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39 条记录 (耗时 0.045 秒)

    Acylated insulin
    32.
    发明授权
    Acylated insulin 失效
    酰化胰岛素

    公开(公告)号：US5750497A

    公开(公告)日：1998-05-12

    申请号：US400256

    申请日：1995-03-08

    申请人： Svend Havelund ,  John Halstr.o slashed.m ,  Ib Jonassen ,  Asser Sloth Andersen ,  Jan Markussen

    发明人： Svend Havelund ,  John Halstr.o slashed.m ,  Ib Jonassen ,  Asser Sloth Andersen ,  Jan Markussen

    IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/28 ,  A61P3/08 ,  A61P3/10 ,  C07K14/62 ,  C12N1/19 ,  C12N1/21 ,  C12P21/02 ,  C12R1/19 ,  C12R1/865

    CPC分类号： C07K14/62 ,  A61K38/00 ,  Y10S514/866

    摘要： The present invention relates to human insulin derivatives having a protracted profile of action in which the A21 and B3 amino acid residues are, independently, any amino acid residue which can be coded for by the genetic code except Lys, Arg and Cys; Phe.sup.B1 may be deleted; the B30 amino acid residue is (a) a non-codable, lipophilic amino acid having from 10 to 24 carbon atoms in which case an acyl group of a carboxylic acid with up to 5 carbon atoms is bound to the E-amino group of Lys.sup.B29 ; (b) any amino acid residue which can be coded by the genetic code except Lys, Arg and Cys, in which case a lipophilic substituent is bound to the E-amino group of Lys.sup.B29 ; or (c) deleted, in which case a lipophilic substituent is bound to the E-amino group of LyS.sup.B29 ; and any Zn.sup.2+ complexes thereof; provided that when the B30 amino acid residue is Thr or Ala, the A21 and B3 amino acid residues are both Asn and Phe.sup.B1 is present, then the insulin derivative is a Zn.sup.2 + complex.

    摘要翻译： 本发明涉及具有延长的作用曲线的人胰岛素衍生物，其中A21和B3氨基酸残基独立地是可由除Lys，Arg和Cys之外的遗传密码编码的任何氨基酸残基; PheB1可能被删除; B30氨基酸残基是（a）具有10-24个碳原子的非可编码的亲脂性氨基酸，在这种情况下，具有至多5个碳原子的羧酸的酰基与LysB29的E-氨基结合 ; （b）除了Lys，Arg和Cys之外可以通过遗传密码编码的任何氨基酸残基，在这种情况下，亲油性取代基与LysB29的E氨基结合; 或（c）缺失，在这种情况下，亲油取代基与LySB29的E氨基结合; 及其任何Zn2 +配合物; 条件是当B30氨基酸残基是Thr或Ala时，A21和B3氨基酸残基都是Asn和PheB1，则胰岛素衍生物是Zn 2+络合物。

    Acylated insulin
    36.
    发明授权
    Acylated insulin 失效
    酰化胰岛素

    公开(公告)号：US06869930B1

    公开(公告)日：2005-03-22

    申请号：US09398365

    申请日：1999-09-17

    申请人： Svend Havelund ,  John Halstrom ,  Ib Jonassen ,  Asser Sloth Andersen ,  Jan Markussen

    发明人： Svend Havelund ,  John Halstrom ,  Ib Jonassen ,  Asser Sloth Andersen ,  Jan Markussen

    IPC分类号： A61K38/00 ,  C07K14/62 ,  A61K38/28

    CPC分类号： C07K14/62 ,  A61K38/00 ,  Y10S514/866

    摘要： The present invention relates to protracted human insulin derivatives in which the A21 and the B3 amino acid residues are, independently, any amino acid residue which can be coded for by the genetic code except Lys, Arg and Cys; PheB1 may be deleted; the B30 amino acid residue is (a) a non-codable, lipophilic amino acid having from 10 to 24 carbon atoms, in which case an acyl group of a carboxylic acid with up to 5 carbon atoms is bound to the ε-amino group of LysB29; or (b) the B30 amino acid residue is deleted or is any amino acid residue which can be coded for by the genetic code except Lys, Arg and Cys, in any of which cases the ε-amino group of LysB29 has a lipophilic substituent; and any Zn2+ complexes thereof with the proviso that when B30 is Thr or Ala and A21 and B3 are both Asn, and PheB1 is present, then the insulin derivative is always present as a Zn2+ complex.

    摘要翻译： 本发明涉及延长的人胰岛素衍生物，其中A21和B3氨基酸残基独立地是可由遗传密码编码的除Lys，Arg和Cys之外的任何氨基酸残基; Phe 可能被删除; B30氨基酸残基是（a）具有10-24个碳原子的不可编码的亲脂性氨基酸，在这种情况下，具有至多5个碳原子的羧酸的酰基与ε-氨基的ε-氨基结合 Lys ; 或（b）B30氨基酸残基被缺失，或者是任何氨基酸残基，其可以通过除Lys，Arg和Cys之外的遗传密码进行编码，在这些情况下，Lys 的ε-氨基具有 亲油取代基; 和任何Zn 2+络合物，条件是当B30是Thr或Ala，A21和B3都是Asn和Phe 时，胰岛素衍生物总是作为Zn 2+络合物存在 。

