公开(公告)号：US11181464B2

公开(公告)日：2021-11-23

申请号：US16422630

申请日：2019-05-24

Applicant: Becton, Dickinson and Company

Inventor： Timothy Petersen ,  Eric D. Diebold ,  Jianying Cao ,  Bernard Johanna Verwer

IPC: G01N15/14 ,  G01N15/10

Abstract: Systems for differentially detecting light (e.g., in a flow stream) are described. Light detection systems according to certain embodiments include a plurality of photodetectors, an amplifier component and an electronic switch component having a plurality of switches in electrical communication with the plurality of photodetectors and the amplifier component. Systems and methods for differentially detecting light and optimizing the measurement of light emitted by a sample (e.g., in a flow stream) are also described. Kits having a photodetector array, an amplifier component and an electronic switch component are also provided.

公开(公告)号：US20210356381A1

公开(公告)日：2021-11-18

申请号：US17386192

申请日：2021-07-27

Applicant: Becton, Dickinson and Company

Inventor： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

IPC: G01N15/14 ,  G01N21/64

Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data.. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds, In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics of a component of the cell. In some embodiments in which more than two frequency-shifted optical frequencies are employed, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.

公开(公告)号：US20210199559A1

公开(公告)日：2021-07-01

申请号：US17201738

申请日：2021-03-15

Applicant: Becton, Dickinson and Company

Inventor： Jizuo Zou ,  Matthew Bahr ,  Eric D. Diebold

IPC: G01N15/14 ,  G01N21/53 ,  G01J1/04 ,  G01J1/42

Abstract: Light detection systems for measuring light (e.g., in a flow stream) are described. Light detection systems according to embodiments include a light scatter detector, a brightfield photodetector and an optical adjustment component configured to convey light to the light scatter detector and to the brightfield photodetector. Systems and methods for measuring light emitted by a sample (e.g., in a flow stream) and kits having a light scatter detector, a brightfield photodetector and a beam splitter component are also provided.

公开(公告)号：US20200300749A1

公开(公告)日：2020-09-24

申请号：US16817103

申请日：2020-03-12

Applicant: Becton, Dickinson and Company

Inventor： Jizuo Zou ,  Matthew Bahr ,  Eric D. Diebold

IPC: G01N15/14

Abstract: Light detection systems for measuring light (e.g., in a flow stream) are described. Light detection systems according to embodiments include a light scatter detector, a brightfield photodetector and an optical adjustment component configured to convey light to the light scatter detector and to the brightfield photodetector. Systems and methods for measuring light emitted by a sample (e.g., in a flow stream) and kits having a light scatter detector, a brightfield photodetector and a beam splitter component are also provided.

公开(公告)号：US10006852B2

公开(公告)日：2018-06-26

申请号：US15698506

申请日：2017-09-07

Applicant: Becton, Dickinson and Company

Inventor： Eric D. Diebold ,  Keegan Owsley ,  Matthew Bahr

IPC: G01N21/00 ,  G01N15/14 ,  G02F1/33 ,  H01S3/00

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/145 ,  G02F1/33 ,  G02F2203/18 ,  G02F2203/24 ,  H01S3/0071

Abstract: Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.