公开(公告)号：US20110171363A1

公开(公告)日：2011-07-14

申请号：US13052272

申请日：2011-03-21

申请人： Manabu Komatsu ,  Hiroyuki Hashimoto

发明人： Manabu Komatsu ,  Hiroyuki Hashimoto

IPC分类号： G01N23/00 ,  G01N1/28 ,  G01N23/22 ,  B05D1/02

CPC分类号： H01J49/10 ,  B01L3/5085 ,  H01J49/40 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/25 ,  Y10T436/2575

摘要： A test specimen that has one or more chemical substances fixed to prescribed plural independent positions on a substrate, and the quantities of the chemical substances fixed at the respective prescribed positions are the total of integer multiples of existence quantity units defined for the respective chemical substances in the range from 1 amol to 1 nmol (excluding the case in which the total quantity is zero).

摘要翻译： 将具有固定在基板上规定的多个独立位置的一种以上的化学物质的试样和固定在各规定位置的化学物质的量作为对各化学物质定义的存在量单位的整数倍的总和 范围从1 amol到1 nmol（不包括总量为零的情况）。

公开(公告)号：US20080277576A1

公开(公告)日：2008-11-13

申请号：US12117527

申请日：2008-05-08

申请人： Manabu Komatsu ,  Hiroyuki Hashimoto

发明人： Manabu Komatsu ,  Hiroyuki Hashimoto

IPC分类号： H01J49/00

CPC分类号： H01J49/142

摘要： A time-of-flight secondary ion mass spectrometer comprises an ion source which generates cluster ions each comprised of two or more atoms, a pulsing mechanism which pulses the cluster ions, a selecting mechanism which selects ions having a specific mass number from the pulsed cluster ions and passes the selected ions in an ON state of the selecting mechanism, and, passes the pulsed cluster ions without the selecting in an OFF state of the selecting mechanism, and a time-of-flight mass spectrometric unit which measures a mass spectrum of secondary ions generated from a sample using a difference in time of flight when the sample is irradiated with the ions passed through the selecting mechanism.

摘要翻译： 飞行时间二次离子质谱仪包括产生每个由两个或多个原子构成的簇离子的离子源，脉冲簇离子的脉冲机制，从脉冲簇选择具有特定质量数的离子的选择机制 离子，并将所选择的离子通过选择机构的导通状态，并且在选择机构的O​​FF状态下通过脉冲簇离子而不选择，并且测量飞行时间质谱单位， 当使用通过选择机构的离子照射样品时，使用飞行时间差来自样品产生的二次离子。

公开(公告)号：US20080268497A1

公开(公告)日：2008-10-30

申请号：US12098822

申请日：2008-04-07

申请人： Hiroyuki Hashimoto ,  Manabu Komatsu ,  Satoru Hatakeyama

发明人： Hiroyuki Hashimoto ,  Manabu Komatsu ,  Satoru Hatakeyama

IPC分类号： G01N1/30 ,  C12Q1/00

CPC分类号： G01N33/6848 ,  B82Y15/00 ,  G01N33/54313

摘要： The present invention intends to provide a high sensitivity method for identifying the type of a protein contained in each cell with a diameter of ten to several tens μm and a sample treatment solution required for it. The above is achieved by three steps of (1) treating a slice of a diseased tissue with an aqueous solution containing gold particles and a digestive enzyme, and digesting restrictively a protein of interest, (2) measuring a 2-dimensional distribution of fragmented peptides by TOF-SIMS, and (3) visualizing a 2-dimensional distribution of the target protein in the slice of the diseased tissue using the results of the proteome analysis and by means of a numerical analysis.