    Acylated insulin
    38.
    发明授权
    Acylated insulin 失效
    酰化胰岛素

    公开(公告)号：US6011007A

    公开(公告)日：2000-01-04

    申请号：US975365

    申请日：1997-11-20

    申请人： Svend Havelund ,  John Halstrom ,  Ib Jonassen ,  Asser Sloth Andersen ,  Jan Markussen

    发明人： Svend Havelund ,  John Halstrom ,  Ib Jonassen ,  Asser Sloth Andersen ,  Jan Markussen

    IPC分类号： A61K38/00 ,  C07K14/62 ,  A61K38/28

    CPC分类号： C07K14/62 ,  A61K38/00 ,  Y10S514/866

    摘要： The present invention relates to protracted human insulin derivatives in which the A21 and the B3 amino acid residues are, independently, any amino acid residue which can be coded for by the genetic code except Lys, Arg and Cys; Phe.sup.B1 may be deleted; the B30 amino acid residue is (a) a non-codable, lipophilic amino acid having from 10 to 24 carbon atoms, in which case an acyl group of a carboxylic acid with up to 5 carbon atoms is bound to the .epsilon.-amino group of Lys.sup.B29 ; or (b) the B30 amino acid residue is deleted or is any amino acid residue which can be coded for by the genetic code except Lys, Arg and Cys, in any of which cases the .epsilon.-amino group of Lys.sup.B29 has a lipophilic substituent; and any Zn.sup.2+ complexes thereof with the proviso that when B30 is Thr or Ala and A21 and B3 are both Asn, and Phe.sup.B1 is present, then the insulin derivative is always present as a Zn.sup.2+ complex.

    摘要翻译： 本发明涉及延长的人胰岛素衍生物，其中A21和B3氨基酸残基独立地是可由遗传密码编码的除Lys，Arg和Cys之外的任何氨基酸残基; PheB1可能被删除; B30氨基酸残基是（a）具有10至24个碳原子的非可编码的亲脂性氨基酸，在这种情况下，具有至多5个碳原子的羧酸的酰基与ε-氨基的ε-氨基结合 LysB29; 或（b）B30氨基酸残基被缺失或者是任何氨基酸残基，其可以通过除Lys，Arg和Cys之外的遗传密码进行编码，在这些情况下，LysB29的ε-氨基具有亲脂性取代基; 和任何Zn 2+配合物，条件是当B30是Thr或Ala，A21和B3都是Asn和PheB1时，胰岛素衍生物总是作为Zn 2+络合物存在。

    DNA sequences encoding insulin precursors and methods of production
    39.
    发明授权
    DNA sequences encoding insulin precursors and methods of production 失效
    编码胰岛素前体的DNA序列和生产方法

    公开(公告)号：US5324641A

    公开(公告)日：1994-06-28

    申请号：US952696

    申请日：1992-09-23

    申请人： Ib Jonassen ,  Ib G. Clausen ,  Ejner B. Jensen ,  Allan Svendsen

    发明人： Ib Jonassen ,  Ib G. Clausen ,  Ejner B. Jensen ,  Allan Svendsen

    IPC分类号： C12N15/09 ,  A61K38/00 ,  C07K14/575 ,  C07K14/62 ,  C12N15/17 ,  C12P21/02 ,  C12R1/865 ,  C12N15/00 ,  C12N1/08 ,  C12P21/06

    CPC分类号： C07K14/62 ,  A61K38/00

    摘要： DNA molecule and process for producing insulin precursors having the formula B(1-29) -H.sub.1 --X.sub.2 --Y.sub.2 --Y.sub.1 --A(1-21), wherein B(1-29) are the 29 first amino acid residues of the B chain of human insulin starting from the N-terminus, A(1-21) are the 21 amino acid residues of the A chain of human insulin, X.sub.1 represents a peptide bond or one naturally-occurring alpha-amino acid acid residues, X.sub.2 represents Glu or Asp, and Y.sub.1 and Y.sub.2 each represent Lys or Arg, the positions A6 and A11, A7 and B7 and A20 and B19, respectively, are connected through sulphur bridges, and, if desired, one or more of the glutamic acid residues in positions A4, A17, B13 and B21 are substituted by another naturally-occurring alpha-amino acid residue having an uncharged side chain, are provided. The insulin precursors are prepared by culturing a yeast strain transformed with a replicable plasmid comprising a DNA sequence encoding an insulin precursor of the above formula in a suitable medium and isolating the insulin precursor thus formed from the culture medium. The insulin precursor can be converted into human insulin or insulin analogues by enzymatic treatment in a manner known per se.

    摘要翻译： DNA分子和具有式B（1-29）-H1-X2-Y2-Y1-A（1-21）的胰岛素前体的制备方法，其中B（1-29）是B的第一个氨基酸残基 A（1-21）是人胰岛素A链的21个氨基酸残基，X1表示肽键或一个天然存在的α-氨基酸酸残基，X2表示 Glu或Asp，Y1和Y2各自表示Lys或Arg，位置A6和A11，A7和B7和A20和B19分别通过硫桥连接，如果需要，一个或多个谷氨酸残基在 位置A4，A17，B13和B21被另一个具有不带电侧链的天然存在的α-氨基酸残基所取代。 通过在合适的培养基中培养用包含编码上式胰岛素前体的DNA序列的可复制质粒转化的酵母菌株并分离由培养基形成的胰岛素前体来制备胰岛素前体。 胰岛素前体可以通过本身已知的方式通过酶处理转化为人胰岛素或胰岛素类似物。