公开(公告)号：US20240141428A1

公开(公告)日：2024-05-02

申请号：US18489788

申请日：2023-10-18

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  Xiaohai Liu ,  Wenyi Feng ,  Bernard Hirschbein ,  Helmy A. Eltoukhy ,  Xiaolin Wu ,  Geoffrey Paul Smith ,  Jonathan Mark Boutell ,  Thomas Joseph ,  Randall Smith ,  Min-Jui Richard Shen ,  Carolyn Tregidgo ,  Kay Klausing

IPC: C12Q1/6874 ,  C12Q1/6869

CPC classification number: C12Q1/6874 ,  C12Q1/6869

Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

公开(公告)号：US20230272457A1

公开(公告)日：2023-08-31

申请号：US18179026

申请日：2023-03-06

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Liangliang Qiang ,  Kevin L. Gunderson ,  Kay Klausing ,  Lea Pickering ,  Cyril Delattre ,  Tarun Khurana

IPC: C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6816

CPC classification number: C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6816 ,  C12Q2533/101 ,  C12Q2535/113 ,  C12Q2535/122 ,  C12Q2563/103 ,  C12Q2563/125

Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.

公开(公告)号：US20240141427A1

公开(公告)日：2024-05-02

申请号：US18373620

申请日：2023-09-27

Applicant: ILLUMINA, INC.

Inventor： Misha Golynskiy ,  Rahman Rahman Pour ,  Jiawen Li ,  Ryan Craig ,  Hamed Tabatabaei Ghomi ,  Saurabh Nirantar ,  Hsu Myat Noe ,  Lin Hui Chang ,  Yvonne Devadas ,  Jing Wen Lim ,  Kay Klausing ,  Humberto Rojo ,  Eric Murtfeldt ,  Chris Garcia

IPC: C12Q1/6874 ,  C12N9/12

CPC classification number: C12Q1/6874 ,  C12N9/1252 ,  C12Y207/07007

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low error rate, low phasing rate, or increased incorporation rate for a second generation ffN under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US20230212667A1

公开(公告)日：2023-07-06

申请号：US18089867

申请日：2022-12-28

Applicant: ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA, INC.

Inventor： Jonathan Mark Boutell ,  Eli M. Carrami ,  Pietro Gatti Lafranconi ,  Philip Balding ,  Oliver Jon Miller ,  Kay Klausing ,  Justin Robbins ,  Xiaolin Wu

IPC: C12Q1/6874

CPC classification number: C12Q1/6874 ,  C12Q1/6806

Abstract: Polynucleotide sequencing methods for sequencing one or more polynucleotide templates use primers bound to a surface as sequencing primers. The surface primers may include at least a portion of a surface oligonucleotide used during cluster formation. The sequencing methods may be used for single stranded sequencing or double stranded sequencing. Double stranded sequencing methods may employ an enzyme that has nick-translation activity. A kit includes all the reagents needed for sequencing does not include sequencing primers. The kit may be used to accomplish the sequencing methods of the present disclosure.

公开(公告)号：US20230047225A1

公开(公告)日：2023-02-16

申请号：US17829866

申请日：2022-06-01

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Misha Golynskiy ,  Mariko Matsuura ,  Saurabh Nirantar ,  Hamed Tabatabaei Ghomi ,  Seth McDonald ,  Ryan Craig ,  Sergio Peisajovich ,  Kyrie Johnson ,  Kay Klausing ,  Eric Murtfeldt ,  Alexandra Exner

IPC: C12N9/12 ,  C12Q1/6874

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low pre-phasing rates when using ambiently stored polymerases, as well as methods and kits using the same.

公开(公告)号：US10287629B2

公开(公告)日：2019-05-14

申请号：US15207892

申请日：2016-07-12

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  Xiaohai Liu ,  Wenyi Feng ,  Bernard Hirschbein ,  Helmy A. Eltoukhy ,  Xiaolin Wu ,  Geoffrey Paul Smith ,  Jonathan Mark Boutell ,  Thomas Joseph ,  Randall Smith ,  Min-Jui Richard Shen ,  Carolyn Tregidgo ,  Kay Klausing

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6869

Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

公开(公告)号：US10106851B2

公开(公告)日：2018-10-23

申请号：US15836011

申请日：2017-12-08

Applicant: ILLUMINA, INC.

Inventor： Kay Klausing ,  Min-Jui Richard Shen ,  John Moore ,  Vincent Peter Smith ,  Kevin Hall

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6874 ,  C40B20/04 ,  C12Q1/6832 ,  C12Q1/6869

Abstract: A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.

公开(公告)号：US11634697B2

公开(公告)日：2023-04-25

申请号：US17226393

申请日：2021-04-09

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited ,  Illumina Singapore Pte. Ltd.

Inventor： Kay Klausing ,  Hamed Tabatabaei Ghomi ,  Misha Golynskiy ,  Saurabh Nirantar ,  Seth McDonald ,  Sergio Peisajovich

IPC: C12N9/12

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US11629373B2

公开(公告)日：2023-04-18

申请号：US16832309

申请日：2020-03-27

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Liangliang Qiang ,  Kevin L. Gunderson ,  Kay Klausing ,  Lea Pickering ,  Cyril Delattre ,  Tarun Khurana

IPC: C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6816

Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.

公开(公告)号：US20210348141A1

公开(公告)日：2021-11-11

申请号：US17226393

申请日：2021-04-09

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited ,  Illumina Singapore Pte. Ltd.

Inventor： Kay Klausing ,  Hamed Tabatabaei Ghomi ,  Misha Golynskiy ,  Saurabh Nirantar ,  Seth McDonald ,  Sergio Peisajovich

IPC: C12N9/12

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.