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公开(公告)号:US08633353B2
公开(公告)日:2014-01-21
申请号:US11981813
申请日:2007-10-30
Applicant: Oliver J. Ratcliffe , Jacqueline Heard , Roderick W. Kumimoto , Peter P. Repetti , T. Lynne Reuber , Robert Creelman , Frederick D. Hempel , Neal L. Gutterson , Roger Canales
Inventor: Oliver J. Ratcliffe , Jacqueline Heard , Roderick W. Kumimoto , Peter P. Repetti , T. Lynne Reuber , Robert Creelman , Frederick D. Hempel , Neal L. Gutterson , Roger Canales
IPC: A01H1/00
CPC classification number: C07K14/415 , C12N15/8261 , Y02A40/146
Abstract: The present invention provides nucleic acid constructs, including plasmids, expression vectors or expression cassettes comprising polynucleotides encoding CCAAT-binding transcription factor polypeptides that have the ability to increase a plant's tolerance to abiotic stress. Polynucleotides encoding functional CCAAT-binding transcription factors were incorporated into expression vectors, introduced into plants, and ectopically expressed. The encoded polypeptides of the invention significantly increased the cold and water deficit tolerance of the transgenic plants, as compared to tolerance to these stresses of control plants.
Abstract translation: 本发明提供核酸构建体,包括质粒,表达载体或表达盒,其包含编码具有增加植物对非生物胁迫耐受性的能力的编码CCAAT结合转录因子多肽的多核苷酸。 将编码功能性CCAAT结合转录因子的多核苷酸掺入表达载体中,引入植物中并进行异位表达。 与对照植物的这些胁迫的耐受性相比,本发明的编码多肽显着增加了转基因植物的冷和缺水耐受性。
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公开(公告)号:US20130198909A1
公开(公告)日:2013-08-01
申请号:US13758380
申请日:2013-02-04
Applicant: Jose Luis Riechmann , Robert Creelman , Oliver Ratcliffe , T. Lynne Reuber , Neal I. Gutterson
Inventor: Jose Luis Riechmann , Robert Creelman , Oliver Ratcliffe , T. Lynne Reuber , Neal I. Gutterson
IPC: C12N15/11
CPC classification number: C12N15/8273 , A01H1/02 , C07K14/415 , C12N15/11 , C12N15/8261 , C12N15/8271 , Y02A40/146
Abstract: The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.
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公开(公告)号:US20120137382A1
公开(公告)日:2012-05-31
申请号:US13244288
申请日:2011-09-24
Applicant: Peter P. Repetti , T. Lynne Reuber , Oliver Ratcliffe , Karen S. Century , Katherine Krolikowski , Robert A. Creelman , Frederick D. Hempel , Roderick W. Kumimoto , Luc J. Adam , Neal I. Gutterson , Roger D. Canales , Emily L. Queen , Jennifer M. Costa
Inventor: Peter P. Repetti , T. Lynne Reuber , Oliver Ratcliffe , Karen S. Century , Katherine Krolikowski , Robert A. Creelman , Frederick D. Hempel , Roderick W. Kumimoto , Luc J. Adam , Neal I. Gutterson , Roger D. Canales , Emily L. Queen , Jennifer M. Costa
CPC classification number: C07K14/415 , C12N15/8261 , C12N15/8271 , Y02A40/146
Abstract: Transcription factor polynucleotides and polypeptides incorporated into nucleic acid constructs, including expression vectors, have been introduced into plants and were ectopically expressed. Transgenic plants transformed with many of these constructs have been shown to be more resistant to disease (in some cases, to more than one pathogen), or more tolerant to an abiotic stress (in some cases, to more than one abiotic stress). The abiotic stress may include, for example, salt, hyperosmotic stress, water deficit, heat, cold, drought, or low nutrient conditions.