摘要翻译： 本发明旨在提供用于鉴定直径为10至数十微米的每个电池中所含的蛋白质的类型的高灵敏度方法和所需的样品处理溶液。 上述通过以下三个步骤实现：（1）用含有金颗粒和消化酶的水溶液处理患病组织的切片，并限制性地消化目的蛋白质，（2）测量片段肽的2维分布 通过TOF-SIMS，以及（3）使用蛋白质组分析的结果并通过数值分析来显现病变组织切片中靶蛋白的二维分布。

公开(公告)号：US08623656B2

公开(公告)日：2014-01-07

申请号：US13052272

申请日：2011-03-21

申请人： Manabu Komatsu ,  Hiroyuki Hashimoto

发明人： Manabu Komatsu ,  Hiroyuki Hashimoto

IPC分类号： G01N35/00 ,  G01N1/00 ,  G01N15/06 ,  G01N33/48

CPC分类号： H01J49/10 ,  B01L3/5085 ,  H01J49/40 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/25 ,  Y10T436/2575

摘要： A test specimen that has one or more chemical substances fixed to prescribed plural independent positions on a substrate, and the quantities of the chemical substances fixed at the respective prescribed positions are the total of integer multiples of existence quantity units defined for the respective chemical substances in the range from 1 amol to 1 nmol (excluding the case in which the total quantity is zero).

摘要翻译： 将具有固定在基板上规定的多个独立位置的一种以上的化学物质的试样和固定在各规定位置的化学物质的量作为对各化学物质定义的存在量单位的整数倍的总和 范围从1 amol到1 nmol（不包括总量为零的情况）。

公开(公告)号：US20120298859A1

公开(公告)日：2012-11-29

申请号：US13575600

申请日：2011-01-31

申请人： Koichi Tanji ,  Manabu Komatsu ,  Hiroyuki Hashimoto

发明人： Koichi Tanji ,  Manabu Komatsu ,  Hiroyuki Hashimoto

IPC分类号： H01J49/26

CPC分类号： H01J49/0036 ,  H01J49/0004

摘要： A more effective noise reduction method is provided. In the method, when mass spectrum information having a spatial distribution is processed, the whole data is taken as three-dimensional data (positional information is stored in an xy plane, and spectral information is stored along a z-axis direction), and three-dimensional wavelet noise reduction is performed by applying preferable basis functions to a spectral direction and a peak distribution direction (in-plane direction).

摘要翻译： 提供了更有效的降噪方法。 在该方法中，当处理具有空间分布的质谱信息时，将整个数据作为三维数据（位置信息存储在xy平面中，并且光谱信息沿z轴方向存储），并且三个 通过对光谱方向和峰分布方向（面内方向）应用优选的基函数来进行维小波噪声降低。

公开(公告)号：US20110315871A1

公开(公告)日：2011-12-29

申请号：US13255094

申请日：2010-03-30

申请人： Manabu Komatsu ,  Hiroyuki Hashimoto

发明人： Manabu Komatsu ,  Hiroyuki Hashimoto

IPC分类号： H01J49/26 ,  H01J49/40

CPC分类号： G06F19/26 ,  G01N33/6848 ,  H01J49/0004 ,  Y10T436/24

摘要： An object of the present invention is to provide a method of comprehensively visualizing a constituent in tumor tissue or the like at a cellular level.The present invention provides a method of forming a two-dimensional distribution image of a target constituent based on information on the mass of constituents of the tissue section wherein, as the internal standard material, any one of actin, tubulin and GAPDH is used in the intracellular region, one of histone and nucleic acid is used in the nuclear region, and one of albumin and cytokine is used in the extracellular region.

摘要翻译： 本发明的目的是提供一种在细胞水平上全面显示肿瘤组织等中的成分的方法。 本发明提供一种基于组织部位成分质量的信息形成目标成分的二维分布图像的方法，其中作为内标物质，使用肌动蛋白，微管蛋白和GAPDH中的任一种 细胞内区域，组蛋白和核酸之一用于核区域，白蛋白和细胞因子之一用于细胞外区域。

公开(公告)号：US20090308141A1

公开(公告)日：2009-12-17

申请号：US12542201

申请日：2009-08-17

申请人： Manabu Komatsu ,  Hiroyuki Hashimoto

发明人： Manabu Komatsu ,  Hiroyuki Hashimoto

IPC分类号： G01N1/00

CPC分类号： H01J49/10 ,  B01L3/5085 ,  H01J49/40 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/25 ,  Y10T436/2575

摘要： A test specimen is provided which has one or more chemical substances fixed to prescribed plural independent positions on a substrate, and the quantities of the chemical substances fixed at the respective prescribed positions are the total of integer multiples of existence quantity units defined for the respective chemical substances in the range from 1 amol to 1 nmol (excluding the case in which the total quantity is zero).

