公开(公告)号：US20250096050A1

公开(公告)日：2025-03-20

申请号：US18971047

申请日：2024-12-06

Applicant: Illumina, Inc.

Inventor： Arvin Emadi ,  Arnaud Rival ,  Ali Agah ,  Tara Bozorg-Grayeli

IPC: H01L23/053 ,  B81B1/00 ,  G01N21/64 ,  H01L23/00 ,  H01L23/15 ,  H01L23/31 ,  H01L23/48

Abstract: Provided herein include various examples of an apparatus, a sensor system and examples of a method for manufacturing aspects of an apparatus, a sensor system. The apparatus may include a die. The apparatus may also include a substrate comprising a cavity. The die may be oriented in a portion of the cavity in the substrate, where the orientation defines a first space in the cavity adjacent to a first edge of the upper surface of the die and a second space in the cavity adjacent to the second edge of the upper surface of the die. The apparatus may further include fluidics fan-out regions comprising a first cured material deposited in the first space and the second space, a surface of the fluidics fan-out regions being contiguous with the upper surface of the die.

公开(公告)号：US12100723B2

公开(公告)日：2024-09-24

申请号：US17418525

申请日：2020-09-29

Applicant: ILLUMINA, INC.

Inventor： Arvin Emadi ,  Ali Agah ,  Xiuyu Cai ,  Craig Lee Hetherington

IPC: H01L27/146

CPC classification number: H01L27/14625 ,  H01L27/14621 ,  H01L27/14623 ,  H01L27/1463 ,  H01L27/1464 ,  H01L27/14685

Abstract: An image sensor structure including an image stack disposed over a device stack. The image stack includes a plurality of light detectors. A first optical filter stack is disposed over the image stack. The first optical filter stack includes a light guide layer. Light pipe cavities are disposed in the light guide layer. Each light pipe cavity is associated with a light detector. Each light pipe cavity has an aspect ratio that is greater than about 2.5 to about 1. A nanowell layer is disposed over the first optical filter stack. Nanowells are disposed in the nanowell layer. Each nanowell is associated with a light detector.

公开(公告)号：US20240145505A1

公开(公告)日：2024-05-02

申请号：US18408992

申请日：2024-01-10

Applicant: Illumina, Inc.

Inventor： Arvin Emadi ,  Arnaud Rival ,  Ali Agah

IPC: H01L27/146 ,  H01L23/00

CPC classification number: H01L27/14618 ,  H01L24/05 ,  H01L27/14636 ,  H01L27/14643 ,  H01L27/14689 ,  H01L27/14698

Abstract: Disclosed in one example is an apparatus including a substrate, a sensor over the substrate including an active surface and a sensor bond pad, a molding layer over the substrate and covering sides of the sensor, the molding layer having a molding height relative to a top surface of the substrate that is greater than a height of the active surface of the sensor relative to the top surface of the substrate, and a lidding layer over the molding layer and over the active surface. The lidding layer and the molding layer form a space over the active surface of the sensor that defines a flow channel.

公开(公告)号：US11867672B2

公开(公告)日：2024-01-09

申请号：US18107752

申请日：2023-02-09

Applicant: ILLUMINA, INC.

Inventor： Tarun Khurana ,  Ali Agah ,  Aathavan Karunakaran ,  Stanley Hong ,  Merek Siu ,  Arvin Emadi ,  Craig Ciesla

IPC: G01N30/74 ,  C12Q1/6874

CPC classification number: G01N30/74 ,  C12Q1/6874 ,  G01N2030/746

Abstract: An apparatus includes a flow cell body, a plurality of electrodes, an imaging assembly, and one or more barrier features. The flow cell body defines one or more flow channels and a plurality of wells defined as recesses in the floor of each flow channel. Each well is fluidically coupled with the corresponding flow channel. The flow cell body further defines interstitial surfaces between adjacent wells. Each well defines a corresponding depth. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are to effect writing of polynucleotides in the wells. The imaging assembly is to capture images of polynucleotides written in the wells. The one or more barrier features are positioned in the wells, between the wells, or above the wells. The one or more barrier features contain reactions in each well, reduce diffusion between the wells, or reduce optical cross-talk between the wells.

公开(公告)号：US20230184725A1

公开(公告)日：2023-06-15

申请号：US18107752

申请日：2023-02-09

Applicant: ILLUMINA, INC.

Inventor： Tarun Khurana ,  Ali Agah ,  Aathavan Karunakaran ,  Stanley Hong ,  Merek Siu ,  Arvin Emadi ,  Craig Ciesla

IPC: G01N30/74 ,  C12Q1/6874

CPC classification number: G01N30/74 ,  C12Q1/6874 ,  G01N2030/746

Abstract: An apparatus includes a flow cell body, a plurality of electrodes, an imaging assembly, and one or more barrier features. The flow cell body defines one or more flow channels and a plurality of wells defined as recesses in the floor of each flow channel. Each well is fluidically coupled with the corresponding flow channel. The flow cell body further defines interstitial surfaces between adjacent wells. Each well defines a corresponding depth. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are to effect writing of polynucleotides in the wells. The imaging assembly is to capture images of polynucleotides written in the wells. The one or more barrier features are positioned in the wells, between the wells, or above the wells. The one or more barrier features contain reactions in each well, reduce diffusion between the wells, or reduce optical cross-talk between the wells.

