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    Genes encoding carotenoid compounds
    33.
    发明申请
    Genes encoding carotenoid compounds 失效
    编码类胡萝卜素的基因

    公开(公告)号：US20050014219A1

    公开(公告)日：2005-01-20

    申请号：US10810733

    申请日：2004-03-26

    申请人： Qiong Cheng ,  Luan Tao ,  Natalia Sedkova

    发明人： Qiong Cheng ,  Luan Tao ,  Natalia Sedkova

    IPC分类号： C07H21/04 ,  C12N1/21 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/90 ,  C12N15/52 ,  C12N15/74 ,  C12P7/26 ,  C12P23/00

    CPC分类号： C12N9/001 ,  C07H21/04 ,  C12N9/00 ,  C12N9/0004 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/1051 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12P23/00 ,  C12Y205/01029 ,  Y02E50/343

    摘要： A carotenogenic biosynthetic gene cluster has been isolated from Panteoa stewartii strain DC413, wherein the genetic organization of the cluster is crtE-idi-crt)(-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), zeaxanthin glucosyl transferase (CrtX), lycopene cyclase (CrtY), phytoene desaturase (Crtl), phytoene synthase (CrtB), and β-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.

    摘要翻译： 从Panteoa stewartii菌株DC413中分离出一种致病基因生物合成基因簇，其中簇的遗传组织为crtE-idi-crt（-crtY-crtI-crtB-crtZ），该簇内包含的基因编码geranylgeranyl pyrophosphate（GGPP） 合成酶（CrtE），异戊烯焦磷酸异构酶（Idi），玉米黄质葡萄糖基转移酶（CrtX），番茄红素环化酶（CrtY），八氢番茄红素去饱和酶（Crtl），八氢番茄红素合酶（CrtB）和β-胡萝卜素羟化酶（CrtZ） 公开了基因及其产物可用于将焦磷酸法呢基转化为类胡萝卜素。含有这些DNA片段的载体，含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

    Broad host range pBBR1-based plasmid mutant derivatives having altered plasmid copy number
    36.
    发明授权
    Broad host range pBBR1-based plasmid mutant derivatives having altered plasmid copy number 失效
    具有改变的质粒拷贝数的基于pBBR1的质粒突变体衍生物

    公开(公告)号：US07247467B2

    公开(公告)日：2007-07-24

    申请号：US10940052

    申请日：2004-09-14

    申请人： Qiong Cheng ,  Luan Tao

    发明人： Qiong Cheng ,  Luan Tao

    IPC分类号： C12N1/21

    CPC分类号： C12N15/70 ,  C12N15/74

    摘要： The present invention describes a mutant plasmid replication control region having the ability to convey a phenotype of altered plasmid copy number to the plasmid on which it resides. The mutant replication control region is based on a similar region isolated from the pBBR1 plasmid family. Plasmids containing this replication control region cannot be classed as belonging to any known incompatibility group and thus may co-exist with a broad range of other plasmids in a single host.

    摘要翻译： 本发明描述了具有将改变的质粒拷贝数表型转移到其所在质粒上的突变质粒复制控制区。 突变体复制控制区基于与pBBR1质粒家族分离的相似区域。 含有该复制控制区的质粒不能归类为任何已知的不相容性基团，因此可以与单个宿主中的宽范围的其他质粒共存。

    Carotenoid hydroxylase enzymes
    39.
    发明申请
    Carotenoid hydroxylase enzymes 有权
    类胡萝卜素羟化酶

    公开(公告)号：US20060035312A1

    公开(公告)日：2006-02-16

    申请号：US11200394

    申请日：2005-08-09

    申请人： Qiong Cheng ,  Luan Tao ,  Natalia Sedkova

    发明人： Qiong Cheng ,  Luan Tao ,  Natalia Sedkova

    IPC分类号： C12P23/00 ,  C07H21/04 ,  C12N9/02 ,  C12N1/18 ,  C12N15/74 ,  C12N1/16

    CPC分类号： C12N9/0071 ,  C12N9/0004 ,  C12N15/52 ,  C12P23/00

    摘要： A novel CrtZ carotenoid hydroxylase, isolated from Brevundimonas vesicularis DC263, is provided that is useful for the production of hydroxylated carotenoids. Additionally, a previously identified hypothetical protein from Novosphingobium aromaticivorans has found to have carotenoid hydroxylase activity. Both hydroxylase genes exhibit low homology in comparison to other CrtZ hydroxylases previously reported. Expression of the hydroxylases in heterologous host cells enabled production of hydroxylated carotenoids.

    摘要翻译： 提供了从Brevundimonas vesicularis DC263中分离的新型CrtZ类胡萝卜素羟化酶，其可用于生产羟基化类胡萝卜素。 另外，以前鉴定出来自新西伯替尼葡萄球菌的假想蛋白质已经发现具有类胡萝卜素羟化酶活性。 与以前报道的其他CrtZ羟化酶相比，羟化酶基因都表现出低的同源性。 异源宿主细胞中羟化酶的表达使得能够产生羟化的类胡萝卜素。