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公开(公告)号：US11268890B2
公开(公告)日：2022-03-08
申请号：US16817331
申请日：2020-03-12
Applicant: Becton, Dickinson and Company
Inventor： Matthew Bahr , Eric D. Diebold , Jonathan Lin , Keegan Owsley
Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image of an object in the flow stream in an interrogation region and determining whether the object in the flow stream is an aggregate based on the generated image. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image of an object in a flow stream and to determine whether the object is an aggregate are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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42.发明申请公开(公告)号：US20210404943A1
公开(公告)日：2021-12-30
申请号：US17226420
申请日：2021-04-09
Applicant: Becton, Dickinson and Company
Inventor： Jizuo Zou , Jorge Manzarraga , Eric D. Diebold
Abstract: Aspects of the disclosure include methods for generating angularly deflected laser beams for irradiating a sample in a flow stream. Methods according to certain embodiments include generating a first set of angularly deflected laser beams and a second set of angularly deflected laser beams, propagating the first set of angularly deflected laser beams along a different optical path from the second set of angularly deflected laser beams, combining the first set of angularly deflected laser beams with the second set of angularly deflected laser beams and directing the combined sets of laser beams onto a sample in a flow stream and detecting light from the sample. Systems having a laser, an acousto-optic device and an optical adjustment component configured to generate a first set of angularly deflected laser beams and a second set of angularly deflected laser beams are also described.
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Patent Agency Ranking
公开(公告)号：US11181464B2
公开(公告)日：2021-11-23
申请号：US16422630
申请日：2019-05-24
Applicant: Becton, Dickinson and Company
Inventor： Timothy Petersen , Eric D. Diebold , Jianying Cao , Bernard Johanna Verwer
Abstract: Systems for differentially detecting light (e.g., in a flow stream) are described. Light detection systems according to certain embodiments include a plurality of photodetectors, an amplifier component and an electronic switch component having a plurality of switches in electrical communication with the plurality of photodetectors and the amplifier component. Systems and methods for differentially detecting light and optimizing the measurement of light emitted by a sample (e.g., in a flow stream) are also described. Kits having a photodetector array, an amplifier component and an electronic switch component are also provided.
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公开(公告)号：US20210356381A1
公开(公告)日：2021-11-18
申请号：US17386192
申请日：2021-07-27
Applicant: Becton, Dickinson and Company
Inventor： Eric D. Diebold , Keegan Owsley , Jonathan Lin
Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data.. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds, In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics of a component of the cell. In some embodiments in which more than two frequency-shifted optical frequencies are employed, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.
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公开(公告)号：US20210199559A1
公开(公告)日：2021-07-01
申请号：US17201738
申请日：2021-03-15
Applicant: Becton, Dickinson and Company
Inventor： Jizuo Zou , Matthew Bahr , Eric D. Diebold
Abstract: Light detection systems for measuring light (e.g., in a flow stream) are described. Light detection systems according to embodiments include a light scatter detector, a brightfield photodetector and an optical adjustment component configured to convey light to the light scatter detector and to the brightfield photodetector. Systems and methods for measuring light emitted by a sample (e.g., in a flow stream) and kits having a light scatter detector, a brightfield photodetector and a beam splitter component are also provided.
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公开(公告)号：US20200300749A1
公开(公告)日：2020-09-24
申请号：US16817103
申请日：2020-03-12
Applicant: Becton, Dickinson and Company
Inventor： Jizuo Zou , Matthew Bahr , Eric D. Diebold
IPC: G01N15/14
Abstract: Light detection systems for measuring light (e.g., in a flow stream) are described. Light detection systems according to embodiments include a light scatter detector, a brightfield photodetector and an optical adjustment component configured to convey light to the light scatter detector and to the brightfield photodetector. Systems and methods for measuring light emitted by a sample (e.g., in a flow stream) and kits having a light scatter detector, a brightfield photodetector and a beam splitter component are also provided.
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公开(公告)号：US10006852B2
公开(公告)日：2018-06-26
申请号：US15698506
申请日：2017-09-07
Applicant: Becton, Dickinson and Company
Inventor： Eric D. Diebold , Keegan Owsley , Matthew Bahr
CPC classification number: G01N15/1434 , G01N15/1459 , G01N2015/1006 , G01N2015/145 , G02F1/33 , G02F2203/18 , G02F2203/24 , H01S3/0071
Abstract: Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.
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