公开(公告)号：US20200009556A1

公开(公告)日：2020-01-09

申请号：US16457667

申请日：2019-06-28

Applicant: ILLUMINA, Inc.

Inventor： Maxwell Zimmerley ,  LiangLiang Qiang ,  M. Shane Bowen ,  Steven H. Modiano ,  Dajun Yuan ,  Randall Smith ,  Arthur J. Pitera ,  Hai Quang Tran ,  Gerald Kreindl

IPC: B01L3/00

Abstract: An interposer for a flow cell comprises a base layer having a first surface and a second surface opposite the first surface. The base layer comprises black polyethylene terephthalate (PET). A first adhesive layer is disposed on the first surface of the base layer. The first adhesive layer comprises methyl acrylic adhesive. A second adhesive layer is disposed on the second surface of the base layer. The second adhesive layer comprises methyl acrylic adhesive. A plurality of microfluidic channels extends through each of the base layer, the first adhesive layer, and the second adhesive layer.

公开(公告)号：US10287629B2

公开(公告)日：2019-05-14

申请号：US15207892

申请日：2016-07-12

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  Xiaohai Liu ,  Wenyi Feng ,  Bernard Hirschbein ,  Helmy A. Eltoukhy ,  Xiaolin Wu ,  Geoffrey Paul Smith ,  Jonathan Mark Boutell ,  Thomas Joseph ,  Randall Smith ,  Min-Jui Richard Shen ,  Carolyn Tregidgo ,  Kay Klausing

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6869

Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

公开(公告)号：US20180195115A1

公开(公告)日：2018-07-12

申请号：US15848717

申请日：2017-12-20

Applicant: ILLUMINA, INC.

Inventor： Peyton Shieh ,  John M. Beierle ,  Michael S. Graige ,  Alexander Fuhrmann ,  Randall Smith ,  Wei Wei ,  Naiqian Zhan

IPC: C12Q1/6837

CPC classification number: C12Q1/6837 ,  C12Q2565/507

Abstract: An array includes a support including a plurality of discrete wells, a gel material positioned in each of the plurality of discrete wells, and a quality control tracer grafted to the gel material in each of the plurality of discrete wells. The quality control tracer comprises (a) a cleavable nucleotide sequence comprising a cleavage site and (b) a detectable label; and in some aspects, is a cleavable nucleotide sequence with a detectable label and a non-reactive nucleotide sequence or a primer nucleotide sequence.

公开(公告)号：US09475831B2

公开(公告)日：2016-10-25

申请号：US15002258

申请日：2016-01-20

Applicant: Illumina, Inc.

Inventor： Ryan Christopher Smith ,  Randall Smith ,  Xiaodong Zhao

IPC: C07F9/572 ,  C07F9/6512 ,  C07F9/6509 ,  A61K31/675 ,  C07F9/6503 ,  C07F9/6506 ,  C07H21/00

CPC classification number: C07F9/5728 ,  A61K31/675 ,  C07F9/65031 ,  C07F9/6506 ,  C07H21/00

Abstract: A compound has Formula I: A, B, C, D, W, X, Y, and Z are independently selected from hydrogen, optionally substituted C1-C6 alkyl, optionally substituted C1-C6 alkoxy, aryl, aldehyde, protected aldehyde, CH, N, O, S, null, and bond; Q is selected from aldehyde, protected aldehyde, and null, at least one of A, B, C, D, W, X, Y, Z, or Q is an aldehyde or protected aldehyde; the bonds between each of A-B, B-C, C-D, W-X, X-Y, and Y-Z are selected from single bond, double bond, triple bond, and no bond; L is a linker selected from a C1-C12 alkyl, aralkyl, and aryl, any of which is optionally substituted; one or more methylene unit (CH2) of the C1-C12 alkyl is optionally replaced by any combination of oxygen, carbonyl(C═O), and NH; and R1 and R2 are independently selected from —NR3R4, halogen, C1-C8 alkoxy, aralkoxy, alkenyloxy, alkynyloxy, and OCH2CH2CN; R3 and R4 are independently a C1-C4, straight chain or branched alkyl group.