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公开(公告)号：US08999642B2
公开(公告)日：2015-04-07
申请号：US13387078
申请日：2009-08-25
CPC分类号： C12N15/1068 , C12Q1/6806 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/507 , C12Q2531/107
摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.
摘要翻译： 本发明提供了控制固体支持物表面上不同分子种类的密度的方法。 将不同分子种类的第一混合物连接到固体支持物上，以便以期望的密度附着每种物质，从而产生具有附着捕获分子的衍生化载体。 衍生的载体用不同分子种类的第二混合物处理，其中第二混合物中的不同分子物质特异性地结合到固相载体上的不同捕获分子。 一个或多个捕获分子可以被可逆地修饰，使得捕获分子在分子物质的第二混合物附着之前和之后具有不同的活性。 在具体实施方案中，不同的分子种类是可逆修饰以在扩增反应中具有不同活性的核酸。
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公开(公告)号：US20120122737A1
公开(公告)日：2012-05-17
申请号：US13387078
申请日：2009-08-25
CPC分类号： C12N15/1068 , C12Q1/6806 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/507 , C12Q2531/107
摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.
摘要翻译： 本发明提供了控制固体支持物表面上不同分子种类的密度的方法。 将不同分子种类的第一混合物连接到固体支持物上，以便以期望的密度附着每种物质，从而产生具有附着捕获分子的衍生化载体。 衍生的载体用不同分子种类的第二混合物处理，其中第二混合物中的不同分子物质特异性地结合到固相载体上的不同捕获分子。 一个或多个捕获分子可以被可逆地修饰，使得捕获分子在分子物质的第二混合物附着之前和之后具有不同的活性。 在具体实施方案中，不同的分子种类是可逆修饰以在扩增反应中具有不同活性的核酸。
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公开(公告)号：US20090226975A1
公开(公告)日：2009-09-10
申请号：US12395299
申请日：2009-02-27
IPC分类号： C12P19/34
CPC分类号： C12Q1/6837 , C12Q2565/543
摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.
摘要翻译： 本发明提供了控制固体支持物表面上不同分子种类的密度的方法。 将不同分子种类的第一混合物连接到固体支持物上，以便以期望的密度附着每种物质，从而产生具有附着捕获分子的衍生化载体。 衍生的载体用不同分子种类的第二混合物处理，其中第二混合物中的不同分子物质特异性地结合到固相载体上的不同捕获分子。 一个或多个捕获分子可以被可逆地修饰，使得捕获分子在分子物质的第二混合物附着之前和之后具有不同的活性。 在具体实施方案中，不同的分子种类是可逆修饰以在扩增反应中具有不同活性的核酸。
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公开(公告)号：US20080009420A1
公开(公告)日：2008-01-10
申请号：US11725597
申请日：2007-03-19
申请人： Gary Schroth , David Lloyd , Lu Zhang , Tobias Barrost , Roberto Rigatti , Jonathan Boutell
发明人： Gary Schroth , David Lloyd , Lu Zhang , Tobias Barrost , Roberto Rigatti , Jonathan Boutell
CPC分类号： C12Q1/6848 , C12Q2565/543
摘要： The present invention is directed to a method for isothermal amplification of a plurality of different target nucleic acids, wherein the different target nucleic acids are amplified using universal primers and colonies produced thereby can be distinguished from each other. The method, therefore, generates distinct colonies of amplified nucleic acid sequences that can be analyzed by various means to yield information particular to each distinct colony.
摘要翻译： 本发明涉及一种用于等温扩增多种不同靶核酸的方法，其中使用通用引物扩增不同的靶核酸，并且可以区分由此产生的菌落。 因此，该方法产生扩增的核酸序列的不同集落，其可以通过各种方式分析以产生每个不同集落特有的信息。
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公开(公告)号：US20060281109A1
公开(公告)日：2006-12-14
申请号：US11431939
申请日：2006-05-10
CPC分类号： C12N9/1252 , C12Q1/686 , C12Y207/07007
摘要： Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays.
摘要翻译： 修饰的DNA聚合酶对DNA具有亲和力，使得聚合酶具有在每个反应循环中将一个或多个核苷酸并入到多个单独的DNA模板中的能力。 聚合酶能够在每个反应循环中形成增加数量的生产性聚合酶-DNA复合物。 修饰的聚合酶可用于许多DNA测序应用，特别是在簇阵列的上下文中。
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