公开(公告)号：US20240027396A1

公开(公告)日：2024-01-25

申请号：US18324026

申请日：2023-05-25

Applicant: BERKELEY LIGHTS, INC.

Inventor： Kevin T. Chapman ,  Igor Y. Khandros ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Ming C. Wu

IPC: G01N27/447 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02

CPC classification number: G01N27/44791 ,  B01L3/502761 ,  B03C5/005 ,  B03C5/026 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/086 ,  B03C2201/26 ,  B01L2400/0454

Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.

公开(公告)号：US20180154362A1

公开(公告)日：2018-06-07

申请号：US15825689

申请日：2017-11-29

Applicant: The University of Chicago

Inventor： Rustem F. Ismagilov ,  Joshua David Tice ,  Helen Song Baca ,  Lewis Spencer Roach

IPC: B01L3/00 ,  C12Q1/44 ,  B01F3/08 ,  B01F5/06 ,  B01F13/00 ,  C12P19/34 ,  B01J19/00 ,  G01N15/14 ,  G01N35/08 ,  B01L7/00 ,  B82Y30/00

CPC classification number: B01L3/502784 ,  B01F3/0861 ,  B01F5/0403 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0071 ,  B01F2215/0037 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00736 ,  B01J2219/0074 ,  B01J2219/00756 ,  B01J2219/00783 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00867 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00975 ,  B01J2219/00977 ,  B01L3/502746 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0424 ,  B01L2400/0448 ,  B01L2400/0454 ,  B01L2400/0487 ,  B82Y30/00 ,  C12P19/34 ,  C12Q1/44 ,  G01N15/1404 ,  G01N15/1484 ,  G01N35/08 ,  G01N35/085 ,  G01N2015/1409 ,  Y02A90/26 ,  Y10T436/118339 ,  Y10T436/12 ,  Y10T436/2575

Abstract: The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

公开(公告)号：US20180120255A1

公开(公告)日：2018-05-03

申请号：US15657202

申请日：2017-07-23

Applicant: TECHTRON TECHNOLOGY CO., LTD.

Inventor： Hung-Wei WU ,  Cheng-Yuan HUNG ,  Chang-Sin YE

IPC: G01N27/447 ,  B03C5/00 ,  B03C5/02

CPC classification number: G01N27/447 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0454 ,  B03C5/005 ,  B03C5/024 ,  B03C5/026 ,  B03C2201/26 ,  G01N15/0266 ,  G01N2015/0053 ,  G01N2015/008 ,  G01N2015/0288

Abstract: A light induced dielectrophoresis (LIDEP) device is configured to perform a sorting process on a liquid including plural first micro-particles and plural second micro-particles. The LIDEP device includes a LIDEP chip and an opaque cartridge. The LIDEP chip includes a first electrode layer, a second electrode layer, a semiconductor layer, and a flow channel layer. The flow channel layer defines a first channel, a second channel and a third channel intersected at a confluence. The first channel is configured to guide the liquid. The flow channel layer further defines a projection region including the confluence. A patterned light source is projected on the projection region, thereby guiding the first micro-particles and the second micro-particles located within the confluence to move toward the second channel and the third channel, respectively. The opaque cartridge covers the LIDEP chip and has an opening. The vertical projection of the opening overlaps the projection region.

公开(公告)号：US09889445B2

公开(公告)日：2018-02-13

申请号：US14521447

申请日：2014-10-22

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M Jimena Loureiro

IPC: B01L3/00 ,  G01N33/68 ,  G01N33/50 ,  G01N33/543 ,  G01N15/14 ,  B03C5/00 ,  B03C5/02

CPC classification number: B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0454 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N15/1484 ,  G01N33/5023 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/5052 ,  G01N33/54313 ,  G01N33/6854

Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

公开(公告)号：US09744533B2

公开(公告)日：2017-08-29

申请号：US14965721

申请日：2015-12-10

Applicant: Berkeley Lights, Inc.

Inventor： Keith J. Breinlinger ,  Eric D. Hobbs ,  Daniele Malleo ,  J. Tanner Nevill ,  Mark P. White

IPC: B01L3/00 ,  G01N1/40

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0454 ,  B01L2400/0457 ,  B01L2400/086 ,  G01N1/4077 ,  G01N2001/4083

Abstract: A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.

公开(公告)号：US20170232440A1

公开(公告)日：2017-08-17

申请号：US15586896

申请日：2017-05-04

Applicant: The University of Chicago

Inventor： Rustem F. Ismagilov ,  Joshua David Tice ,  Helen Song Baca ,  Lewis Spencer Roach

IPC: B01L3/00 ,  B01F3/08 ,  B01F13/00 ,  B01F5/06 ,  B01J19/00 ,  B01L7/00

CPC classification number: B01L3/502784 ,  B01F3/0861 ,  B01F5/0403 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0071 ,  B01F2215/0037 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00736 ,  B01J2219/0074 ,  B01J2219/00756 ,  B01J2219/00783 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00867 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00975 ,  B01J2219/00977 ,  B01L3/502746 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0424 ,  B01L2400/0448 ,  B01L2400/0454 ,  B01L2400/0487 ,  B82Y30/00 ,  C12P19/34 ,  C12Q1/44 ,  G01N15/1404 ,  G01N15/1484 ,  G01N35/08 ,  G01N35/085 ,  G01N2015/1409 ,  Y02A90/26 ,  Y10T436/118339 ,  Y10T436/12 ,  Y10T436/2575

