Genes encoding carotenoid compounds
    2.
    发明授权
    Genes encoding carotenoid compounds 失效
    编码类胡萝卜素的基因

    公开(公告)号:US06929928B2

    公开(公告)日:2005-08-16

    申请号:US10808807

    申请日:2004-03-24

    摘要: A unique carotenogenic biosynthetic gene cluster has been isolated from Panteoa agglomerans strain DC404, wherein the genetic organization of the cluster is crtE-idi-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), lycopene cyclase (CrtY), phytoene desaturase (CrtI), phytoene synthase (CrtB), and β-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.

    摘要翻译: 已经从成团Panthena菌株DC404中分离出独特的致病基因生物合成基因簇,其中簇的遗传组织为crtE-idi-crtY-crtI-crtB-crtZ。 包含在该簇内的基因编码er牛儿基焦磷酸(GGPP)合成酶(CrtE),异戊烯焦磷酸异构酶(Idi),番茄红素环化酶(CrtY),八氢番茄红素去饱和酶(CrtI),八氢番茄红素合酶(CrtB)和β-胡萝卜素羟化酶(CrtZ) 。 基因簇,基因及其产物可用于将焦磷酸法呢基转化成类胡萝卜素。 公开了含有这些DNA片段的载体,含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

    Genes encoding carotenoid compounds
    3.
    发明授权
    Genes encoding carotenoid compounds 失效
    编码类胡萝卜素的基因

    公开(公告)号:US07070952B2

    公开(公告)日:2006-07-04

    申请号:US10804677

    申请日:2004-03-19

    摘要: Genes have been isolated from Pectobacterium cypripedii encoding geranylgeranyl pyrophosphate (GGPP) synthase (CrtE), phytoene synthase (CrtB), phytoene desaturase (Crtl), lycopene cyclase (CrtY), β-carotene hydroxylase (CrtZ), and zeaxanthin glucosyl transferase (CrtX) activity. The genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.

    摘要翻译: 已经从编码香叶基香叶基焦磷酸(GGPP)合成酶(CrtE),八氢番茄红素合酶(CrtB),八氢番茄红素去饱和酶(Crt1),番茄红素环化酶(CrtY),β-胡萝卜素羟化酶(CrtZ)和玉米黄质葡萄糖基转移酶(CrtX )活动。 这些基因及其产物可用于将焦磷酸法呢基转化成类胡萝卜素。 公开了含有这些DNA片段的载体,含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

    Carotenoid hydroxylase enzymes
    4.
    发明申请
    Carotenoid hydroxylase enzymes 有权
    类胡萝卜素羟化酶

    公开(公告)号:US20060035312A1

    公开(公告)日:2006-02-16

    申请号:US11200394

    申请日:2005-08-09

    摘要: A novel CrtZ carotenoid hydroxylase, isolated from Brevundimonas vesicularis DC263, is provided that is useful for the production of hydroxylated carotenoids. Additionally, a previously identified hypothetical protein from Novosphingobium aromaticivorans has found to have carotenoid hydroxylase activity. Both hydroxylase genes exhibit low homology in comparison to other CrtZ hydroxylases previously reported. Expression of the hydroxylases in heterologous host cells enabled production of hydroxylated carotenoids.

    摘要翻译: 提供了从Brevundimonas vesicularis DC263中分离的新型CrtZ类胡萝卜素羟化酶,其可用于生产羟基化类胡萝卜素。 另外,以前鉴定出来自新西伯替尼葡萄球菌的假想蛋白质已经发现具有类胡萝卜素羟化酶活性。 与以前报道的其他CrtZ羟化酶相比,羟化酶基因都表现出低的同源性。 异源宿主细胞中羟化酶的表达使得能够产生羟化的类胡萝卜素。

    Genes of strain DC413 encoding enzymes involved in biosynthesis of carotenoid compounds
    6.
    发明授权
    Genes of strain DC413 encoding enzymes involved in biosynthesis of carotenoid compounds 失效
    编码参与类胡萝卜素化合物生物合成的酶的DC413菌株基因

    公开(公告)号:US07288387B2

    公开(公告)日:2007-10-30

    申请号:US10810733

    申请日:2004-03-26

    摘要: A carotenogenic biosynthetic gene cluster has been isolated from Panteoa stewartii strain DC413, wherein the genetic organization of the cluster is crtE-idi-crtX-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), zeaxanthin glucosyl transferase (CrtX), lycopene cyclase (CrtY), phytoene desaturase (CrtI), phytoene synthase (CrtB), and β-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.

    摘要翻译: 已经从Panteoa stewartii菌株DC413中分离出了一种类胡萝卜素生物合成基因簇,其中簇的遗传组织为crtE-idi-crtX-crtY-crtI-crtB-crtZ。 包含在该簇内的基因编码er牛儿基焦磷酸(GGPP)合成酶(CrtE),异戊烯焦磷酸异构酶(Idi),玉米黄质葡萄糖基转移酶(CrtX),番茄红素环化酶(CrtY),八氢番茄红素去饱和酶(CrtI),八氢番茄红素合酶(CrtB) β-胡萝卜素羟化酶(CrtZ)。 基因簇,基因及其产物可用于将焦磷酸法呢基转化为类胡萝卜素。 公开了含有这些DNA片段的载体,含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

    Genes encoding carotenoid compounds
    8.
    发明申请
    Genes encoding carotenoid compounds 失效
    编码类胡萝卜素的基因

    公开(公告)号:US20050014219A1

    公开(公告)日:2005-01-20

    申请号:US10810733

    申请日:2004-03-26

    摘要: A carotenogenic biosynthetic gene cluster has been isolated from Panteoa stewartii strain DC413, wherein the genetic organization of the cluster is crtE-idi-crt)(-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), zeaxanthin glucosyl transferase (CrtX), lycopene cyclase (CrtY), phytoene desaturase (Crtl), phytoene synthase (CrtB), and β-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.

    摘要翻译: 从Panteoa stewartii菌株DC413中分离出一种致病基因生物合成基因簇,其中簇的遗传组织为crtE-idi-crt(-crtY-crtI-crtB-crtZ),该簇内包含的基因编码geranylgeranyl pyrophosphate(GGPP) 合成酶(CrtE),异戊烯焦磷酸异构酶(Idi),玉米黄质葡萄糖基转移酶(CrtX),番茄红素环化酶(CrtY),八氢番茄红素去饱和酶(Crtl),八氢番茄红素合酶(CrtB)和β-胡萝卜素羟化酶(CrtZ) 公开了基因及其产物可用于将焦磷酸法呢基转化为类胡萝卜素。含有这些DNA片段的载体,含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。