公开(公告)号：US20100076735A1

公开(公告)日：2010-03-25

申请号：US12533079

申请日：2009-07-31

申请人： Frank HAESELER ,  Francoise BEHAR

发明人： Frank HAESELER ,  Francoise BEHAR

IPC分类号： G06F17/10

CPC分类号： C12Q1/64

摘要： Method of determining the compositional evolution of fluids present in a porous medium as a result of biodegradation.A biodegradation compositional scheme is defined from twelve chemical classes allowing the hydrocarbons and the gases resulting from biodegradation to be described. The initial amount of each one of these chemical classes contained in the fluids before biodegradation is determined. A reaction scheme is then defined for biodegradation, wherein the chemical classes react with electron acceptors according to parallel reactions having different velocities, and each class reacts with these acceptors according to sequential reactions. The biodegradation reaction velocities are determined for each class. The composition of the fluids is then deduced by assessing the amount of each chemical class by applying the reaction scheme. The composition of the biodegraded hydrocarbons, as well as the amount of acid gas and of methane produced, are notably deduced.Application: notably petroleum reservoir development.

摘要翻译： 确定作为生物降解结果存在于多孔介质中的流体的组成演变的方法。 生物降解组成方案由12种化学类型定义，允许描述由生物降解产生的烃和气体。 确定在生物降解之前在流体中包含的这些化学类别中的每一种的初始量。 然后定义反应方案用于生物降解，其中化学类别根据具有不同速度的平行反应与电子受体反应，并且每一类根据顺序反应与这些受体反应。 确定每个等级的生物降解反应速度。 然后通过应用反应方案评估每种化学类的量来推断流体的组成。 生物降解碳氢化合物的组成以及酸性气体和生成的甲烷的量都得到了显着的推广。 应用：特别是石油储层开发。

公开(公告)号：US20070007203A1

公开(公告)日：2007-01-11

申请号：US11478616

申请日：2006-07-03

申请人： Bernard Carpentier

发明人： Bernard Carpentier

IPC分类号： C02F3/00

CPC分类号： C12Q1/64

摘要： Method of evaluating the biodegradation, through the action of a bacterial population, of hydrocarbons trapped in a geologic structure. The mass of hydrocarbons present in said structure is determined without taking account of the biodegradation, from data relative to the physical and geometrical characteristics of the structure studied. Then the mass of hydrocarbons consumed by biodegradation is calculated after evaluating the number of bacteria, their hydrocarbon consumption and the structure filling time. The mass percentage of oil disappeared through the bacterial action is deduced from these two masses. Application: determination of the composition of oils in a reservoir and notably location of heavy oils. The method can notably be applied to the evaluation of the economic risk linked with the presence of biodegraded petroleum reservoirs.

摘要翻译： 通过细菌群体的作用评估被捕获在地质结构中的烃的生物降解的方法。 确定所述结构中存在的烃的质量，而不考虑生物降解，相对于所研究结构的物理和几何特征的数据。 然后通过评估细菌数量，其碳氢化合物消耗量和结构填充时间来计算生物降解消耗的碳氢化合物的质量。 通过细菌作用消失的油的质量百分比是从这两个质量推断的。 应用：测定储层中油的重要组成以及重油的重要位置。 该方法可以显着地应用于与生物降解石油储层存在相关的经济风险评估。

公开(公告)号：US20060154292A1

公开(公告)日：2006-07-13

申请号：US11350796

申请日：2006-02-10

申请人： Akihiko Maruyama ,  Takanori Higashihara ,  Hiroyuki Ishiwata ,  Tsunemi Fujita

发明人： Akihiko Maruyama ,  Takanori Higashihara ,  Hiroyuki Ishiwata ,  Tsunemi Fujita

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/689 ,  C12N15/11 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/64

摘要： An isolated 16S rDNA having a nucleotide sequence selected from SEQ ID NOs: 1, 2, 3, and 4. The invention also relates to an RNA or DNA probe with a length ranging from 10 to 50 bases which comprises at least one nucleotide sequence selected from SEQ ID NOs: 1, 2, 3, and 4; and wherein the probe is hybridizable specifically with a petroleum-degrading bacterium belonging to the genus Cycloclasticus. The invention also relates to isolated 16S rDNA useful for detecting and screening for petroleum-degrading bacteria.

摘要翻译： 具有选自SEQ ID NO：1,2,3和4的核苷酸序列的分离的16S rDNA。本发明还涉及长度为10至50个碱基的RNA或DNA探针，其包含至少一个选自的核苷酸序列 来自SEQ ID NO：1,2,3和4; 并且其中所述探针与属于旋毛虫属的石油降解细菌特异性杂交。 本发明还涉及用于检测和筛选石油降解细菌的分离的16S rDNA。

公开(公告)号：US5055397A

公开(公告)日：1991-10-08

申请号：US134431

申请日：1987-12-17

申请人： Glenda B. Michaels ,  Walter C. Riese

发明人： Glenda B. Michaels ,  Walter C. Riese

IPC分类号： C12Q1/64

CPC分类号： C12Q1/64

摘要： Geomicrobiological exploration method employing one or more culture, luminometry or tritiated thymidine assays of microbes (bacteria) for sensitivity, determined as survival rate in percentage, of the microbes to selected toxic materials, preferably heavy metals and/or hydrocarbons. The survival assay values are plotted as contours on a geophysical map and target areas of potential interest are identified and further evaluated by inspection or other techniques. Examples show actual use of the techniques to identify a target area in a raw prospect petroleum lease area which was drilled to successful oil discovery thus proving the method. Sensitivity test incubation ranges from 1-3 hours (preferably 2 hours) for heavy metals at 20-25 degrees C., and 3-10 min. for pentene/hexane at 15-20 degrees C. Luminometry is fastest, being run in 1.5-30 minutes and most suitable for field surveys. Toxics concentrations may range from 0.001 ug/ml to 15,000 ug/ml for heavy metals to 0.001-25 vol % for hydrocarbons.

摘要翻译： 使用微生物（微生物）的微生物（细菌）的敏感性的一种或多种培养，发光测定或氚标记的胸苷测定的微生物学探索方法，其被确定为选择的有毒物质，优选重金属和/或碳氢化合物的微生物的存活率。 将生存测定值绘制为地球物理图上的轮廓，并鉴定潜在感兴趣的目标区域，并通过检查或其他技术进一步评估。 实例表明，这些技术的实际用途是确定原始前景石油租赁区域中的目标区域，该区域是为成功的石油开采而钻探的，从而证明了该方法。 敏感性试验温度范围为20-25摄氏度的重金属1-3小时（优选2小时），3-10分钟。 对于戊烯/己烷在15-20摄氏度。光度法最快，在1.5-30分钟内运行，最适合实地调查。 对于重金属，毒素浓度可以为0.001ug / ml至15,000ug / ml，对于碳氢化合物为0.001-25vol％。

公开(公告)号：US3985619A

公开(公告)日：1976-10-12

申请号：US490153

申请日：1974-07-19

申请人： Anthony Rene Barringer

发明人： Anthony Rene Barringer

IPC分类号： C12M1/26 ,  C12K1/04 ,  C12K1/06

CPC分类号： C12M41/36 ,  C12Q1/64 ,  G01V9/007 ,  G01N1/2205

摘要： Exploration method and apparatus in which atmospheric particulates are collected, and wherein micro-organisms present in the collected particulates are analyzed for pre-determined elements or compounds.