摘要:
The biosensor of this invention can quantify a substrate in a sample liquid by electrochemically measuring the amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate and an oxidoreductase. The biosensor has an electrically insulating substrate and an electrode system formed on the substrate including a working electrode, a counter electrode and a third electrode used for detecting a liquid junction. The third electrode can be used merely for detecting a liquid junction, or can be used as both a reference electrode and a liquid junction detecting electrode.
摘要:
The present invention provides a biosensor having a favorable response characteristic up to a high concentration range, a low blank response and a high storage stability. This sensor comprises an electrode system including a working electrode and a counter electrode, for forming an electrochemical measurement system by coming in contact with a supplied solution; an electrically insulating supporting member for supporting the electrode system; a first reagent layer formed on the working electrode; and a second reagent layer formed on the counter electrode, wherein the first reagent layer comprises an enzyme as the main component, and the second reagent layer comprises an electron mediator as the main component.
摘要:
A method using a biosensor is disclosed which facilitates high accuracy quantitation of a substrate by best eliminating adverse effects of red blood cell existing in blood together with the substrate. The biosensor comprises an electrode system including a working electrode and a counter electrode formed on an electrically insulating base plate, and a reaction layer containing at least an oxidoreductase and an electron acceptor, wherein the electron acceptor is a sodium salt.
摘要:
The biosensor of the present invention has a reaction layer that contains at least an oxidoreductase and an electron acceptor and is formed on an electrode system including a working electrode and a counter electrode on a base plate. The working electrode, the counter electrode, and lead conductors connecting with these electrodes are made of carbon. The counter electrode is formed in a substantially C shape, and the working electrode is arranged in a recess of the substantially C-shaped counter electrode. An insulating layer surrounds the counter electrode and the working electrode. A cover member with a groove is combined with the base plate to define a sample supply channel that is formed therebetween and runs toward the electrode system. The groove extends from one end of the base plate and passes over the electrode system, and the insulating layer extends to an air hole formed in a terminal of the groove of the cover member. This arrangement prevents exposure of part of the lead conductor connecting with the working electrode to the sample solution and development of an error in measurement.
摘要:
A biosensor for quantification of a specific component contained in various biological samples comprises an electrically insulating base plate, an electrode system including a working electrode and a counter electrode which are provided on the electrically insulating base plate, a reaction layer formed on the electrode system including at least an oxido-reductase, and an enclosure member having a hollow space constituting a sample supplying channel on the electrically insulating base plate, wherein substantially the whole of the reaction layer is exposed to the hollow space.
摘要:
A highly sensitive biosensor that uses a smaller amount of sample for measurement is described. The biosensor includes a first insulating base plate having a working electrode, a second insulating base plate having a counter electrode opposed to the working electrode, and a reagent layer containing at least an oxidoreductase. The biosensor further includes a sample supply pathway formed between the first and second insulating base plates, where the working electrode, counter electrode and reagent layer are exposed to an inside of the sample supply pathway, and the distance between the working electrode and the counter electrode is 150 μm or less.
摘要:
An apparatus for determining the concentration of a substrate in a sample solution using an electrode system comprising a working electrode, a counter electrode, and a reaction layer which contains at least an oxidoreductase and an electron mediator and is formed on the electrode system to electrochemically measure a reduced amount of the electron mediator resulting from enzyme reaction in the reaction layer, wherein a third electrode is formed as an interfering substance detecting electrode. A current flowing between the counter electrode and the third electrode is measured which is taken as a positive error. Subsequently, voltage application between the counter electrode and the third electrode is released and a voltage for oxidizing the reduced form electron mediator is applied between the working electrode and the counter electrode to measure a current flowing between the two electrodes.
摘要:
The present invention provides a method for quantifying a substrate having improved measurement accuracy and measurement sensitivity. According to the method for quantifying the substrate of the present invention, a constant voltage is applied to an electrode system, between a working electrode and a counter electrode, via a resistor connected in series with the electrode system after placing a sample solution containing the substrate on the electrode system, so as to oxidize an electron acceptor, which has been reduced by an enzyme reaction of the substrate, thereby determining a concentration of the substrate included in the sample solution based on an oxidizing current flowing in the course of oxidation.
摘要:
The biosensor of this invention can quantify a substrate in a sample liquid by electrochemically measuring the amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate and an oxidoreductase. The biosensor has an electrically insulating substrate and an electrode system formed on the substrate including a working electrode, a counter electrode and a third electrode used for detecting a liquid junction. The third electrode can be used merely for detecting a liquid junction, or can be used as both a reference electrode and a liquid junction detecting electrode.
摘要:
An examination system for bodily fluids includes a vessel-like examination unit having an opening, a sensor for examining the bodily fluids, and a bodily-fluids-oozing device. This system is further provided with a pump for decompressing the inside of the unit and an electromagnetic cross-valve for releasing the decompressed condition, or still further provided with humidifying means for supplying vapor inside the unit, in addition to the pump and the valve. As a result, the system produces a precise measurement with a little amount of bodily fluid.