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    Method for isolation of soluble polypeptides
    3.
    发明授权
    Method for isolation of soluble polypeptides 有权

    公开(公告)号：US11993643B2

    公开(公告)日：2024-05-28

    申请号：US17372966

    申请日：2021-07-12

    申请人： National Research Council of Canada

    发明人： Jamshid Tanha

    IPC分类号： C07K16/00 ,  C07K16/12 ,  C12N15/10 ,  G01N33/68

    CPC分类号： C07K16/005 ,  C07K16/1271 ,  C07K16/1282 ,  C12N15/1037 ,  G01N33/6845 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/92 ,  C07K2317/94

    摘要： Polypeptides with biophysical properties such as solubility, stability, high expression, monomericity, binding specificity or non-aggregation, including monomeric human heavy and light chain variable domains (VHs and VLs), are identified using a high throughput method for screening polypeptides, comprising the steps of obtaining a phage display library, allowing infection of a bacterial lawn by the library phage, and identifying phage which form larger than average plaques on the bacterial lawn. Sequences of monomeric human VHs and VLs are identified, which may be useful for immunotherapy or as diagnostic agents. Multimer complexes of human VHs and VLs are also identified. The VHs and VLs identified may be used to create further libraries for identifying additional polypeptides. Further, the VHs and VLs may be subjected to DNA shuffling to select for improved biophysical properties.

    METHOD OF PRODUCING ANTIBODIES FROM SINGLE PLASMA CELLS
    4.
    发明公开
    METHOD OF PRODUCING ANTIBODIES FROM SINGLE PLASMA CELLS 审中-公开

    公开(公告)号：US20240150446A1

    公开(公告)日：2024-05-09

    申请号：US18505543

    申请日：2023-11-09

    申请人： Daro Zist Azma Oxin

    发明人： Zohre BAZAZ ,  Masoume BAZAZ

    IPC分类号： C07K16/12 ,  C12N5/0781

    CPC分类号： C07K16/1282 ,  C12N5/0635 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/622 ,  C12N2500/25 ,  C12N2500/30 ,  C12N2501/999 ,  C12N2502/1358

    摘要： A method for producing an antibody fragment from one or more single plasma cells. The method comprises obtaining a plurality of plasma cells, preparing a plurality of modified cell culture substrates, seeding each respective plasma cell of the plurality of plasma cells on the each respective modified cell culture substrate of the plurality of modified cell culture substrates, forming a plurality of cultured single plasma cells by culturing the seeded each respective plasma cell in a culture medium for a time duration of at least 10 days, and detecting the one or more single plasma cells that secrete the antibody fragment from among the plurality of cultured single plasma cells. The culture medium comprises a conditioned medium harvested from a culture of h-BMSCs, Insulin, Transferrin, Selenium, and Pyruvate.