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1.发明授权公开(公告)号：US11971409B2
公开(公告)日：2024-04-30
申请号：US16892642
申请日：2020-06-04
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Mark P. White , Xiaohua Wang , Minha Park , Guido K. Stadler , Randall D. Lowe, Jr. , Xiao Guan Radstrom , Jason M. McEwen , Gang F. Wang , George L. Fox , Peggy A. Radel
IPC: B01L3/00 , C07K16/00 , C07K16/28 , C07K16/30 , C12Q1/6886 , G01N33/50 , G01N33/543 , G01N33/569 , G01N33/574
CPC classification number: G01N33/574 , B01L3/502761 , C07K16/00 , C07K16/2887 , C07K16/30 , C12Q1/6886 , G01N33/5047 , G01N33/505 , G01N33/5052 , G01N33/54366 , G01N33/56972 , B01L2200/0647 , G01N2800/7028
Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.
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公开(公告)号：US20210069698A1
公开(公告)日：2021-03-11
申请号：US16928225
申请日：2020-07-14
Applicant: Berkeley Lights, Inc.
Inventor： Xiao Guan , Mark P. White , Jason M. McEwen , Gang F. Wang , Kevin T. Chapman , Xiaohua Wang , Christine E. Sun
Abstract: Functional assays using reporter cell assays are described which probe the activity of at least one cell of interest. The ability to probe at least one cell is provided by using the microfluidic methods, devices and kits described herein. Assays combining both reporter cell signaling as well as binding assay signaling for at least one cell is also described herein.
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3.发明申请公开(公告)号：US20170276679A1
公开(公告)日：2017-09-28
申请号：US15406289
申请日：2017-01-13
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Mark P. White , Xiaohua Wang , Minha Park , Guido K. Stadler , Randall D. Lowe, Jr. , Xiao Guan , Jason M. McEwen , Gang Wang , George L. Fox , Peggy A. Radel
IPC: G01N33/574 , C12Q1/68 , G01N33/543 , B01L3/00
Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.
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公开(公告)号：US09588117B2
公开(公告)日：2017-03-07
申请号：US14583351
申请日：2014-12-26
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman
IPC: G01N33/569 , G01N33/68
CPC classification number: G01N33/56972 , G01N33/56911 , G01N33/56961 , G01N33/56966 , G01N33/6854 , G01N2458/00 , G01N2469/10
Abstract: In some cases, the described systems and methods include obtaining a cell sample containing multiple antibody-producing cells. In such cases, the cells can be tagged with a cross-linking reagent having a first portion configured to bind to a marker on the antibody-producing cells and a second portion configured to bind to an antigen of interest. In some instances, the tagged antibody-producing cells are exposed to the antigen of interest such that the antigen becomes bound to the cells. In some such instances, the antibody-producing cells are also allowed to produce an antibody, such that a portion of the antibody-producing cells produce an antigen-specific antibody that binds to the antigen of interest. To identify cells that produce the antigen-specific antibody, the tagged cells can be exposed to a labeled secondary antibody that is configured to bind to the antigen-specific antibody. Other implementations are also described.
Abstract translation: 在一些情况下，所描述的系统和方法包括获得含有多个产生抗体的细胞的细胞样品。 在这种情况下，可以用交联试剂对细胞进行标记，所述交联试剂具有构建成结合抗体产生细胞上的标记的第一部分和被配置成与感兴趣的抗原结合的第二部分。 在一些情况下，标记的产生抗体的细胞暴露于感兴趣的抗原，使得抗原与细胞结合。 在一些这样的情况下，也允许产生抗体的细胞产生抗体，使得产生一部分抗体的细胞产生与感兴趣的抗原结合的抗原特异性抗体。 为了鉴定产生抗原特异性抗体的细胞，标记的细胞可以暴露于被配置为结合抗原特异性抗体的标记的二抗。 还描述了其他实现。
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公开(公告)号：US20160338347A1
公开(公告)日：2016-11-24
申请号：US15136777
申请日：2016-04-22
Applicant: BERKELEY LIGHTS, INC.
Inventor： Mark P. White , Kevin T. Chapman , Andrew W. McFarland , Eric D. Hobbs , Randall D. Lowe, JR.
