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    Chitinase and method for preparing the same
    2.
    发明授权
    Chitinase and method for preparing the same 失效
    几丁质酶及其制备方法

    公开(公告)号：US06352850B1

    公开(公告)日：2002-03-05

    申请号：US09661808

    申请日：2000-09-14

    Applicant: Shizu Fujishima ,  Naoko Yamano ,  Akihiko Maruyama ,  Takanori Higashihara

    Inventor： Shizu Fujishima ,  Naoko Yamano ,  Akihiko Maruyama ,  Takanori Higashihara

    IPC: C12N924

    CPC classification number: C12Y302/01014 ,  C12N9/2442

    Abstract: Chitinase with the following physico-chemical properties 1 to 6: 1. action: random cutting of the &bgr;-1,4 bond of chitin to generate the tetramer and dimer of Nacetylglucosamine; 2. optimum pH: 6.5 to 10.4; 3. stable pH: 7.0 to 9.0; 4. optimum temperature: 37° C.; 5. active temperature range: 4 to 60° C.; and 6. thermal stability: 60% or more of the initial activity as retained even after heating at 40° C. and pH 8.0 for 30 minutes; and a method for producing chitinase, comprising culturing a chitinase-generating bacterium reacting with chitin to generate the N-acetylglucosamine oligomer and collecting chitinase from the culture.

    Abstract translation: 具有以下物理化学性质的几丁质酶1至6：1。 作用：随机切割几丁质的β-1,4键以产生N-乙酰基葡萄糖胺的四聚体和二聚体; 2。 最适pH：6.5〜10.4; 3。 稳定pH：7.0〜9.0; 4。 最佳温度：37℃; 5。 有效温度范围：4〜60℃。 和6。 热稳定性：即使在40℃，pH8.0加热30分钟后，初始活性也保持60％以上。 以及生产几丁质酶的方法，包括培养与几丁质反应的几丁质酶生成细菌以产生N-乙酰葡糖胺低聚物并从培养物中收集几丁质酶。

    Method of detecting and quantitating microorganism having specific function and its gene from natural environment using novel 16SrRNA gene data or probes
    3.
    发明申请
    Method of detecting and quantitating microorganism having specific function and its gene from natural environment using novel 16SrRNA gene data or probes 审中-公开
    使用新型16SrRNA基因数据或探针，从天然环境中检测和定量具有特定功能的微生物及其基因的方法

    公开(公告)号：US20060147976A1

    公开(公告)日：2006-07-06

    申请号：US11350955

    申请日：2006-02-10

    Applicant: Akihiko Maruyama ,  Takanori Higashihara ,  Hiroyuki Ishiwata ,  Tsunemi Fujita

    Inventor： Akihiko Maruyama ,  Takanori Higashihara ,  Hiroyuki Ishiwata ,  Tsunemi Fujita

    IPC: C12Q1/68 ,  C07H21/04

    CPC classification number: C12Q1/689 ,  C12N15/11 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/64

    Abstract: A method of detecting and quantitating a petroleum-digesting microorganism and its gene from natural environment. In one embodiment, the microorganism is a bacterium belonging to the genus Cycloclasticus. The invention also relates to a method for detecting and quantitating a petroleum-degrading bacterium belonging to the genus Cycloclasticus comprising using an RNA or DNA probe having a length of from 10 to 50 bases with a part of the nucleotide sequence of the probe represented by any of SEQ ID NOS: 1 to 4; and wherein the probe is hybridizable specifically with a petroleum-degrading bacterium belonging to the genus Cycloclasticus.

    Abstract translation: 一种从天然环境中检测和定量石油消化微生物及其基因的方法。 在一个实施方式中，所述微生物是属于环状克氏杆菌属的细菌。 本发明还涉及一种用于检测和定量属于旋毛虫属的降解石油的细菌的方法，其包括使用长度为10至50个碱基的RNA或DNA探针，其中部分探针的核苷酸序列由任何 SEQ ID NO：1至4; 并且其中所述探针与属于旋毛虫属的石油降解细菌特异性杂交。

    Microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fatty acids by using the microorganisms
    5.
    发明授权
    Microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fatty acids by using the microorganisms 失效
    能够生产高度不饱和脂肪酸的微生物和通过使用微生物生产高度不饱和脂肪酸的方法

    公开(公告)号：US06582941B1

    公开(公告)日：2003-06-24

    申请号：US08945226

    申请日：1997-12-29

    Applicant: Toshihiro Yokochi ,  Toro Nakahara ,  Takanori Higashihara ,  Satohiro Tanaka ,  Toshiaki Yaguchi

    Inventor： Toshihiro Yokochi ,  Toro Nakahara ,  Takanori Higashihara ,  Satohiro Tanaka ,  Toshiaki Yaguchi

    IPC: C12N114

    CPC classification number: C12P7/6427 ,  A23K20/158 ,  A23K50/75 ,  A23K50/80 ,  A23L15/00 ,  A23L33/12 ,  C11B1/00 ,  C11C3/00 ,  C12P7/6472 ,  C12R1/645 ,  Y10S435/911 ,  Y10S435/946

    Abstract: The present invention relates to the Schizochytrium genus SR21 strain and a microorganism belonging to the same species as does said SR21 strain or having substantially the same fungological properties as does said SR21 strain, the said SR21 strain and microorganism having the ability to produce the (n-3) series of docosahexaenoic acid (DHA) and the (n-6) series of docosapentaenoic acid (DPA), and the invention also relates to a process for preparing the (n-3) series of DHA and the (n-6) series of DPA utilizing said microorganisms. The microorganisms according to the present invention are superior in their proliferation character and their propensity to produce fat, and have the ability to produce the (n-3) series of DHA and the (n-6) series of DPA very well. Accordingly, it is possible to effectively produce the (n-3) series of DHA and/or the (n-6) series of DPA, which are useful in the fields of foods and pharmaceuticals, using the microorganisms according to the present invention. In addition, the present invention provides a fat obtained by culturing the present microorganisms. Since the fat composition contains the (n-6) series of DPA in addition to the (n-3) series of DHA having various physiological activities, it is possible to stably and effectively supply the (n-6) series of DPA and/or the (n-3) series of DHA to subjects in need of these highly unsaturated fatty acids by adding the fat composition to various feedstuffs or foods.

    Abstract translation: 本发明涉及裂殖酵菌属SR21菌株和属于与所述SR21菌株相同种类的微生物或具有与所述SR21菌株基本上相同的真菌性质的微生物，所述SR21菌株和微生物具有产生（n -3）系列二十二碳六烯酸（DHA）和（n-6）系列二十二碳五烯酸（DPA），本发明还涉及一种制备（n-3）系列DHA和（n-6） ）系列的DPA。 根据本发明的微生物的增殖特性及其产生脂肪的倾向是优越的，并且能够非常好地生产（n-3）系列的DHA和（n-6）系列的DPA。 因此，可以使用本发明的微生物有效地生产可用于食品和药物领域的（n-3）系列的DHA和/或（n-6）系列DPA。 此外，本发明提供通过培养本发明的微生物而获得的脂肪。 由于脂肪组合物除了具有各种生理活性的（n-3）系列的DHA以外还含有（n-6）系DPA，所以可以稳定有效地供给（n-6）系列的DPA和/ 或（n-3）系列DHA通过将脂肪组合物添加到各种饲料或食物中来对需要这些高度不饱和脂肪酸的受试者施用。

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