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    Method of determination of gene function
    5.
    发明授权
    Method of determination of gene function 失效
    测定基因功能的方法

    公开(公告)号：US06673567B2

    公开(公告)日：2004-01-06

    申请号：US09808658

    申请日：2001-03-15

    申请人： Pamela L. Sharpe ,  Vasantha Nagarajan

    发明人： Pamela L. Sharpe ,  Vasantha Nagarajan

    IPC分类号： C12Q100

    CPC分类号： C12N15/1082 ,  C12N15/1079 ,  C12Q1/6897

    摘要： The present method is useful for the identification of genes, ORF's and other nucleic acid molecules which are essential for the expression of a specific phenotype in microorganisms. The method employs In vitro transposition in conjunction with an chromosomal integration vector containing a specific gene or genetic element whose function is unknown. Subsequent transformation of a recombination proficient host with the vector and growth first under non-integrating conditions and then under integrating conditions, followed by a selection screen for either single or double crossover events results in transformants that may be subjected to phenotypic screens to determine gene function.

    摘要翻译： 本发明方法可用于鉴定在微生物中表达特定表型所必需的基因ORF和其它核酸分子。 该方法使用体外转座与包含功能未知的特定基因或遗传元件的染色体整合载体。 随后，在非整合条件下，然后在整合条件下，首先用载体和生长进行重组熟练宿主的转化，然后在单一或双交换事件的选择筛选中导致可以进行表型筛选以确定基因功能的转化体 。

    Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
    6.
    发明授权
    Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production 有权
    优化的解脂耶氏酵母菌株用于高二十碳五烯酸生产

    公开(公告)号：US08637298B2

    公开(公告)日：2014-01-28

    申请号：US12814815

    申请日：2010-06-14

    申请人： Seung-Pyo Hong ,  Pamela L. Sharpe ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    发明人： Seung-Pyo Hong ,  Pamela L. Sharpe ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    IPC分类号： C12N1/00

    CPC分类号： C12N9/1029 ,  A21D2/165 ,  A23K20/158 ,  C12N9/00 ,  C12N9/13 ,  C12N9/93 ,  C12P7/6427 ,  C12P7/6472 ,  C12P7/6481

    摘要： Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The expression of at least one peroxisome biogenesis factor protein is down-regulated. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

    摘要翻译： 描述了能够产生包含大于50重量％的二十碳五烯酸[“EPA”]的油的油质酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，其是以总脂肪酸的重量百分比测量的ω-3多不饱和脂肪酸[“％ TFAs“]，并且具有至少3.1％的EPA％TFA与比率为TFA的亚油酸的比率。 除了其他异源Delta9延伸酶，Delta8去饱和酶，Δ5去饱和酶，Δ17去饱和酶，Δ12去饱和酶，C16 / 18延长酶和任选的过表达二酰基甘油胆碱磷酸转移酶，丙二酰辅酶A，这些菌株过表达至少一种Δ9延伸酶/Δδ去饱和酶多酶 合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。 至少一种过氧化物酶体生物因子蛋白的表达下调。 要求在所述宿主细胞内生产EPA的方法，从细胞获得的油及其产物。

    High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica
    8.
    发明授权
    High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica 有权
    来自改良的解脂耶氏酵母菌株的高二十碳五烯酸油

    公开(公告)号：US08399226B2

    公开(公告)日：2013-03-19

    申请号：US12814880

    申请日：2010-06-14

    申请人： Seung-Pyo Hong ,  Pamela L. Sharpe ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    发明人： Seung-Pyo Hong ,  Pamela L. Sharpe ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    IPC分类号： C12P7/64 ,  A23D9/00

    CPC分类号： C12N9/1029 ,  A21D2/165 ,  A23K20/158 ,  C12N9/00 ,  C12N9/13 ,  C12N9/93 ,  C12P7/6427 ,  C12P7/6472 ,  C12P7/6481