Abstract: The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

公开(公告)号：US20170080425A1

公开(公告)日：2017-03-23

申请号：US15162196

申请日：2016-05-23

Applicant: The General Hospital Corporation

Inventor： Mehmet Toner ,  Dino DiCarlo ,  Jon F. Edd ,  Daniel Irimia

IPC: B01L3/00 ,  G01N15/10 ,  C12M3/06 ,  C12M1/34 ,  F16L41/02 ,  C12M1/00

CPC classification number: B01L3/502776 ,  B01D21/0087 ,  B01D21/265 ,  B01D45/04 ,  B01D45/12 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0883 ,  B01L2400/0409 ,  B01L2400/043 ,  B01L2400/0433 ,  B01L2400/0454 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/084 ,  B33Y80/00 ,  C12M23/16 ,  C12M41/36 ,  C12M47/04 ,  F16L41/02 ,  F17D1/00 ,  G01N15/0255 ,  G01N15/10 ,  G01N2015/0288 ,  G01N2015/1006 ,  G01N2015/1081 ,  Y10T137/85938 ,  Y10T137/85978 ,  Y10T436/117497 ,  Y10T436/2575

Abstract: Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.

公开(公告)号：US09579622B2

公开(公告)日：2017-02-28

申请号：US14507938

申请日：2014-10-07

Applicant: The University of Chicago

Inventor： Rustem F. Ismagilov ,  Joshua David Tice ,  Helen Song ,  Lewis Spencer Roach

IPC: B01L3/00 ,  B01J19/00 ,  B01F5/06 ,  B01F13/00 ,  G01N35/08 ,  C12P19/34 ,  C12Q1/44 ,  B82Y30/00 ,  G01N15/14

CPC classification number: B01L3/502784 ,  B01F3/0861 ,  B01F5/0403 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0071 ,  B01F2215/0037 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00736 ,  B01J2219/0074 ,  B01J2219/00756 ,  B01J2219/00783 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00867 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00975 ,  B01J2219/00977 ,  B01L3/502746 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0424 ,  B01L2400/0448 ,  B01L2400/0454 ,  B01L2400/0487 ,  B82Y30/00 ,  C12P19/34 ,  C12Q1/44 ,  G01N15/1404 ,  G01N15/1484 ,  G01N35/08 ,  G01N35/085 ,  G01N2015/1409 ,  Y02A90/26 ,  Y10T436/118339 ,  Y10T436/12 ,  Y10T436/2575

Abstract: The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

Abstract translation: 本发明提供微制造的底物和在这些底物内进行反应的方法。 反应发生在在载体流体流中输送的塞子中。

公开(公告)号：US20170014794A1

公开(公告)日：2017-01-19

申请号：US15216775

申请日：2016-07-22

Applicant: BioArray Solutions, Ltd.

Inventor： Michael SEUL

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00713 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0663 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0877 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/0454 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/00 ,  C40B40/04 ,  G01N15/1468 ,  G01N27/447 ,  G01N33/54313 ,  G01N33/54386 ,  G01N33/6845 ,  G01N2015/149 ,  G01N2015/1497 ,  G02B3/0012 ,  G02B3/0056 ,  G02B3/0075 ,  H05H3/04 ,  Y10S435/808 ,  Y10S435/81 ,  Y10S435/814 ,  Y10S436/806 ,  Y10S436/808 ,  Y10S436/809 ,  Y10T436/25125 ,  C12Q2565/501 ,  C12Q2563/155 ,  C12Q2565/607

Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components.

Abstract translation: 一种用于在诸如氧化硅的绝缘电极和电解质溶液之间的界面处操作胶体颗粒和生物分子的方法和装置。 表面附近颗粒的光控电动组装取决于三个功能元件的组合：交流电场引起的平面聚集体的组装; 电解质/氧化硅/硅界面的图案化以在组装过程上施加空间控制; 并通过外部照明实现对装配过程的实时控制。 本发明提供一组基本操作，使得能够对几种类型的颗粒和生物分子的平面阵列的创建和放置以及阵列形状和尺寸的操纵进行交互式控制。 本发明使得能够以阵列格式进行诊断测定和生化分析的样品制备和处理以及这些操作的功能整合。 此外，本发明提供了用于产生具有所需性质的材料表面和用于制造表面安装光学部件的步骤。

公开(公告)号：US20160312174A1

公开(公告)日：2016-10-27

申请号：US15134691

申请日：2016-04-21

Applicant: CHRIS D. GEDDES

Inventor： CHRIS D. GEDDES

IPC: C12M1/00 ,  C12M3/06 ,  C12N15/10 ,  C12N13/00

CPC classification number: C12M47/06 ,  B01L3/502707 ,  B01L7/52 ,  B01L2200/10 ,  B01L2400/0454 ,  C12M23/16 ,  C12M45/07 ,  C12N1/066 ,  C12N13/00

Abstract: The present invention provides for lysing systems and methods to rapidly lyse bugs, bacteria, viruses, cells and/or algae in an efficient manner in addition to fragmenting DNA and/or RNA onto smaller pieces. Solutions or gases containing the biological material to be lysed are introduced or pumped (flow) between two or more apexes of metallic triangles with microwave energy focused at the apexes. Subsequently, the rapid heating of fluid between the apexes lyses cells allows for increased collection of the lysate, the inner genetic materials or other components for further purification or isolating thereafter.

Abstract translation: 本发明提供了裂解系统和方法，以便除了将DNA和/或RNA片段分割成较小的碎片之外，还能以有效的方式快速地裂解虫子，细菌，病毒，细胞和/或藻类。 将包含要裂解的生物材料的溶液或气体在两个或多个金属三角顶点之间引入或泵送（流动），微波能量聚焦在顶点处。 随后，在顶点之间的流体的快速加热裂解细胞允许增加收集裂解物，内部遗传物质或其它成分，以进一步纯化或分离。