摘要:
An oil and gas exploration method based on a microbial gene is provided, where samples are collected from shallow surface layers above a known oil well, a gas well, and a dry well in an exploration area, DNA is extracted and subjected to high-throughput sequencing (HTS), and a pattern map of a microbial community composition in the exploration area is established according to sequencing results; and characteristic microorganisms in surface soil above an oil/gas well in the exploration area are screened out according to the pattern map, then primers are designed according to attribute characters of the characteristic microorganisms, and samples throughout the exploration area are subjected to fluorescence quantitative polymerase chain reaction (PCR) to detect a number of the characteristic microorganisms.
摘要:
Methods, computer-readable media, and systems for determining oil viscosity based on biodegradation are described. A chemical signature of oil obtained from a subterranean zone is received. An amount of bacteria associated with oil in the zone based on the chemical signature of the oil is received. Using an empirical relationship relating oil viscosities to amounts of bacteria, an oil viscosity that is empirically related to the amount of bacteria is identified. The identified oil viscosity for the oil is provided, for example, in response to input requesting oil viscosity.
摘要:
A method of identifying hydrocarbon seeps that are connected to hydrocarbon reservoirs and for identifying in situ conditions of hydrocarbon reservoirs is disclosed. The method comprises, obtaining a sample from an area of interest, such as a sediment sample or water column sample near a hydrocarbon seep; analyzing the sample to detect microbial signatures that are specific to families associated with hydrocarbon reservoirs; and using the signature to determine whether the hydrocarbon seep is connected to a hydrocarbon reservoir and to identify properties of the hydrocarbon reservoir.
摘要:
A method for onsite bacteria testing for oil and gas applications including collecting at least one component of a wellbore fluid; exposing at least one contaminant in the at least one component to at least one substrate that produces a detectable moiety; and performing a quantitative or qualitative detection of the detectable moiety.
摘要:
The present invention refers to an aerial-supported procedure for the exploration of hydrocarbon deposits, especially crude oil and gas deposits, using fluorescence procedures, including aerial-supported geo-referencing of the detected locations by means of a digital terrain model, employing biosensors which are sensitive to hydrocarbons.
摘要:
Method of modeling the biodegradation of hydrocarbons trapped in an oil reservoir or trap through the action of the bacterial population in an underlying aquifer.From data relative to the reservoir studied, concerning the form and the height of the reservoir, the physical characteristics of the porous medium, the thickness of the transition zone between the hydrocarbons and the water, the composition of the hydrocarbons, of the flow of electron acceptors entering the reservoir and data relative to the bacterial population in the aquifer, modeling is performed by discretizing the reservoir by means of a grid wherein the height of each grid cell is the thickness of the transition zone, and the variation, over the height of the reservoir, of the proportion in heavy fractions of hydrocarbons under the effect of the biodegradation is determined by iterative adjustment in each grid cell of the bacterial population to the amount of hydrocarbons available, the pore space available, the amount of electron acceptors present in the reservoir and the degradation capacities of said population.Applications: determination of the composition of oils in a reservoir and notably of the location of the heaviest fractions.
摘要:
Methods are provided for detection of souring or the likelihood of souring in an oil and gas process fluid which involves analysis of an aqueous sample obtained from an oil and gas process fluid for the presence of bacteria of at least two different bacterial genera, wherein the genera are selected from Pelobacter, Flexistipes, Marinobacterium, Geoalkalibacter, and Halanaerobium. The presence of bacteria from two or more said genera indicates the likelihood of increased sulfide production in the fluid. Other related methods and reagent compositions for performing the methods are provided.
摘要:
A computer supported method, computer system and computer program product for exploring and producing a subsurface heterogeneous hydrocarbon source and generating a predictive production map of an area under investigation, based on microbial prospecting. Microbial data representative of microbial activity attributable to hydrocarbon microseepage and hydrocarbon productivity data of a plurality of geographic locations are retrieved and correlated by the computer in a data correlation algorithm, providing a threshold hydrocarbon productivity quantity and a truncated set of the retrieved microbial data. The set identifies individual types of the microorganisms and corresponding individual weighting factors computed by the data correlation algorithm, for distinguishing prospective hydrocarbon productivity quantities from non-prospective hydrocarbon productivity quantities. An estimated subsurface hydrocarbon productivity quantity for a geographical location of the area under investigation is provided by count weighting retrieved microbial data of this location for microorganisms identified in the truncated set, applying the individual weighting factors.
摘要:
Determining the microorganism load of a substance may be conducted readily using one or more integrated computational elements. By determining a substance's microorganism load, the substance's suitability for a variety of applications may be ascertained. Methods for determining the microorganism load of a substance using one or more integrated computational elements can comprise: providing a substance comprising a plurality of viable microorganisms; exposing the substance to a pulsed light source for a sufficient length of time to form at least some non-viable microorganisms; and determining a microorganism load of the substance using one or more integrated computational elements.
摘要:
Computer-implemented method of determining a composition of hydrocarbons present in a sedimentary basin as a result of biodegradation.The displacement rates of the hydrocarbons and the displacement rates of the water present in the porous medium are estimated, from a genesis time in a mother rock to a possible accumulation in a reservoir rock. The biodegradation in the entire porous medium is then modelled as a function of time, from the genesis in the mother rock, considering that the hydrocarbon composition varies proportionally to the displacement rates.