催化剂被分类为碳水化合物酯酶家族7（CE-7）的成员。 此外，提供包含通过本文所述方法生产的过酸的消毒剂制剂。

公开(公告)号：US08129153B2

公开(公告)日：2012-03-06

申请号：US12539025

申请日：2009-08-11

Applicant: Robert DiCosimo ,  Mark Scott Payne ,  Eugenia Costa Hann

Inventor： Robert DiCosimo ,  Mark Scott Payne ,  Eugenia Costa Hann

IPC: C12P7/40 ,  C12N9/14 ,  C12N15/00 ,  C12N1/21

CPC classification number: C12N9/18 ,  C11D3/0047 ,  C11D3/38636 ,  C11D3/38681 ,  C11D3/3945 ,  C11D3/48

Abstract: A process is provided for producing target concentrations of peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity under conditions where control of reaction pH by selection of buffer concentration and concentration of perhydrolase and reactants produces a targeted concentration of peroxycarboxylic acids. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided, as are corresponding methods of use.

Abstract translation: 提供了从羧酸酯生产过氧羧酸的目标浓度的方法。 更具体地说，羧酸酯与无机过氧化物如过氧化氢反应，在具有过水解活性的酶催化剂的存在下，通过选择缓冲液浓度和过水解酶和反应物的浓度来控制反应pH产生目标浓度 的过氧羧酸。 基于保守的结构特征，本发明的过水解酶催化剂被分类为碳水化合物酯酶家族7（CE-7）的成员。 此外，提供包含通过本文所述方法生产的过酸的消毒剂制剂，以及相应的使用方法。