    摘要： Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

    摘要翻译： 描述了能够产生包含大于50重量％的二十碳五烯酸[EPA]，ω-3多不饱和脂肪酸的脂肪酵母解脂耶氏酵母的工程菌株，以总脂肪酸的重量％[％TFA]和 具有至少3.1％的EPA％TFA与亚油酸的比例，以％TFA测定。 除了其他异源＆Dgr .9延伸酶，Dgr。8去饱和酶，Dgr。5去饱和酶，Dgr 17脱饱和酶，Dgr。12去饱和酶，C16外，这些菌株过度表达至少一种Dgr 9延伸酶/ / 18延伸酶和任选的过表达二酰基甘油胆碱磷酸转移酶，丙二酰辅酶A合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。 该菌株具有至少一种过氧化物酶体生物发生因子蛋白质敲除。 要求在所述宿主细胞内生产EPA的方法，从细胞获得的油及其产物。

    Method to increase carotenoid production in a microbial host cell by down-regulating glycogen synthase
    9.
    发明授权
    Method to increase carotenoid production in a microbial host cell by down-regulating glycogen synthase 失效
    通过下调糖原合成酶来增加微生物宿主细胞中类胡萝卜素产生的方法

    公开(公告)号：US07504236B2

    公开(公告)日：2009-03-17

    申请号：US11732165

    申请日：2007-04-02

    申请人： Edward S. Miller, Jr. ,  James M. Odom ,  Pamela L. Sharpe

    发明人： Edward S. Miller, Jr. ,  James M. Odom ,  Pamela L. Sharpe

    IPC分类号： C12P23/00 ,  C12N1/13

    CPC分类号： C12N9/1051 ,  C12P23/00

    摘要： A method to increase carotenoid production in carotenogenic microbial host cells is provided by down-regulating or disrupting glycogen synthesis. Disruption of glycogen synthase activity in a carotenogenic microbial host cell significantly increased carotenoid production. Carotenogenic microorganisms are also provided that have been optimized for the production of carotenoid compounds through the down-regulation and/or disruption of glycogen synthase activity.

    摘要翻译： 通过下调或破坏糖原合成来提供在胡萝卜素微生物宿主细胞中增加类胡萝卜素产生的方法。 致癌微生物宿主细胞中糖原合成酶活性的破坏显着增加类胡萝卜素的产生。 还提供了通过下调和/或破坏糖原合成酶活性而优化用于生产类胡萝卜素化合物的致细性微生物。

    CAROTENOID PRODUCTION IN A RECOMBINANT OLEAGINOUS YEAST
    10.
    发明申请
    CAROTENOID PRODUCTION IN A RECOMBINANT OLEAGINOUS YEAST 有权
    在重组油脂中生产碳酸钙

    公开(公告)号：US20120142082A1

    公开(公告)日：2012-06-07

    申请号：US11952243

    申请日：2007-12-07

    申请人： Pamela L. Sharpe ,  Rick W. Ye ,  Quinn Qun Zhu

    发明人： Pamela L. Sharpe ,  Rick W. Ye ,  Quinn Qun Zhu

    IPC分类号： C12N1/19 ,  A23K1/18 ,  A23L1/27 ,  C12P23/00 ,  A23K1/00

    CPC分类号： C12P23/00 ,  C07C403/24 ,  C09B61/00 ,  C12P7/6427 ,  C12P7/6472

    摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing carotenoids (e.g., β-carotene, lycopene, lutein, zeaxanthin, canthaxanthin, astaxanthin) are provided. The strains may also be engineered to co-produce at least one ω-3/ω-6 polyunsaturated fatty acid and/or at least one additional antioxidant. Methods of using the carotenoid products obtained (e.g., biomass and/or pigmented oils) in food and feed applications are also provided.

    摘要翻译： 提供能够产生类胡萝卜素（例如，胡萝卜素，番茄红素，叶黄素，玉米黄质，角黄素，虾青素）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 菌株也可以被工程化以共同产生至少一种ω-3 /ω-6多不饱和脂肪酸和/或至少一种另外的抗氧化剂。 还提供了在食品和饲料应用中使用获得的类胡萝卜素产物（例如生物质和/或着色油）的方法。