Abstract translation: 掺入核酸构建体(包括表达载体)的转录因子多核苷酸和多肽已经被引入植物中并被异位表达。 已经显示用许多这些构建体转化的转基因植物对疾病(在一些情况下,多于一种病原体)更耐受,或更耐受非生物胁迫(在某些情况下,对多于一种非生物胁迫)。 非生物胁迫可包括例如盐,高渗胁迫,缺水,热,冷,干旱或低营养条件。
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公开(公告)号:US20120131691A1
公开(公告)日:2012-05-24
申请号:US13322136
申请日:2010-05-17
Applicant: Peter P. Repetti , Rajnish Khanna , Hans E. Holtan , T. Lynne Reuber , Oliver J. Ratcliffe
Inventor: Peter P. Repetti , Rajnish Khanna , Hans E. Holtan , T. Lynne Reuber , Oliver J. Ratcliffe
CPC classification number: C12N15/8202
Abstract: Light-regulated promoter sequences were identified that respond to differential light conditions and so can be used to regulate gene expression in a light- or dark-inducible manner. These promoters may be used to produce transgenic plants that have an altered trait relative to control plants. In preferred embodiments, the transgenic plants with the improved traits are morphologically and/or developmentally similar to control plants (examples of the latter include wild-type or non-transformed plants of the same species). Any of these light-regulated promoters may be incorporated into a nucleic acid construct that comprises a polynucleotide regulated by one such promoter and that encodes a polypeptide or RNA molecule that, when ectopically expressed, confers an improved trait in plants.
Abstract translation: 鉴定了响应差异光条件的光调节启动子序列,因此可以以浅或暗诱导的方式用于调节基因表达。 这些启动子可用于产生相对于对照植物具有改变的性状的转基因植物。 在优选的实施方案中,具有改善的性状的转基因植物在形态上和/或发育上与对照植物相似(后者的实例包括相同物种的野生型或非转化植物)。 这些光调节启动子中的任何一个可以掺入到包含由一个这样的启动子调控的多核苷酸的核酸构建体中,并且编码多肽或RNA分子,所述多肽或RNA分子当异位表达时赋予植物中改良的性状。
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公开(公告)号:US08110725B2
公开(公告)日:2012-02-07
申请号:US12338024
申请日:2008-12-18
Applicant: Jose Luis Riechmann , Jacqueline E. Heard , Oliver Ratcliffe , T. Lynne Reuber
Inventor: Jose Luis Riechmann , Jacqueline E. Heard , Oliver Ratcliffe , T. Lynne Reuber
CPC classification number: C12N15/8201 , C07K14/415 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8263 , C12N15/8266 , C12N15/8267 , C12N15/827 , C12N15/8271 , C12N15/8273 , C12N15/8275 , C12N15/8279 , C12N15/8282 , C12N15/8291 , C12N15/8293 , Y02A40/146
Abstract: The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.
Abstract translation: 本发明涉及植物转录因子多肽,编码它们的多核苷酸,来自各种植物物种的同系物,以及使用多核苷酸和多肽产生与参照植物相比具有有利特性的转基因植物的方法。 与这些多核苷酸和多肽相关的序列信息也可以用于生物信息学检索方法,并且也被公开。
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公开(公告)号:US20110179520A1
公开(公告)日:2011-07-21
申请号:US13009039
申请日:2011-01-19
Applicant: Suqin CAI , Hans E. Holtan , Peter P. Repetti , T. Lynne Reuber
Inventor: Suqin CAI , Hans E. Holtan , Peter P. Repetti , T. Lynne Reuber
CPC classification number: C12N15/8226 , C12N15/8223 , C12N15/8225 , C12N15/8229
Abstract: Tissue-enhanced promoter sequences were identified that enhance expression of a polypeptide in one or more plant tissues. These promoters may be used to produce transgenic plants that have an altered trait relative to control plants. In preferred embodiments, the transgenic plants with the improved traits are morphologically and/or developmentally similar to control plants (examples of the latter include wild-type or non-transformed plants of the same species). Any of these tissue-enhanced promoters may be incorporated into a nucleic acid construct that comprises a polynucleotide regulated by one such promoter and that encodes a polypeptide or RNA molecule that, when ectopically expressed, confers an improved trait in plants.
Abstract translation: 鉴定出增强一个或多个植物组织中多肽表达的组织增强的启动子序列。 这些启动子可用于产生相对于对照植物具有改变的性状的转基因植物。 在优选的实施方案中,具有改善的性状的转基因植物在形态上和/或发育上与对照植物相似(后者的实例包括相同物种的野生型或非转化植物)。 这些组织增强的启动子中的任何一个可以并入包含由一个这样的启动子调节的多核苷酸的核酸构建体,并且编码多肽或RNA分子,当异位表达时赋予植物中改良的性状。
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公开(公告)号:US20110119789A1
公开(公告)日:2011-05-19
申请号:US12977763
申请日:2010-12-23
Applicant: Robert A. Creelman , Luc J. Adam , Jose Luis Riechmann , Jacqueline E. Heard , Omaira Pineda , Cai-Zhong Jiang , Oliver J. Ratcliffe , T. Lynne Reuber
Inventor: Robert A. Creelman , Luc J. Adam , Jose Luis Riechmann , Jacqueline E. Heard , Omaira Pineda , Cai-Zhong Jiang , Oliver J. Ratcliffe , T. Lynne Reuber
CPC classification number: C12N15/8247 , C07K14/415 , C12N15/8201 , C12N15/8241 , C12N15/8251 , C12N15/8261 , C12N15/8266 , C12N15/8269 , C12N15/827 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/8282 , C12N15/8293 , Y02A40/146
Abstract: The invention is directed to transgenic plants transformed with nucleic acids that encode a plant transcription factor that increases the transgenic plant's size and yield and/or delays flowering in the plant, and methods of using and producing the transgenic plants.
