公开(公告)号：US5582697A

公开(公告)日：1996-12-10

申请号：US425820

申请日：1995-04-20

申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno ,  Syoji Miyazaki

发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno ,  Syoji Miyazaki

IPC分类号： C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/005 ,  C12Q1/001 ,  Y10S435/817

摘要： The biosensor of this invention can quantify a substrate in a sample liquid by electrochemically measuring the amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate and an oxidoreductase. The biosensor has an electrically insulating substrate and an electrode system formed on the substrate including a working electrode, a counter electrode and a third electrode used for detecting a liquid junction. The third electrode can be used merely for detecting a liquid junction, or can be used as both a reference electrode and a liquid junction detecting electrode.

摘要翻译： 本发明的生物传感器可以通过电化学测量由底物和氧化还原酶之间的反应中产生的电子而减少的电子受体的量来量化样品液体中的底物。 生物传感器具有电绝缘基板和形成在基板上的电极系统，该基板包括工作电极，对电极和用于检测液体接合点的第三电极。 第三电极可以仅用于检测液体接点，也可以用作参考电极和液体接点检测电极。

公开(公告)号：US5650062A

公开(公告)日：1997-07-22

申请号：US526557

申请日：1995-09-12

申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno ,  Syoji Miyazaki

发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno ,  Syoji Miyazaki

IPC分类号： C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/001 ,  Y10S435/817

摘要： The biosensor of this invention can quantify a substrate in a sample liquid by electrochemically measuring the amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate and an oxidoreductase. The biosensor has an electrically insulating substrate and an electrode system formed on the substrate including a working electrode, a counter electrode and a third electrode used for detecting a liquid junction. The third electrode can be used merely for detecting a liquid junction, or can be used as both a reference electrode and a liquid junction detecting electrode.

摘要翻译： 本发明的生物传感器可以通过电化学测量由底物和氧化还原酶之间的反应中产生的电子而减少的电子受体的量来量化样品液体中的底物。 生物传感器具有电绝缘基板和形成在基板上的电极系统，该基板包括工作电极，对电极和用于检测液体接合点的第三电极。 第三电极可以仅用于检测液体接点，也可以用作参考电极和液体接点检测电极。

公开(公告)号：US5565085A

公开(公告)日：1996-10-15

申请号：US428040

申请日：1995-04-25

申请人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno

发明人： Shin Ikeda ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno

IPC分类号： G01N27/416 ,  C12Q1/00 ,  G01N27/30 ,  G01N27/327 ,  G01N27/26

CPC分类号： C12Q1/001 ,  Y10S435/817

摘要： A biosensor for quantifying a specific compound has a reaction layer containing at least an enzyme, and an electrode system having a working electrode and a counter electrode formed on an insulating base plate; the biosensor detects the specific compound contained in a sample on the basis of an electrochemical response. In quantifying, the working electrode and the counter electrode are short-circuited before the voltage is applied therebetween. The short-circuiting eliminates measuring errors that may occur due to nonuniform dissolution of the reaction layer in a sample, and achieves highly reliable quantification of a specific compound.

摘要翻译： 用于定量特定化合物的生物传感器具有至少含有酶的反应层和在绝缘基板上形成有工作电极和对电极的电极系统; 生物传感器基于电化学响应来检测样品中包含的特定化合物。 在量化之前，工作电极和对电极在施加电压之前短路。 短路消除了由于反应层在样品中的不均匀溶解而可能发生的测量误差，并且实现了特定化合物的高度可靠的定量。

公开(公告)号：US5512159A

公开(公告)日：1996-04-30

申请号：US293131

申请日：1994-08-19

申请人： Toshihiko Yoshioka ,  Mariko Kawaguri ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno ,  Shoji Miyazaki

发明人： Toshihiko Yoshioka ,  Mariko Kawaguri ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno ,  Shoji Miyazaki

IPC分类号： G01N27/28 ,  C12M1/34 ,  C12M1/40 ,  C12Q1/00 ,  C12Q1/54 ,  G01N27/327 ,  G01N33/483

CPC分类号： C12Q1/001

摘要： A biosensor of the present invention is provided with an insulating base plate, an electrode system mainly consisting of a working electrode and a counter electrode formed on the insulating base plate, and a reaction layer on the electrode system. The counter electrode is partially a circular arc. A manufacturing method of the biosensor of the present invention is comprised of a step to form a base by arranging leads, an electrode system, and an insulating layer on an insulating base plate, and a step to form a reaction layer mainly composed of an enzyme on the electrode system. Since the counter electrode is partially in the shape of a circular arc, the reaction layer is easily formed and prevented from delaminating, whereby the measuring accuracy, preservative properties and reliability of the biosensor are improved. The present invention enables highly efficient biosensors to be mass produced at low cost.

