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    Method for quantitative measurement of a substrate
    1.
    发明授权
    Method for quantitative measurement of a substrate 失效
    定量测定基质的方法

    公开(公告)号：US06225078B1

    公开(公告)日：2001-05-01

    申请号：US09122591

    申请日：1998-07-27

    申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

    发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

    IPC分类号： C12Q126

    CPC分类号： C12Q1/004 ,  C12Q1/006

    摘要： The invention provides a method for quantitative measurement of a substrate with high accuracy by electrochemically oxidizing an electron mediator which has been reduced by enzyme reaction thereby determining the substrate concentration based on a current flowing during electrochemical oxidation from which adverse effects of an easy-to-oxidize substance on the oxidation process have been minimized. The quantitating method in accordance with the present invention comprises a first step for causing a substrate contained in a sample to react with a specific oxidoreductase to the substrate in the presence of an electron mediator in oxidized state, and a second step for electrochemically reducing the electron mediator in oxidized state which remains non-reduced by the enzyme reaction in the first step, thereby obtaining a current flowing during electrochemical reduction.

    摘要翻译： 本发明提供了一种用于通过电化学氧化已经通过酶反应降低的电子介体来高精度地定量测量衬底的方法，从而基于在电化学氧化期间流动的电流来确定衬底浓度，从而容易地 - 在氧化过程中氧化物质已被最小化。 根据本发明的定量方法包括第一步骤，用于在电子介体的氧化态存在下使包含在样品中的底物与特定的氧化还原酶与底物反应，第二步用于电化学还原电子 在第一步中通过酶反应保持未还原的氧化态介体，从而获得在电化学还原过程中流动的电流。

    Biosensor and method of manufacturing the same
    2.
    发明授权
    Biosensor and method of manufacturing the same 失效
    生物传感器及其制造方法

    公开(公告)号：US6077408A

    公开(公告)日：2000-06-20

    申请号：US002368

    申请日：1998-01-02

    申请人： Yoshiko Miyamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

    发明人： Yoshiko Miyamoto ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

    IPC分类号： G01N27/327 ,  C12Q1/00 ,  C12Q1/26 ,  G01N27/26

    CPC分类号： C12Q1/001 ,  C12Q1/26

    摘要： Disclosed is a biosensor facilitating high accuracy quantification of a specific component in a sample solution with no adverse influence of solid substances. The biosensor comprises an electrically insulating base plate, an electrode system comprising a working electrode and a counter electrode formed on the base plate, a reaction layer containing at least one enzyme disposed on the electrode system, and an anionic filter formed on the reaction layer for inhibiting permeation of solid components.

    摘要翻译： 公开了一种促进样品溶液中特定组分的高精度定量的生物传感器，其不受固体物质的不利影响。 生物传感器包括电绝缘基板，包括在基板上形成的工作电极和对电极的电极系统，包含设置在电极系统上的至少一个酶的反应层和形成在反应层上的阴离子过滤器 抑制固体组分的渗透。

    Biosensor and method for quantitative measurement of a substrate using the same
    3.
    发明授权
    Biosensor and method for quantitative measurement of a substrate using the same 有权
    生物传感器和使用其的基板的定量测量方法

    公开(公告)号：US5906921A

    公开(公告)日：1999-05-25

    申请号：US159686

    申请日：1998-09-24

    申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

    发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

    IPC分类号： C12Q1/00 ,  G01N33/543 ,  C12Q1/26 ,  C12Q1/54

    CPC分类号： C12Q1/004 ,  G01N33/5438 ,  Y10S435/817

    摘要： A biosensor is disclosed for application to a method for quantitative measurement of a substrate comprising a first step of causing a substrate contained in a sample to react with an oxidoreductase specific to the substrate in the presence of an electron mediator in oxidized state, a second step of applying a potential to a working electrode for reducing the electron mediator in oxidized state that remains not reduced in the first step, and a third step of measuring a reduction current flowing across the working electrode and a counter electrode. The biosensor comprises an electrically insulating base plate, an electrode system having a working electrode and a counter electrode formed on the base plate, and a reaction layer which is formed on or in the vicinity of the electrode system and contains at least an oxidoreductase and an electron mediator, the counter electrode including at least a reductant of a redox compound or an electrolytically oxidizable metal.

    摘要翻译： 公开了用于定量测量基材的方法的生物传感器，其包括第一步骤，使第一步骤使得包含在样品中的底物在氧化态的电子介体存在下与特定于氧化还原酶的氧化还原酶反应，第二步 在第一步骤中向工作电极施加电位以还原氧化态的电子介体，其保持不减少;以及第三步骤，测量流过工作电极和对电极的还原电流。 生物传感器包括电绝缘基板，具有工作电极和形成在基板上的对电极的电极系统和形成在电极系统上或附近的反应层，至少含有氧化还原酶和 电子介体，所述对电极至少包含氧化还原化合物或电解可氧化金属的还原剂。

    Method for quantifying specific compound
    4.
    发明授权
    Method for quantifying specific compound 失效
    定量化合物的方法

    公开(公告)号：US5565085A

    公开(公告)日：1996-10-15

    申请号：US428040

    申请日：1995-04-25

    申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno

    发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno

    IPC分类号： G01N27/416 ,  C12Q1/00 ,  G01N27/30 ,  G01N27/327 ,  G01N27/26

    CPC分类号： C12Q1/001 ,  Y10S435/817

    摘要： A biosensor for quantifying a specific compound has a reaction layer containing at least an enzyme, and an electrode system having a working electrode and a counter electrode formed on an insulating base plate; the biosensor detects the specific compound contained in a sample on the basis of an electrochemical response. In quantifying, the working electrode and the counter electrode are short-circuited before the voltage is applied therebetween. The short-circuiting eliminates measuring errors that may occur due to nonuniform dissolution of the reaction layer in a sample, and achieves highly reliable quantification of a specific compound.

