摘要:
Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays.
摘要:
The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogues bearing substituents at the 3′ position of the sugar moiety that are larger in size than the naturally occurring 3′ hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
摘要:
The present invention relates to a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5′ ends and at their 3′ ends.
摘要:
A beverage forming system includes an actuator and first and second portions that are movable relative to each other between open and closed positions. The first and second portions include a cartridge receiving portion to accept a cartridge for forming a beverage, e.g., a disposable single serve cartridge. A first linkage member may be fixed to the actuator and physically contact a second linkage member that may be fixed to the first or second portion (or to a frame that supports the first and second portions). Movement of the actuator may cause movement of the first and second linkage members relative to each other, thereby causing relative movement of the first and second portions between open and closed positions.
摘要:
Tiered command distribution is described. In an embodiment, a pipeline architecture includes processor chains of data processors that process control events received from an application interface control. A tier assignment algorithm determines the longest path of data processors through the processor chains to determine a tier allocation for each data processor in the set of processor chains. Each tier includes a data processor from one or more of the processor chains where a first set of data processors in a first tier each receive a control event and process the control event and/or process the data according to the control event before a second set of data processors in a second tier each receive the control event.
摘要:
Strategies are described for performing retry analysis in an environment which involves the transmission of media information from a source module to a target module. In the context of the source module, the retry analysis determines whether the source module should satisfy the retry requests issued by the target module. In the context of the target module, the retry analysis determines whether the target module should generate the retry requests in the first place. Novel request reporting formats are also described. The target module performs analysis to determine what reporting format it should use to convey the retry requests to the source module.
摘要:
Accelerated channel change (ACC) strategies are described for supplying a client module with media information by transitioning between a first delivery of media information provided by first delivery functionality and a second delivery of media information provided by second delivery functionality without exceeding prescribed data rate limitations. The first delivery functionality can represent server-based functionality for delivering a unicast stream of media information at a data rate (or maximum data rate) of some excess amount over the nominal rate of delivery, starting at a selected location within the media information. The second delivery functionality can represent any functionality for delivering the media information at the data rate (or maximum data rate) of the nominal rate of delivery. A join interval separates the first delivery at the excess rate and the second delivery at the nominal rate. In that period, the first delivery functionality can provide media information at a join interval data rate. The join interval data rate, if it is below the nominal data rate, will cause the client module to miss packets of media information. The client module can receive these missing packets by issuing retry requests to the first delivery functionality or to some other entity. The client module can determine the excess amount available by investigating the amount of missing information that occurs at different data rates; or the excess amount can be defined in advance.
摘要:
A system or method selects among multiple tuners to tune a particular channel. A request is received to tune a first channel. In response to this request, a first tuner is assigned to tune the first channel. A request is received to tune a second channel. If the program tuned by the first tuner is not being recorded, the first tuner is assigned to tune the second channel. If the program tuned by the first tuner is being recorded, the second tuner is assigned to tune the second channel.
摘要:
This invention relates to a process for preparing 2-oxazolines which comprises cyclodehydrating a hydroxyalkylamide in the presence of a stannous salt of a carboxylic acid. The 2-oxazolines have a variety of uses such as pharmaceutical intermediates, pharmaceuticals and as stabilizers for resins.
摘要:
A system or method selects among multiple tuners to tune a particular channel. A request is received to tune a first channel. In response to this request, a first tuner is assigned to tune the first channel. A request is received to tune a second channel. If the program tuned by the first tuner is not being recorded, the first tuner is assigned to tune the second channel. If the program tuned by the first tuner is being recorded, the second tuner is assigned to tune the second channel.