摘要翻译： 提供了一种试样，其具有在基板上固定为规定的多个独立位置的一种或多种化学物质，并且在各规定位置固定的化学物质的量为为各化学物质定义的存在量单位的整数倍的总和 物质在1〜1nmol范围内（不包括总量为零的情况）。

公开(公告)号：US08384021B2

公开(公告)日：2013-02-26

申请号：US13255094

申请日：2010-03-30

申请人： Manabu Komatsu ,  Hiroyuki Hashimoto

发明人： Manabu Komatsu ,  Hiroyuki Hashimoto

IPC分类号： H01J49/00 ,  G01N33/00 ,  G01N24/00

CPC分类号： G06F19/26 ,  G01N33/6848 ,  H01J49/0004 ,  Y10T436/24

摘要： An object of the present invention is to provide a method of comprehensively visualizing a constituent in tumor tissue or the like at a cellular level.The present invention provides a method of forming a two-dimensional distribution image of a target constituent based on information on the mass of constituents of the tissue section wherein, as the internal standard material, any one of actin, tubulin and GAPDH is used in the intracellular region, one of histone and nucleic acid is used in the nuclear region, and one of albumin and cytokine is used in the extracellular region.

公开(公告)号：US20120301968A1

公开(公告)日：2012-11-29

申请号：US13576124

申请日：2011-03-28

申请人： Jumpei Naito ,  Shuya Satoh ,  Manabu Komatsu ,  Hiroyuki Hashimoto

发明人： Jumpei Naito ,  Shuya Satoh ,  Manabu Komatsu ,  Hiroyuki Hashimoto

IPC分类号： G01N27/62

CPC分类号： G01N33/6851 ,  H01J49/00 ,  Y10T436/182

摘要： The problem to be solved by the present invention is to provide a method for treating a polypeptide including a cysteine residue for enhancing the sensitivity of detection thereof in mass spectrometry using charged particles. A mass spectrometry method for a polypeptide including a cysteine residue is provided which includes treating the polypeptide including a cysteine residue with a cyanation agent, treating the cyanated polypeptide with a base, and then desorption ionizing the base-treated polypeptides using charged particles for mass spectrometry.

摘要翻译： 本发明要解决的问题是提供一种用于在使用带电粒子的质谱法中治疗包含半胱氨酸残基的多肽以提高其检测灵敏度的方法。 提供了包括半胱氨酸残基的多肽的质谱法，其包括用氰化剂处理包含半胱氨酸残基的多肽，用碱处理氰化多肽，然后使用带电粒子对基质处理的多肽进行解吸，用于质谱法 。

公开(公告)号：US07714280B2

公开(公告)日：2010-05-11

申请号：US12117527

申请日：2008-05-08

申请人： Manabu Komatsu ,  Hiroyuki Hashimoto

发明人： Manabu Komatsu ,  Hiroyuki Hashimoto

IPC分类号： H01J49/44

CPC分类号： H01J49/142

摘要： A time-of-flight secondary ion mass spectrometer comprises an ion source which generates cluster ions each comprised of two or more atoms, a pulsing mechanism which pulses the cluster ions, a selecting mechanism which selects ions having a specific mass number from the pulsed cluster ions and passes the selected ions in an ON state of the selecting mechanism, and, passes the pulsed cluster ions without the selecting in an OFF state of the selecting mechanism, and a time-of-flight mass spectrometric unit which measures a mass spectrum of secondary ions generated from a sample using a difference in time of flight when the sample is irradiated with the ions passed through the selecting mechanism.

摘要翻译： 飞行时间二次离子质谱仪包括产生每个由两个或多个原子构成的簇离子的离子源，脉冲簇离子的脉冲机制，从脉冲簇选择具有特定质量数的离子的选择机制 离子，并将所选择的离子通过选择机构的导通状态，并且在选择机构的O​​FF状态下通过脉冲簇离子而不选择，并且测量飞行时间质谱单位， 当使用通过选择机构的离子照射样品时，使用飞行时间差来自样品产生的二次离子。