摘要翻译： 本发明的生物传感器设置有绝缘基板，主要由在绝缘基板上形成的工作电极和对电极组成的电极系统和电极系统上的反应层。 对电极部分是圆弧。 本发明的生物传感器的制造方法包括通过在绝缘基板上排列引线，电极系统和绝缘层形成基底的步骤，以及形成主要由酶组成的反应层的步骤 在电极系统上。 由于对电极部分为圆弧形状，反应层易于形成并防止分层，从而提高了生物传感器的测量精度，防腐性能和可靠性。 本发明能够以低成本批量生产高效生物传感器。

公开(公告)号：US06773564B1

公开(公告)日：2004-08-10

申请号：US09406832

申请日：1999-09-28

申请人： Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata ,  Shoji Miyazaki ,  Hideyuki Baba ,  Seiji Takeshima

发明人： Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata ,  Shoji Miyazaki ,  Hideyuki Baba ,  Seiji Takeshima

IPC分类号： G01N27327

CPC分类号： C12Q1/006

摘要： The present invention provides a high performance glucose sensor which can demonstrate high stability against preservation and produces only a low blank value. The glucose sensor comprises an electrically insulating base plate, an electrode system formed on the base plate, and a reaction layer which is formed in contact with or in the vicinity of the electrode system and contains at least a glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, the reaction layer further containing an additive such as phthalic acid.

摘要翻译： 本发明提供了一种高性能葡萄糖传感器，其可以显示对保存的高稳定性并且仅产生低的空白值。 葡萄糖传感器包括电绝缘基板，形成在基板上的电极系统和与电极系统接触或附近形成的反应层，并且至少含有辅酶是吡咯并喹啉的葡萄糖脱氢酶 醌，反应层还含有邻苯二甲酸等添加剂。

公开(公告)号：US07005048B1

公开(公告)日：2006-02-28

申请号：US09807692

申请日：2000-10-02

申请人： Motokazu Watanabe ,  Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Nakayama ,  Shoji Miyazaki ,  Hideyuki Baba

发明人： Motokazu Watanabe ,  Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Nakayama ,  Shoji Miyazaki ,  Hideyuki Baba

IPC分类号： G01N27/327

CPC分类号： C12Q1/006

摘要： The present invention provides a high-performance glucose sensor having excellent storage stability and an improved response characteristic. This sensor comprises: an electrically insulating base plate; an electrode system including at least a working electrode and a counter electrode formed on the base plate; and a reaction layer containing at least pyrrolo-quinoline quinone dependent glucose dehydrogenase, formed in contact with or in the vicinity of the electrode system, and the reaction layer contains at least one kind of additive selected from the group consisting of gluconic acid and salts thereof.

摘要翻译： 本发明提供一种具有优异的储存稳定性和改善的响应特性的高性能葡萄糖传感器。 该传感器包括：电绝缘基板; 电极系统，其至少包括形成在所述基板上的工作电极和对电极; 和至少含有与电极体系接触或附近形成的吡咯并喹啉醌依赖型葡萄糖脱氢酶的反应层，反应层含有选自葡萄糖酸及其盐的至少一种添加剂 。

公开(公告)号：US07122698B2

公开(公告)日：2006-10-17

申请号：US10680919

申请日：2003-10-08

申请人： Hiroshi Yoshida ,  Hideaki Tsuneki ,  Toshio Hayashi ,  Hideyuki Baba ,  Takahiro Inagaki ,  Satoshi Nakagawa ,  Yukihiko Kakimoto ,  Ritsuo Kitada ,  Kohei Umehara

发明人： Hiroshi Yoshida ,  Hideaki Tsuneki ,  Toshio Hayashi ,  Hideyuki Baba ,  Takahiro Inagaki ,  Satoshi Nakagawa ,  Yukihiko Kakimoto ,  Ritsuo Kitada ,  Kohei Umehara

IPC分类号： C07C69/66

CPC分类号： C07C67/40 ,  C07C69/675

摘要： The present invention provides a process for more efficiently producing an α-hydroxycarboxylic acid ester wherein side reactions due to the α-hydroxycarboxylic acid ester are inhibited or prevented in comparison with prior art production processes.The invention provides a process for producing an α-hydroxycarboxylic acid ester comprising Steps 1 to 3:Step 1. reacting, in the presence of oxygen, (i) a 1,2-diol with a 1,2-diol or (ii) a 1,2-diol with an alcohol to obtain a reaction product containing an α-hydroxycarboxylic acid ester;Step 2. separating the α-hydroxycarboxylic acid ester from the reaction product obtained in Step 1 by distillation under reduced pressure; andStep 3. feeding Step 1 with a mixture obtained by partially or entirely removing water from the reaction product, wherein the mixture contains an unreacted 1,2-diol and/or alcohol.

