公开(公告)号：US08921055B2

公开(公告)日：2014-12-30

申请号：US14044559

申请日：2013-10-02

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman

IPC: G01N33/53 ,  G01N33/569 ,  G01N33/68

CPC classification number: G01N33/56972 ,  G01N33/56911 ,  G01N33/56961 ,  G01N33/56966 ,  G01N33/6854 ,  G01N2458/00 ,  G01N2469/10

Abstract: In some cases, the described systems and methods include obtaining a cell sample containing multiple antibody-producing cells. In such cases, the cells can be tagged with a cross-linking reagent having a first portion configured to bind to a marker on the antibody-producing cells and a second portion configured to bind to an antigen of interest. In some instances, the tagged antibody-producing cells are exposed to the antigen of interest such that the antigen becomes bound to the cells. In some such instances, the antibody-producing cells are also allowed to produce an antibody, such that a portion of the antibody-producing cells produce an antigen-specific antibody that binds to the antigen of interest. To identify cells that produce the antigen-specific antibody, the tagged cells can be exposed to a labeled secondary antibody that is configured to bind to the antigen-specific antibody. Other implementations are also described.

Abstract translation: 在一些情况下，所描述的系统和方法包括获得含有多个产生抗体的细胞的细胞样品。 在这种情况下，可以用交联试剂对细胞进行标记，所述交联试剂具有构建成结合抗体产生细胞上的标记的第一部分和被配置成与感兴趣的抗原结合的第二部分。 在一些情况下，标记的产生抗体的细胞暴露于感兴趣的抗原，使得抗原与细胞结合。 在一些这样的情况下，也允许产生抗体的细胞产生抗体，使得产生一部分抗体的细胞产生与感兴趣的抗原结合的抗原特异性抗体。 为了鉴定产生抗原特异性抗体的细胞，标记的细胞可以暴露于被配置为结合抗原特异性抗体的标记的二抗。 还描述了其他实现。

公开(公告)号：US20140116881A1

公开(公告)日：2014-05-01

申请号：US14060117

申请日：2013-10-22

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Igor Y. Khandros ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Ming C. Wu

IPC: G01N27/447

CPC classification number: G01N27/44791 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0454 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26

Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.

Abstract translation: 可以确定性地选择各个生物微物体并将其移动到微流体装置中的保持笔中。 可以向笔提供第一液体介质的流动。 物理笔可被构造成妨碍第一介质直接流入笔中的第二介质，同时允许第一介质和第二介质的扩散混合。 虚拟笔可以允许中等到笔的多个笔的共同流动。