Abstract translation: 本发明涉及用编码植物转录因子的核酸转化的转基因植物,所述植物转录因子增加转基因植物的大小和植物的产量和/或延迟开花,以及使用和产生转基因植物的方法。
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公开(公告)号:US20100162427A1
公开(公告)日:2010-06-24
申请号:US12702109
申请日:2010-02-08
Applicant: Jose Luis RIECHMANN , Cai-Zhong Jiang , Jacqueline E. Heard , Robert Creelman , Oliver Ratcliffe , Luc J. Adam , T. Lynne Reuber , Peter P. Repetti , Roderick W. Kumimoto , Neal I. Gutterson , Marsha L. Pilgrim , Omaira Pineda , James Zhang , Gregory Nadzan
Inventor: Jose Luis RIECHMANN , Cai-Zhong Jiang , Jacqueline E. Heard , Robert Creelman , Oliver Ratcliffe , Luc J. Adam , T. Lynne Reuber , Peter P. Repetti , Roderick W. Kumimoto , Neal I. Gutterson , Marsha L. Pilgrim , Omaira Pineda , James Zhang , Gregory Nadzan
CPC classification number: C07K14/415 , C12N15/8261 , Y02A40/146
Abstract: The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.
Abstract translation: 本发明涉及植物转录因子多肽,编码它们的多核苷酸,来自各种植物物种的同系物,以及使用多核苷酸和多肽产生与参照植物相比具有有利特性的转基因植物的方法。 与这些多核苷酸和多肽相关的序列信息也可以用于生物信息学检索方法,并且也被公开。
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49.
公开(公告)号:US07601893B2
公开(公告)日:2009-10-13
申请号:US11725235
申请日:2007-03-16
Applicant: T. Lynne Reuber , Jose Luis Riechmann , Jacqueline E. Heard , Cai-Zhong Jiang , Luc Adam , Arnold N. Dubell , Oliver Ratcliffe , Omaira Pineda , Guo-Liang Yu , Pierre Broun
Inventor: T. Lynne Reuber , Jose Luis Riechmann , Jacqueline E. Heard , Cai-Zhong Jiang , Luc Adam , Arnold N. Dubell , Oliver Ratcliffe , Omaira Pineda , Guo-Liang Yu , Pierre Broun
CPC classification number: C07K14/415 , C12N15/8241 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8275 , C12N15/8282 , Y02A40/146
Abstract: The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.
Abstract translation: 本发明涉及植物转录因子多肽,编码它们的多核苷酸,来自各种植物物种的同系物,以及使用多核苷酸和多肽产生与参照植物相比具有有利特性的转基因植物的方法。 与这些多核苷酸和多肽相关的序列信息也可以用于生物信息学检索方法,并且也被公开。
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公开(公告)号:US07511190B2
公开(公告)日:2009-03-31
申请号:US10374780
申请日:2003-02-25
Applicant: Robert A. Creelman , Oliver Ratcliffe , T. Lynne Reuber , James Zhang , Gregory Nadzan
Inventor: Robert A. Creelman , Oliver Ratcliffe , T. Lynne Reuber , James Zhang , Gregory Nadzan
CPC classification number: C12N15/8201 , C07K14/415 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8263 , C12N15/8266 , C12N15/8267 , C12N15/827 , C12N15/8271 , C12N15/8273 , C12N15/8275 , C12N15/8279 , C12N15/8282 , C12N15/8291 , C12N15/8293 , Y02A40/146
Abstract: The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.
Abstract translation: 本发明涉及植物转录因子多肽,编码它们的多核苷酸,来自各种植物物种的同系物,以及使用多核苷酸和多肽产生与参照植物相比具有有利特性的转基因植物的方法。 与这些多核苷酸和多肽相关的序列信息也可以用于生物信息学检索方法,并且也被公开。
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