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公开(公告)号：US20130115709A1
公开(公告)日：2013-05-09
申请号：US13809179
申请日：2011-06-28
IPC分类号： G01N30/02
CPC分类号： B01D15/361 , C07K1/18
摘要： The present invention relates to a method for running ion exchange chromatography on a media comprising shell beads having an inner porous core and an outer shell, wherein the inner core is provided with ligands whose charge changes with pH and the shell is provided with charged ion exchange ligands, the method comprising the following steps: a) adsorbing sample molecules on the shell ligands at a first pH; b) causing a discharge of the inner core ligands at a second pH by addition of a buffer substance that is able to increase its charge having the same sign/type as that of the core ligands, which at the same time causes release of ions from the inner core ligands and thereby an increase in ionic strength that displaces the sample molecules from the shell ligands i.e. causes an elution.
摘要翻译： 本发明涉及一种用于在包含具有内多孔芯和外壳的壳珠的介质上运行离子交换色谱的方法，其中内核具有其电荷随pH变化并且壳被带有带电离子交换的配体 配体，该方法包括以下步骤：a）在第一pH下将样品分子吸附在壳配体上; b）通过添加能够增加其与核配体相同符号/类型的电荷的缓冲物质，在第二pH下引起内核配体的排出，同时使离子从 内部核心配体，从而使离子强度的增加从壳体配体取代样品分子即引起洗脱。
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公开(公告)号：US20130040809A1
公开(公告)日：2013-02-14
申请号：US13642937
申请日：2011-04-20
IPC分类号： B01J20/281 , B01J20/282 , B01J41/20 , B01J20/30 , B01J39/26
CPC分类号： B01J39/165 , B01J20/28004 , B01J20/286 , B01J20/3242 , B01J20/3293 , B01J39/17 , B01J47/018
摘要： The present invention relates to a method and means to produce chromatography media having improved pressure-flow properties. More closely, the invention relates to bimodal particle size distribution and the use of layer functionalisation as means to change pressure-flow properties of chromatography media. The invention relates to a method for production of chromatography media having improved pressure-flow properties, comprising mixing large beads/particles, comprising an inner core and an outer functionalized shell/lid, with smaller beads/particles, wherein the ratio of the particle size of large and small beads: [D50V for large particles/D50V for small particles]>1.2, and wherein the volume ratio of large and small beads in the column: [Total volume of large beds/Total volume beads] is in the range 0.05-0.9.
摘要翻译： 本发明涉及产生具有改进的压力 - 流动性质的色谱介质的方法和方法。 本发明更紧密地涉及双峰粒度分布和层官能化用作改变色谱介质压力流动性质的手段。 本发明涉及一种用于制备具有改进的压力 - 流动性质的色谱介质的方法，包括将具有较小珠粒/颗粒的包含内核和外部功能化壳/盖的大珠粒/颗粒混合，其中颗粒尺寸 大颗粒和小珠：[D50V为大颗粒/ D50V为小颗粒]> 1.2，其中柱中大小珠的体积比为[大床/总体积珠的总体积]在0.05 -0.9。
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公开(公告)号：US20120309618A1
公开(公告)日：2012-12-06
申请号：US13575750
申请日：2011-02-16
IPC分类号： B01J20/282 , C09K3/00 , B01J39/26 , B01J20/30 , B01J41/20
CPC分类号： B01J20/3293 , B01D15/361 , B01D15/3804 , B01J20/28004 , B01J20/286 , B01J20/3206 , B01J20/3248 , B01J20/3285 , B01J39/26 , B01J41/20 , C07K1/18
摘要： The present invention relates to a method of producing novel chromatography media and use thereof for purification of biomolecules, such as proteins. The chromatography media comprises shell beads having an inner porous core and an outer shell. The method comprises providing buffering ligands in the core of the beads, and providing binding ligands aimed for biomolecule binding in the outer shell of the beads. This method makes it possible to optimize binding properties and buffering properties independently of each other which is especially to advantageous for production of chromatofocusing media.
摘要翻译： 本发明涉及生产新型色谱介质的方法及其用于生物分子如蛋白质的纯化的用途。 色谱介质包括具有内部多孔芯和外壳的壳珠。 该方法包括在珠的核心中提供缓冲配体，并提供靶向用于珠粒外壳中的生物分子结合的结合配体。 该方法使得可以彼此独立地优化结合性质和缓冲性质，这特别有利于生产色谱聚焦介质。
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公开(公告)号：US08574437B2
公开(公告)日：2013-11-05
申请号：US13575750
申请日：2011-02-16
IPC分类号： B01D15/08
CPC分类号： B01J20/3293 , B01D15/361 , B01D15/3804 , B01J20/28004 , B01J20/286 , B01J20/3206 , B01J20/3248 , B01J20/3285 , B01J39/26 , B01J41/20 , C07K1/18
摘要： The present invention relates to a method of producing novel chromatography media and use thereof for purification of biomolecules, such as proteins. The chromatography media comprises shell beads having an inner porous core and an outer shell. The method comprises providing buffering ligands in the core of the beads, and providing binding ligands aimed for biomolecule binding in the outer shell of the beads. This method makes it possible to optimize binding properties and buffering properties independently of each other which is especially to advantageous for production of chromatofocusing media.
