公开(公告)号：US20210148872A1

公开(公告)日：2021-05-20

申请号：US17254466

申请日：2020-05-26

Applicant: ILLUMINA, INC.

Inventor： Tarun Khurana ,  Ali Agah ,  Aathavan Karunakaran ,  Stanley Hong ,  Merek Siu ,  Arvin Emadi ,  Craig Ciesla

IPC: G01N30/74 ,  C12Q1/6874

Abstract: An apparatus includes a flow cell body, a plurality of electrodes, an imaging assembly, and one or more barrier features. The flow cell body defines one or more flow channels and a plurality of wells defined as recesses in the floor of each flow channel. Each well is fluidically coupled with the corresponding flow channel. The flow cell body further defines interstitial surfaces between adjacent wells. Each well defines a corresponding depth. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are to effect writing of polynucleotides in the wells. The imaging assembly is to capture images of polynucleotides written in the wells. The one or more barrier features are positioned in the wells, between the wells, or above the wells. The one or more barrier features contain reactions in each well, reduce diffusion between the wells, or reduce optical cross-talk between the wells.

公开(公告)号：US20210146356A1

公开(公告)日：2021-05-20

申请号：US17254469

申请日：2020-05-26

Applicant: ILLUMINA, INC.

Inventor： Ali Agah ,  Aathavan Karunakaran ,  Tarun Khurana ,  Stanley Hong ,  Merek Siu ,  Arvin Emadi ,  Craig Ciesla

IPC: B01L3/00

Abstract: An apparatus includes a flow cell body, a plurality of electrodes, an integrated circuit, and an imaging assembly. The flow cell body defines one or more flow channels and a plurality of wells. Each flow channel is configured to receive a flow of fluid. Each well is fluidically coupled with the corresponding flow channel. Each well is configured to contain at least one polynucleotide. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are operable to effect writing of polynucleotides in the corresponding wells. The integrated circuit is operable to drive selective deposition or activation of selected nucleotides to attach to polynucleotides in the wells to thereby generate polynucleotides representing machine-written data in the wells. The imaging assembly is operable to capture images indicative of one or more nucleotides in a polynucleotide.

公开(公告)号：US20210146354A1

公开(公告)日：2021-05-20

申请号：US17254462

申请日：2020-05-26

Applicant: ILLUMINA, INC.

Inventor： Merek Siu ,  Ali Agah ,  Stanley Hong ,  Tarun Khurana ,  Aathavan Karunakaran ,  Craig Ciesla

IPC: B01L3/00

Abstract: Devices, systems, and methods for non-volatile storage include a well activation device operable to modify one or more wells from a plurality of wells of a flow cell to provide a set of readable wells. Readable wells are configured to allow exposure of a well to substances from nucleotide sequencing fluids, and prevent exposure to other substances and fluids, such as nucleotide synthesizing fluids. The well activation device may also modify wells to provide a set of writeable wells. This set of wells is configured to allow exposure to the nucleotide synthesizing fluids and substances; and prevent exposure to the nucleotide sequencing fluids and substances. There may also be provisions made for risk mitigation for data errors such as generating commands to write specified data to a nucleotide sequence associated with a particular location in a storage device, reading the nucleotide sequence and performing a comparison.

公开(公告)号：US20210116436A1

公开(公告)日：2021-04-22

申请号：US17254473

申请日：2020-05-26

Applicant: ILLUMINA, INC.

Inventor： Tarun Khurana ,  Ali Agah ,  Aathavan Karunakaran ,  Xi-Jun Chen

IPC: G01N33/483 ,  G01N33/50 ,  G01N33/53

Abstract: Methods are used for obtaining, cataloguing, and/or storing data derived from a biological source using a flow cell body, electrodes, and an imaging assembly. The data may include DNA and/or RNA obtained from a biological source, such as from the cells of an organism. The methods may be used to obtain, catalog, and/or store data such as DNA or RNA sequence from a pathogen such as a virus and/or a bacteria, human health data over time, and immune system information from an individual. The data obtained using the disclosed methods may be used for a variety of different purposes, including the manufacture of vaccine compositions, and for restoring the immune system of an individual who has undergone an immune system depleting event. The methods may be used for storage of biological cells, which may be used for the screening of compounds, such as small molecules with potential for therapeutic indications.