    摘要翻译： 用于定量特定化合物的生物传感器具有至少含有酶的反应层和在绝缘基板上形成有工作电极和对电极的电极系统; 生物传感器基于电化学响应来检测样品中包含的特定化合物。 在量化之前，工作电极和对电极在施加电压之前短路。 短路消除了由于反应层在样品中的不均匀溶解而可能发生的测量误差，并且实现了特定化合物的高度可靠的定量。

    Device and method for determining the concentration of a substrate
    6.
    发明授权
    Device and method for determining the concentration of a substrate 有权
    用于确定底物浓度的装置和方法

    公开(公告)号：US06340428B1

    公开(公告)日：2002-01-22

    申请号：US09424715

    申请日：1999-11-29

    申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

    发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

    IPC分类号： G01N2726

    CPC分类号： C12Q1/004

    摘要： A method for determining the concentration of a substrate in a sample solution using an electrode system comprising a working electrode, a counter electrode, and a reaction layer which contains at least an oxidoreductase and an electron mediator and is formed on the electrode system to electrochemically measure a reduced amount of the electron mediator resulting from enzyme reaction in the reaction layer, wherein a third electrode is formed as an interfering substance detecting electrode. A current flowing between the counter electrode and the third electrode is measured which is taken as a positive error. Subsequently, voltage application between the counter electrode and the third electrode is released and a voltage for oxidizing the reduced form electron mediator is applied between the working electrode and the counter electrode to measure a current flowing between the two electrodes.

    摘要翻译： 一种使用包括工作电极，对电极和至少包含氧化还原酶和电子介体的反应层的电极系统来确定样品溶液中的衬底的浓度的方法，并且形成在电极系统上以电化学测量 在反应层中由酶反应产生的电子介体的量减少，其中形成第三电极作为干扰物质检测电极。 测量在对电极和第三电极之间流动的电流，其被认为是正误差。 随后，释放对电极和第三电极之间的电压施加，并且在工作电极和对电极之间施加用于氧化还原形式的电子介体的电压，以测量在两个电极之间流动的电流。

    Biosensor
    7.
    发明授权
    Biosensor 有权
    生物传感器

    公开(公告)号：US06212417B1

    公开(公告)日：2001-04-03

    申请号：US09379128

    申请日：1999-08-20

    申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

    发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

    IPC分类号： A61B505

    CPC分类号： G01N27/3272

    摘要： A highly reliable biosensor is provided which is reduced in influences of any interfering substance or preexisting oxygen in a sample solution. The biosensor comprises an electrode system including a working electrode, a counter electrode and a third electrode also usable as an interfering substance detecting electrode, a reagent layer containing at least an oxidoreductase and an electron mediator, and an electrically insulating base plate for supporting the electrode system and the reagent layer, wherein the third electrode is arranged at an opposing position to that of at least one of the working electrode and the counter electrode and the reagent layer is arranged at a predetermined position apart from the third electrode.

    摘要翻译： 提供了高度可靠的生物传感器，其减少了样品溶液中任何干扰物质或预先存在的氧气的影响。 生物传感器包括电极系统，其包括工作电极，对电极和也可用作干扰物质检测电极的第三电极，至少含有氧化还原酶和电子介体的试剂层，以及用于支撑电极的电绝缘基板 系统和试剂层，其中第三电极布置在与工作电极和对电极中的至少一个相对的位置，并且试剂层布置在与第三电极隔开的预定位置处。

    Electrode probe and body fluid examination equipment including the same
    8.
    发明授权
    Electrode probe and body fluid examination equipment including the same 有权
    电极探针和体液检查设备包括相同

    公开(公告)号：US6104940A

    公开(公告)日：2000-08-15

    申请号：US326350

    申请日：1999-06-04

    申请人： Motokazu Watanabe ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

    发明人： Motokazu Watanabe ,  Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai

    IPC分类号： G01N27/30 ,  A61B5/00 ,  A61B5/145 ,  A61B5/1486 ,  A61B5/15 ,  A61B5/157 ,  A61B5/05

    CPC分类号： A61B5/1486 ,  A61B5/6848

    摘要： Disclosed is an electrode probe and a body fluid examination equipment enabling examination of a trace amount of body fluid. The electrode probe comprises an insulating needle member, a working electrode lead disposed in the needle member, a working electrode whose lead is protruding outside from the needle member, a counter electrode disposed on the surface of the needle member, a lead connected to the counter electrode, and a reagent layer disposed in contact with or in the vicinity of the working and counter electrodes of the electrode system. When a trace amount of body fluid leaking on the skin surface is brought into contact with the reagent layer, the reagent layer dissolves which results in electron transfer whereby information about the concentration of analyte in the body fluid can be obtained from the electrode system.

    摘要翻译： 公开了能够检测微量体液的电极探针和体液检查装置。 电极探针包括绝缘针构件，设置在针构件中的工作电极引线，其引线从针构件向外突出的工作电极，设置在针构件表面上的对电极，连接到计数器的引线 电极和设置成与电极系统的工作电极和对电极接触或附近的试剂层。 当皮肤表面泄漏的微量体液与试剂层接触时，试剂层溶解，导致电子转移，从而可以从电极系统获得关于体液中分析物浓度的信息。