摘要翻译： 本发明涉及生产新型色谱介质的方法及其用于生物分子如蛋白质的纯化的用途。 色谱介质包括具有内多孔芯和外壳的壳珠。 该方法包括在珠的核心中提供缓冲配体，并提供靶向用于珠粒外壳中的生物分子结合的结合配体。 该方法使得可以彼此独立地优化结合性质和缓冲性质，这特别有利于生产色谱聚焦介质。
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公开(公告)号：US20060003331A1
公开(公告)日：2006-01-05
申请号：US10535728
申请日：2003-11-17
CPC分类号： B01D15/3828 , B01J45/00 , C07H1/06 , C07H1/08
摘要： The present invention relates to a method of isolating fully thioated single stranded antisense oligonucleotides from a biological solution, which method comprises the steps of contacting the biological solution with an immobilised metal ion adsorption chromatography (IMAC) resin to adsorb the antisense oligonucleotides to said resin and subsequently contacting the resin with an eluent under conditions that provide desorption of the antisense oligonucleotides from said resin, wherein the fully thioated antisense oligonucleotides are separated from incorrectly thioated antisense oligonucleotides in said solution. The invention also relates to the use of an immobilised metal ion adsorption chromatography (IMAC) resin for isolation of fully thioated single stranded antisense oligonucleotides from a biological solution.
摘要翻译： 本发明涉及从生物溶液中分离完全硫酸化的单链反义寡核苷酸的方法，该方法包括使生物溶液与固定金属离子吸附色谱（IMAC）树脂接触以将反义寡核苷酸吸附到所述树脂上， 随后在从所述树脂提供反义寡核苷酸解吸的条件下使树脂与洗脱液接触，其中完全硫代反义寡核苷酸与所述溶液中的错误地硫代反义寡核苷酸分离。 本发明还涉及固定化金属离子吸附色谱（IMAC）树脂从生物溶液中分离完全硫代单链反义寡核苷酸的用途。
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公开(公告)号：US07368561B2
公开(公告)日：2008-05-06
申请号：US10535728
申请日：2003-11-17
CPC分类号： B01D15/3828 , B01J45/00 , C07H1/06 , C07H1/08
摘要： The present invention relates to a method of isolating fully thioated single stranded antisense oligonucleotides from a biological solution, which method comprises the steps of contacting the biological solution with an immobilised metal ion adsorption chromatography (IMAC) resin to adsorb the antisense oligonucleotides to the resin and subsequently contacting the resin with an eluent under conditions that provide desorption of the antisense oligonucleotides from the resin, wherein the fully thioated antisense oligonucleotides are separated from incorrectly thioated antisense oligonucleotides in the solution. The invention also relates to the use of an immobilised metal ion adsorption chromatography (IMAC) resin for isolation of fully thioated single stranded antisense oligonucleotides from a biological solution.
摘要翻译： 本发明涉及一种从生物溶液中分离完全硫代单链反义寡核苷酸的方法，该方法包括使生物溶液与固定金属离子吸附色谱（IMAC）树脂接触以将反义寡核苷酸吸附到树脂上， 随后在提供反义寡核苷酸从树脂解吸的条件下将树脂与洗脱液接触，其中完全硫代反义寡核苷酸与溶液中的错误地硫代反义寡核苷酸分离。 本发明还涉及固定化金属离子吸附色谱（IMAC）树脂从生物溶液中分离完全硫代单链反义寡核苷酸的用途。
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公开(公告)号：US07214659B2
公开(公告)日：2007-05-08
申请号：US10380301
申请日：2001-09-12
IPC分类号： A61K38/00
CPC分类号： A61K38/28
摘要： The present invention relates to the use of proinsulin C-peptide or a variant, derivative or fragment thereof in the manufacture of a medicament for reducing the OTc interval and the use of proinsulin C-peptide or a variant, derivative or fragment thereof in the manufacture of a medicament for reducing the risk of sudden death or ‘dead in bed’ syndrome; particularly in patients suffering from IDDM.
摘要翻译： 本发明涉及胰岛素原C肽或其变体，衍生物或片段在制备用于减少OTc间期的药物中的用途，以及在制造中使用胰岛素原C肽或其变体，衍生物或片段 用于降低猝死或“死于床”综合征的风险的药物; 特别是患有IDDM的患者。
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公开(公告)号：US06610649B2
公开(公告)日：2003-08-26
申请号：US09269439
申请日：1999-07-08
申请人： John Wahren , Bo-Lennart Johansson , Hans Jörnvall
发明人： John Wahren , Bo-Lennart Johansson , Hans Jörnvall
IPC分类号： A61K3816
摘要： The invention features peptides that are fragments of the human insulin C-peptide, which peptides include the sequence ELGGGPGAG or a fragment thereof, or the sequence EGSLQ or a fragment thereof. The peptides have the ability to stimulate Na+K+ATPase activity. Also provided are biomimetic organic compounds exhibiting activation of Na+K+ATPase activity and/or cellular binding to renal tubule cells and fibroblasts. Such peptides and compounds are useful in combating diabetes and diabetic complications, or for stimulating Na+K+ATPase activity.
摘要翻译： 本发明具有作为人胰岛素C肽的片段的肽，该肽包括序列ELGGGPGAG或其片段，或序列EGSLQ或其片段。 肽具有刺激Na + K + ATP酶活性的能力。 还提供了表现出Na + K + ATP酶活性和/或细胞与肾小管细胞和成纤维细胞结合的活化的仿生有机化合物。 这样的肽和化合物可用于治疗糖尿病和糖尿病并发症，或用于刺激Na + K + ATP酶活性。
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