Method of detecting and quantitating microorganism having specific function and its gene from natural environment using novel 16SrRNA gene data or probes
    1.
    发明申请
    Method of detecting and quantitating microorganism having specific function and its gene from natural environment using novel 16SrRNA gene data or probes 审中-公开
    使用新型16SrRNA基因数据或探针,从天然环境中检测和定量具有特定功能的微生物及其基因的方法

    公开(公告)号:US20060147976A1

    公开(公告)日:2006-07-06

    申请号:US11350955

    申请日:2006-02-10

    IPC分类号: C12Q1/68 C07H21/04

    摘要: A method of detecting and quantitating a petroleum-digesting microorganism and its gene from natural environment. In one embodiment, the microorganism is a bacterium belonging to the genus Cycloclasticus. The invention also relates to a method for detecting and quantitating a petroleum-degrading bacterium belonging to the genus Cycloclasticus comprising using an RNA or DNA probe having a length of from 10 to 50 bases with a part of the nucleotide sequence of the probe represented by any of SEQ ID NOS: 1 to 4; and wherein the probe is hybridizable specifically with a petroleum-degrading bacterium belonging to the genus Cycloclasticus.

    摘要翻译: 一种从天然环境中检测和定量石油消化微生物及其基因的方法。 在一个实施方式中,所述微生物是属于环状克氏杆菌属的细菌。 本发明还涉及一种用于检测和定量属于旋毛虫属的降解石油的细菌的方法,其包括使用长度为10至50个碱基的RNA或DNA探针,其中部分探针的核苷酸序列由任何 SEQ ID NO:1至4; 并且其中所述探针与属于旋毛虫属的石油降解细菌特异性杂交。

    Chitinase and method for preparing the same
    4.
    发明授权
    Chitinase and method for preparing the same 失效
    几丁质酶及其制备方法

    公开(公告)号:US06352850B1

    公开(公告)日:2002-03-05

    申请号:US09661808

    申请日:2000-09-14

    IPC分类号: C12N924

    CPC分类号: C12Y302/01014 C12N9/2442

    摘要: Chitinase with the following physico-chemical properties 1 to 6: 1. action: random cutting of the &bgr;-1,4 bond of chitin to generate the tetramer and dimer of Nacetylglucosamine; 2. optimum pH: 6.5 to 10.4; 3. stable pH: 7.0 to 9.0; 4. optimum temperature: 37° C.; 5. active temperature range: 4 to 60° C.; and 6. thermal stability: 60% or more of the initial activity as retained even after heating at 40° C. and pH 8.0 for 30 minutes; and a method for producing chitinase, comprising culturing a chitinase-generating bacterium reacting with chitin to generate the N-acetylglucosamine oligomer and collecting chitinase from the culture.

    摘要翻译: 具有以下物理化学性质的几丁质酶1至6:1。 作用:随机切割几丁质的β-1,4键以产生N-乙酰基葡萄糖胺的四聚体和二聚体; 2。 最适pH:6.5〜10.4; 3。 稳定pH:7.0〜9.0; 4。 最佳温度:37℃; 5。 有效温度范围:4〜60℃。 和6。 热稳定性:即使在40℃,pH8.0加热30分钟后,初始活性也保持60%以上。 以及生产几丁质酶的方法,包括培养与几丁质反应的几丁质酶生成细菌以产生N-乙酰葡糖胺低聚物并从培养物中收集几丁质酶。

    Clocking device
    9.
    发明授权
    Clocking device 有权
    钟表

    公开(公告)号:US06567345B1

    公开(公告)日:2003-05-20

    申请号:US09554234

    申请日:2000-06-20

    IPC分类号: G04B1904

    摘要: A timepiece has an ordinary time measuring section including an ordinary time display unit for measuring an ordinary time and time information (i.e., chronograph) measuring sections including chronograph displays for measuring time information other than the ordinary time (i.e., chronograph information). The timepiece is configured such that the ordinary time display unit and the chronograph display units, are disposed on a display surface of the timepiece without overlapping each other.

    摘要翻译: 钟表具有通常的时间测量部分,其包括普通时间显示单元,用于测量普通时间和时间信息(即,计时码表)测量部分,该测量部分包括用于测量除了普通时间之外的时间信息的计时显示器(即计时码表信息)。 钟表被构造成使得普通时间显示单元和计时器显示单元被布置在钟表的显示表面上而不重叠。

    Time measurement device
    10.
    发明授权
    Time measurement device 有权
    时间测量装置

    公开(公告)号:US06466518B1

    公开(公告)日:2002-10-15

    申请号:US09446376

    申请日:2000-02-24

    IPC分类号: G04B900

    摘要: A time measurement device includes a first motor (1300) for indicating standard time, a second motor (1400) for indicating a chronograph, a generator (1600) which generates driving power for driving the first and second motors by converting mechanical energy into electrical energy, and a zero reset mechanism (1200) for mechanically resetting the chronograph to zero. A compact time measurement device, operable from a low power, is thus provided.

    摘要翻译: 时间测量装置包括用于指示标准时间的第一马达(1300),用于指示计时器的第二马达(1400),通过将机械能转换为电能来产生用于驱动第一和第二马达的驱动力的发电机(1600) ,以及用于将计时器机械地复位到零的零复位机构(1200)。 因此,提供了可从低功率操作的紧凑的时间测量装置。