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59 条记录 (耗时 0.097 秒)

    Neutralizing anti-influenza A virus antibodies and uses thereof
    31.
    发明授权
    Neutralizing anti-influenza A virus antibodies and uses thereof 有权

    公开(公告)号：US10815294B2

    公开(公告)日：2020-10-27

    申请号：US16178497

    申请日：2018-11-01

    申请人： INSTITUTE FOR RESEARCH IN BIOMEDICINE

    发明人： Antonio Lanzavecchia

    IPC分类号： C07K16/10 ,  C07K14/005 ,  G01N33/569 ,  A61K39/00

    摘要： The invention relates to antibodies, and antigen binding fragments thereof, that specifically bind to an epitope in the stem region of an influenza A hemagglutinin trimer and neutralize a group 1 subtype and a group 2 subtype of influenza A virus. The invention also relates to nucleic acids that encode, immortalized B cells and cultured single plasma cells that produce, and to epitopes that bind to such antibodies and antibody fragments. In addition, the invention relates to the use of the antibodies, antibody fragments, and epitopes in screening methods as well as in the diagnosis, treatment and prevention of influenza A virus infection.

    Multispecific anti GM-CSF antibodies
    33.
    发明授权
    Multispecific anti GM-CSF antibodies 有权

    公开(公告)号：US10526404B2

    公开(公告)日：2020-01-07

    申请号：US15568325

    申请日：2015-04-29

    申请人： INSTITUTE FOR RESEARCH IN BIOMEDICINE

    发明人： Davide Corti ,  Antonio Lanzavecchia ,  Luca Piccoli

    IPC分类号： C07K16/24 ,  C07K16/46 ,  A61K39/00

    摘要： The present invention provides multispecific antibodies, and antigen binding fragments thereof, that potently neutralize a cytokine and that may thus be useful in the prevention and/or treatment of inflammatory and/or autoimmune diseases. In particular, the present invention provides a multispecific antibody, or an antigen binding fragment thereof, comprising at least two different domains specifically binding to at least two different, non-overlapping sites in a cytokine and an Fc moiety. The invention also relates to nucleic acids that encode such antibodies and antibody fragments and immortalized B cells and cultured plasma cells that produce such antibodies and antibody fragments. In addition, the invention relates to the use of the antibodies and antibody fragments of the invention in screening methods as well as in the diagnosis, prophylaxis and treatment of inflammatory and/or autoimmune diseases.

    MONOCLONAL ANTIBODY PRODUCTION BY EBV TRANSFORMATION OF B CELLS
    36.
    发明申请
    MONOCLONAL ANTIBODY PRODUCTION BY EBV TRANSFORMATION OF B CELLS 审中-公开
    B细胞EBV转化的单克隆抗体生产

    公开(公告)号：US20160160258A1

    公开(公告)日：2016-06-09

    申请号：US15018688

    申请日：2016-02-08

    申请人： Institute For Research in Biomedicine

    发明人： Antonio Lanzavecchia

    IPC分类号： C12P21/00 ,  C07K16/10

    CPC分类号： C12P21/00 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/10 ,  C07K16/1027 ,  C07K16/1282 ,  C07K16/20 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/76 ,  C12N5/0635 ,  C12N2510/02 ,  C12N2510/04 ,  C12P21/005 ,  Y02A50/412 ,  Y02A50/466 ,  Y02A50/472 ,  Y02A50/58

    摘要： A method for producing a clone of an immortalised human B memory lymphocyte, comprising the step of transforming human B memory lymphocytes using Epstein Barr virus (EBV) in the presence of a polyclonal B cell activator. The method is particularly useful in a method for producing a clone of an immortalised human B memory lymphocyte capable of producing a human monoclonal antibody with a desired antigen specificity, comprising the steps of: (i) selecting and isolating a human memory B lymphocyte subpopulation; (ii) transforming the subpopulation with Epstein Barr virus (EBV) in the presence of a polyclonal B cell activator; (iii) screening the culture supernatant for antigen specificity; and (iv) isolating an immortalised human B memory lymphocyte clone capable of producing a human monoclonal antibody having the desired antigen specificity.

    摘要翻译： 一种生产永生化人B记忆淋巴细胞克隆的方法，其包括在多克隆B细胞激活剂存在下使用爱泼斯坦巴尔病毒（EBV）转化人B记忆淋巴细胞的步骤。 该方法特别可用于生产能够产生具有所需抗原特异性的人单克隆抗体的永生化人B记忆淋巴细胞克隆的方法，包括以下步骤：（i）选择和分离人类记忆B淋巴细胞亚群; （ii）在多克隆B细胞激活剂存在下，用爱泼斯坦巴尔病毒（EBV）转化亚群; （iii）筛选培养上清液以获得抗原特异性; 和（iv）分离能够产生具有所需抗原特异性的人单克隆抗体的永生化人B记忆淋巴细胞克隆。

    MONOCLONAL ANTIBODY PRODUCTION BY EBV TRANSFORMATION OF B CELLS
    40.
    发明申请
    MONOCLONAL ANTIBODY PRODUCTION BY EBV TRANSFORMATION OF B CELLS 有权
    B细胞EBV转化的单克隆抗体生产

    公开(公告)号：US20140004123A1

    公开(公告)日：2014-01-02

    申请号：US13870804

    申请日：2013-04-25

    申请人： Institute For Research in Biomedicine

    发明人： Antonio Lanzavecchia

    IPC分类号： C07K16/10 ,  C12P21/00

    CPC分类号： C12P21/00 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/10 ,  C07K16/1027 ,  C07K16/1282 ,  C07K16/20 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/76 ,  C12N5/0635 ,  C12N2510/02 ,  C12N2510/04 ,  C12P21/005 ,  Y02A50/412 ,  Y02A50/466 ,  Y02A50/472

    摘要： A method for producing a clone of an immortalised human B memory lymphocyte, comprising the step of transforming human B memory lymphocytes using Epstein Barr virus (EBV) in the presence of a polyclonal B cell activator. The method is particularly useful in a method for producing a clone of an immortalised human B memory lymphocyte capable of producing a human monoclonal antibody with a desired antigen specificity, comprising the steps of: (i) selecting and isolating a human memory B lymphocyte subpopulation; (ii) transforming the subpopulation with Epstein Ban virus (EBV) in the presence of a polyclonal B cell activator; (iii) screening the culture supernatant for antigen specificity; and (iv) isolating an immortalised human B memory lymphocyte clone capable of producing a human monoclonal antibody having the desired antigen specificity.

    摘要翻译： 一种生产永生化人B记忆淋巴细胞克隆的方法，其包括在多克隆B细胞激活剂存在下使用爱泼斯坦巴尔病毒（EBV）转化人B记忆淋巴细胞的步骤。 该方法特别可用于生产能够产生具有所需抗原特异性的人单克隆抗体的永生化人B记忆淋巴细胞克隆的方法，包括以下步骤：（i）选择和分离人类记忆B淋巴细胞亚群; （ii）在存在多克隆B细胞激活剂的情况下用爱泼斯坦班病毒（EBV）转化亚群; （iii）筛选培养上清液以获得抗原特异性; 和（iv）分离能够产生具有所需抗原特异性的人单克隆抗体的永生化人B记忆淋巴细胞克隆。