公开(公告)号：US11604175B2

公开(公告)日：2023-03-14

申请号：US17846538

申请日：2022-06-22

Applicant: ILLUMINA, INC.

Inventor： Tarun Khurana ,  Ali Agah ,  Aathavan Karunakaran ,  Stanley Hong ,  Merek Siu ,  Arvin Emadi ,  Craig Ciesla

IPC: G01N30/74 ,  C12Q1/6874

Abstract: An apparatus includes a flow cell body, a plurality of electrodes, an imaging assembly, and one or more barrier features. The flow cell body defines one or more flow channels and a plurality of wells defined as recesses in the floor of each flow channel. Each well is fluidically coupled with the corresponding flow channel. The flow cell body further defines interstitial surfaces between adjacent wells. Each well defines a corresponding depth. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are to effect writing of polynucleotides in the wells. The imaging assembly is to capture images of polynucleotides written in the wells. The one or more barrier features are positioned in the wells, between the wells, or above the wells. The one or more barrier features contain reactions in each well, reduce diffusion between the wells, or reduce optical cross-talk between the wells.

公开(公告)号：US20230076689A1

公开(公告)日：2023-03-09

申请号：US17896176

申请日：2022-08-26

Applicant: ILLUMINA, INC.

Inventor： Mohsen Rezaei ,  Craig Hetherington ,  Arvin Emadi ,  Stanley Hong

IPC: G01N15/14 ,  G02B5/20 ,  H01L27/146

Abstract: An apparatus includes a flow cell body with an array of reaction sites positioned along a floor of a channel. An optical filter layer is positioned under the floor of the channel and includes at least a portion spanning uninterruptedly along a length corresponding to the length of the array of reaction sites. Imaging regions are positioned under the optical filter layer. Each imaging region is positioned directly under a corresponding reaction site. The optical filter layer is configured to permit one or more selected wavelengths of light to pass from each reaction site to the imaging region forming a sensing pair with the reaction site. The optical filter layer is configured to reduce transmission of excitation light directed toward the reaction sites; and to reduce transmission of light emitted from each reaction site to imaging regions not forming a sensing pair with the reaction site.

公开(公告)号：US20220367547A1

公开(公告)日：2022-11-17

申请号：US17804023

申请日：2022-05-25

Applicant: Illumina, Inc.

Inventor： Arvin Emadi ,  Arnaud Rival ,  Ali Agah

IPC: H01L27/146 ,  H01L23/00

Abstract: Disclosed in one example is an apparatus including a substrate, a sensor over the substrate including an active surface and a sensor bond pad, a molding layer over the substrate and covering sides of the sensor, the molding layer having a molding height relative to a top surface of the substrate that is greater than a height of the active surface of the sensor relative to the top surface of the substrate, and a lidding layer over the molding layer and over the active surface. The lidding layer and the molding layer form a space over the active surface of the sensor that defines a flow channel.

公开(公告)号：US11282588B2

公开(公告)日：2022-03-22

申请号：US17254459

申请日：2020-05-26

Applicant: ILLUMINA, INC.

Inventor： Craig Ciesla ,  Ali Agah ,  Stanley Hong ,  Tarun Khurana ,  Aathavan Karunakaran ,  Arvin Emadi ,  Merek Siu

IPC: G16B50/30 ,  G06F3/06 ,  G16B30/10 ,  C12Q1/6869 ,  G11C13/02 ,  B01L3/00 ,  C12Q1/6874

Abstract: A system writes input data to a storage device as machine-written polynucleotides; and reads machine written polynucleotides from the storage device as output data. The storage device includes a flow cell including a plurality of storage wells in which machine written polynucleotides may be stored. The storage device may include a set of electrodes corresponding to the storage wells that allow for selective interactions with wells across the surface of a flow cell. Operation of the storage device may include receiving a read request associated with a particular location in the storage device, creating a copy of a nucleotide sequence located at the particular location in the storage device, transferring the copy of the nucleotide sequence to a read location, and reading the copy of the nucleotide sequence at the read location.

公开(公告)号：US20230321651A1

公开(公告)日：2023-10-12

申请号：US18202461

申请日：2023-05-26

Applicant: ILLUMINA, INC.

Inventor： Ali Agah ,  Aathavan Karunakaran ,  Tarun Khurana ,  Stanley Hong ,  Merek Siu ,  Arvin Emadi ,  Craig Ciesla

IPC: B01L3/00 ,  G11C13/02 ,  B01J19/00

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L3/502738 ,  G11C13/02 ,  B01J19/0046 ,  C12Q1/6874

Abstract: An apparatus includes a flow cell body, a plurality of electrodes, an integrated circuit, and an imaging assembly. The flow cell body defines one or more flow channels and a plurality of wells. Each flow channel is configured to receive a flow of fluid. Each well is fluidically coupled with the corresponding flow channel. Each well is configured to contain at least one polynucleotide. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are operable to effect writing of polynucleotides in the corresponding wells. The integrated circuit is operable to drive selective deposition or activation of selected nucleotides to attach to polynucleotides in the wells to thereby generate polynucleotides representing machine-written data in the wells. The imaging assembly is operable to capture images indicative of one or more nucleotides in a polynucleotide.