摘要翻译： 本发明提供了一种更有效地制备α-羟基羧酸酯的方法，其中与现有技术的制备方法相比，由于α-羟基羧酸酯的副反应被抑制或被阻止。 本发明提供了一种制备α-羟基羧酸酯的方法，其包括步骤1至3：步骤1，在氧的存在下，（i）1,2-二醇与1,2-二醇或（ii） 具有醇的1,2-二醇以获得含有α-羟基羧酸酯的反应产物; 步骤2.通过减压蒸馏从步骤1中获得的反应产物中分离α-羟基羧酸酯; 和步骤3.向通过部分或全部从反应产物中除去水而获得的混合物进料步骤1，其中混合物含有未反应的1,2-二醇和/或醇。

公开(公告)号：US20060079711A1

公开(公告)日：2006-04-13

申请号：US11290174

申请日：2005-11-30

申请人： Toshio Hayashi ,  Hideyuki Baba

发明人： Toshio Hayashi ,  Hideyuki Baba

IPC分类号： C07C69/66

CPC分类号： C08G63/06 ,  C07C51/00 ,  C07C51/09 ,  C07C67/40 ,  Y02P20/582 ,  C07C59/06 ,  C07C69/675

摘要： A method of producing a diol derivative efficiently and to high purity is provided. Specifically, the present invention relates to a method of producing a diol derivative having, as a fundamental step, a step of obtaining an α-hydroxycarboxylic acid ester by reacting (i) one or more 1,2-diols or (ii) a 1,2-diol and a primary alcohol as starting material(s) with oxygen in the presence of a catalyst comprising metal loaded on a carrier, further hydrolyzing the α-hydroxycarboxylic acid ester to obtain α-hydroxycarboxylic acid, and subjecting the α-hydroxycarboxylic acid to polycondensation.

摘要翻译： 提供了有效且高纯度地生产二醇衍生物的方法。 具体地说，本发明涉及一种制备二醇衍生物的方法，其作为基本步骤，通过使（ⅰ）一种或多种1,2-二醇或（ⅱ）ⅰ-羟基羧酸酯反应获得α-羟基羧酸酯的步骤 ，二醇和伯醇作为起始原料，在含有负载在载体上的金属的催化剂存在下，用氧进一步水解α-羟基羧酸酯以获得α-羟基羧酸，并使α-羟基羧酸 酸缩聚。

公开(公告)号：US6037489A

公开(公告)日：2000-03-14

申请号：US51302

申请日：1998-04-10

申请人： Hideyuki Baba ,  Satoru Miura ,  Masanori Nonoguchi ,  Noboru Saito

发明人： Hideyuki Baba ,  Satoru Miura ,  Masanori Nonoguchi ,  Noboru Saito

IPC分类号： C07C67/54 ,  C07C69/67 ,  C07C69/66 ,  C07C51/46

CPC分类号： C07C67/54

摘要： A method for purifying glyoxylates includes (1) a coarse distillation process in which a crude glyoxylate in which water coexists is, in a film form, continuously subjected to coarse distillation, and (2) an azeotropic dehydration process in which the crude glyoxylate purified through the coarse distillation process is subjected to azeotropic dehydration in the presence of an azeotropic agent such as propyl acetate. By this method, high-purity glyoxylates can be efficiently and easily obtained at lower costs.

摘要翻译： PCT No.PCT / JP97 / 03043 Sec。 371日期：1998年4月10日 102（e）1998年4月10日PCT PCT 1997年8月9日PCT公布。 出版物WO98 / 09936 1998年3月12日，乙醛酸酯的纯化方法包括（1）粗水蒸馏法，其中水共存的粗乙醛酸粗品是薄膜形式，连续进行粗蒸馏，和（2）共沸脱水方法，其中 通过粗蒸馏过程纯化的粗乙醛酸酯在共沸剂如乙酸丙酯存在下进行共沸脱水。 通过该方法，可以以更低的成本有效且容易地获得高纯度的乙醛酸酯。

公开(公告)号：US07122111B2

公开(公告)日：2006-10-17

申请号：US10878536

申请日：2004-06-29

申请人： Hiroyuki Tokunaga ,  Shoji Miyazaki ,  Hideyuki Baba ,  Yoichi Inoue ,  Kazuo Iketaki ,  Katsumi Hamamoto

发明人： Hiroyuki Tokunaga ,  Shoji Miyazaki ,  Hideyuki Baba ,  Yoichi Inoue ,  Kazuo Iketaki ,  Katsumi Hamamoto

IPC分类号： G01N27/327 ,  G01N33/48 ,  G01N31/00

CPC分类号： G01N27/3274 ,  Y10T436/11

摘要： A glucose sensor system comprising the steps of using as a sample discriminating parameter a ratio (I/ΔI) of a measured current value I to the time-differential value of the current value ΔI, defining a discrimination function that discriminates whether a sample is blood or control fluid and uses the discriminating parameter as an independent variable, quantitating as a discriminating index a numeric value obtained by substituting a discriminating parameter value into this discrimination function, and automatically discriminating, based on this index, whether the sample is blood or a control fluid, whereby a kind of the sample can be automatically quantitated by measuring electric current when a sensor system is used for quantitating the concentration of an analysis object in the sample.

摘要翻译： 一种葡萄糖传感器系统，包括以下步骤：将测量电流值I与当前值ΔI的时间微分值的比值（I / DeltaI）用作样本鉴别参数，所述差分值定义了鉴别样品是否为血液的鉴别功能 或控制流体，并将鉴别参数用作独立变量，作为鉴别指标，将通过将鉴别参数值代入该鉴别函数而获得的数值，并根据该指标自动鉴别样品是血液还是控制 流体，从而当使用传感器系统来定量样品中的分析对象的浓度时，可以通过测量电流来自动量化样品的种类。