CPC classification number: A01N1/0284 , A01N1/0221 , A01N1/0263 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2300/021 , B01L2300/024 , B01L2300/0864 , B01L2300/16 , B01L2300/163 , B01L2300/18 , B01L2300/1894 , C12M47/04
Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.
Abstract translation: 一种处理和存储生物细胞的方法包括将可流动介质引入微流体装置，所述可流动介质包括生物细胞; 在微流体装置的一个或多个隔离区域中从可流动介质中隔离一个或多个生物细胞; 以及冷冻微流体装置，其中包含一个或多个生物细胞。
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Patent Agency Ranking
公开(公告)号：US20150111784A1
公开(公告)日：2015-04-23
申请号：US14583351
申请日：2014-12-26
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman
IPC: G01N33/569
CPC classification number: G01N33/56972 , G01N33/56911 , G01N33/56961 , G01N33/56966 , G01N33/6854 , G01N2458/00 , G01N2469/10
Abstract: In some cases, the described systems and methods include obtaining a cell sample containing multiple antibody-producing cells. In such cases, the cells can be tagged with a cross-linking reagent having a first portion configured to bind to a marker on the antibody-producing cells and a second portion configured to bind to an antigen of interest. In some instances, the tagged antibody-producing cells are exposed to the antigen of interest such that the antigen becomes bound to the cells. In some such instances, the antibody-producing cells are also allowed to produce an antibody, such that a portion of the antibody-producing cells produce an antigen-specific antibody that binds to the antigen of interest. To identify cells that produce the antigen-specific antibody, the tagged cells can be exposed to a labeled secondary antibody that is configured to bind to the antigen-specific antibody. Other implementations are also described.
Abstract translation: 在一些情况下，所描述的系统和方法包括获得含有多个产生抗体的细胞的细胞样品。 在这种情况下，可以用交联试剂对细胞进行标记，所述交联试剂具有构建成结合抗体产生细胞上的标记的第一部分和被配置成与感兴趣的抗原结合的第二部分。 在一些情况下，标记的产生抗体的细胞暴露于感兴趣的抗原，使得抗原与细胞结合。 在一些这样的情况下，也允许产生抗体的细胞产生抗体，使得产生一部分抗体的细胞产生与感兴趣的抗原结合的抗原特异性抗体。 为了鉴定产生抗原特异性抗体的细胞，标记的细胞可以暴露于被配置为结合抗原特异性抗体的标记的二抗。 还描述了其他实现。
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公开(公告)号：US20240027396A1
公开(公告)日：2024-01-25
申请号：US18324026
申请日：2023-05-25
Applicant: BERKELEY LIGHTS, INC.
Inventor： Kevin T. Chapman , Igor Y. Khandros , Gaetan L. Mathieu , J. Tanner Nevill , Ming C. Wu
IPC: G01N27/447 , B01L3/00 , B03C5/00 , B03C5/02
CPC classification number: G01N27/44791 , B01L3/502761 , B03C5/005 , B03C5/026 , B01L2200/0668 , B01L2300/0816 , B01L2300/0819 , B01L2300/0877 , B01L2400/0424 , B01L2400/086 , B03C2201/26 , B01L2400/0454
Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.
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公开(公告)号：US11305283B2
公开(公告)日：2022-04-19
申请号：US16509166
申请日：2019-07-11
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White , M. Jimena Loureiro
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
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公开(公告)号：US20210368781A1
公开(公告)日：2021-12-02
申请号：US17228058
申请日：2021-04-12
Applicant: BERKELEY LIGHTS, INC.
Inventor： Mark P. White , Kevin T. Chapman , Andrew W. McFarland , Eric D. Hobbs , Randall D. Lowe, JR.
Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.
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公开(公告)号：US10751715B1
公开(公告)日：2020-08-25
申请号：US15136481
申请日：2016-04-22
Applicant: Berkeley Lights, Inc.
Inventor： Xiao Guan , Mark P. White , Jason M. McEwen , Gang F. Wang , Kevin T. Chapman , Xiaohua Wang , Christine E. Sun
Abstract: Functional assays using reporter cell assays are described which probe the activity of at least one cell of interest. The ability to probe at least one cell is provided by using the microfluidic methods, devices and kits described herein. Assays combining both reporter cell signaling as well as binding assay signaling for at least one cell is also described herein.
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