公开(公告)号：US5888739A

公开(公告)日：1999-03-30

申请号：US926054

申请日：1997-09-09

申请人： J. Bruce Pitner ,  James G. Nadeau ,  Glenn P. Vonk

发明人： J. Bruce Pitner ,  James G. Nadeau ,  Glenn P. Vonk

IPC分类号： G01N33/58 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/78

CPC分类号： C12Q1/6818 ,  Y10S436/80 ,  Y10S436/805 ,  Y10T436/143333

摘要： G-quartet structures have been found to be useful in fluorescence assays to detect a selected nucleic acid sequence. When oligonucleotides containing these structures are labeled with a donor fluorophore and an acceptor dye, the folding or interaction of the oligonucleotides in the G-quartet structure brings the donor-acceptor pair into close proximity, allowing an interaction between the two labels which results in quenching of donor fluorescence or a change in other fluorescence properties which are the result of the interaction of two dyes in close proximity. The G-quartet structure unfolds or is otherwise disrupted upon hybridization to its complementary sequence, increasing the distance between the two dye labels. This results in decreased donor quenching or a change in another proximity-related fluorescence parameter. The associated increase in donor fluorescence intensity or the change in another fluorescence parameter may be monitored as an indication of the presence of a selected nucleic acid sequence. Alternatively, in some cases a decrease in acceptor fluorescence may be monitored as an indication of the presence of the selected nucleic acid sequence when the acceptor is also a fluorophore. Related structures, such as the i-tetraplex, may also be useful in similar methods for detection of a selected nucleic acid sequence.

摘要翻译： 已经发现G-四重结构在荧光测定中可用于检测选定的核酸序列。 当含有这些结构的寡核苷酸用供体荧光团和受体染料标记时，G-四重结构中寡核苷酸的折叠或相互作用使供体 - 受体对紧密接近，允许两个标记之间的相互作用导致淬灭 的供体荧光或其他荧光性质的变化，这两种染料是两种染料相互作用的结果。 G-四重结构在与其互补序列杂交时展开或以其它方式被破坏，增加了两个染料标记之间的距离。 这导致供体猝灭减少或另一邻近相关荧光参数的变化。 可以监测供体荧光强度的相关增加或另一荧光参数的变化作为选择的核酸序列的存在的指示。 或者，在一些情况下，当受体也是荧光团时，可以监测受体荧光的降低作为选择的核酸序列的存在的指示。 相关结构，例如i-tetraplex，也可用于检测所选择的核酸序列的相似方法中。

公开(公告)号：US5846726A

公开(公告)日：1998-12-08

申请号：US855085

申请日：1997-05-13

申请人： James G. Nadeau ,  J. Bruce Pitner ,  James L. Schram ,  C. Preston Linn ,  Glenn P. Vonk ,  G. Terrance Walker

发明人： James G. Nadeau ,  J. Bruce Pitner ,  James L. Schram ,  C. Preston Linn ,  Glenn P. Vonk ,  G. Terrance Walker

IPC分类号： G01N21/64 ,  C07H21/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/58 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/6818 ,  C12Q1/6825

摘要： Single-stranded signal primers are modified by linkage to two dyes which form a donor/acceptor dye pair. The two dyes are positioned in sufficiently close spatial proximity on the signal primer that the fluorescence of the first dye is quenched by the second dye. The signal primer may further comprise a restriction endonuclease recognition site (RERS) between the two dyes. As the signal primer is initially single-stranded and remains single-stranded in the absence of target, the restriction endonuclease recognition site is not cleavable or nickable by the restriction endonuclease. In the presence of target, however, signal primer and the restriction endonuclease recognition site are rendered double-stranded and cleavable or nickable by the restriction endonuclease. Cleavage or nicking separates the two dyes and a change in fluorescence due to decreased quenching is detected as an indication of the presence of the target sequence or of target sequence amplification.

公开(公告)号：US5650275A

公开(公告)日：1997-07-22

申请号：US276271

申请日：1994-07-18

申请人： J. Bruce Pitner ,  Douglas P. Malinowski ,  Glenn P. Vonk ,  Larry Gold

发明人： J. Bruce Pitner ,  Douglas P. Malinowski ,  Glenn P. Vonk ,  Larry Gold

IPC分类号： C12Q1/68

CPC分类号： C12Q1/68

摘要： The invention relates to methods of using spectroscopically detectable labeled receptor molecules to determine the presence or absence of a target compound in a sample. In one embodiment, spectroscopically detectable labeled nucleic acid ligands are used to determine the presence or absence of biological targets of interest in biological samples.

摘要翻译： 本发明涉及使用光谱可检测标记的受体分子来确定样品中目标化合物的存在或不存在的方法。 在一个实施方案中，使用光谱可检测的标记的核酸配体来确定生物样品中感兴趣的生物靶标的存在或不存在。

公开(公告)号：US5367058A

公开(公告)日：1994-11-22

申请号：US111699

申请日：1993-08-25

申请人： J. Bruce Pitner ,  Linn, Jr. C. Preston ,  Patrick D. Mize

发明人： J. Bruce Pitner ,  Linn, Jr. C. Preston ,  Patrick D. Mize

IPC分类号： A61K49/16 ,  C07K15/28

CPC分类号： A61K49/16

摘要： The present invention relates to modified antibodies and fragments which display an increased affinity for antigen as compared to the affinity of unmodified antibody for antigen. The most preferred modification is the addition of a thiol group on the surface of the antibody such that a covalent bond forms between the antibody and antigen when they bind to one another.

摘要翻译： 本发明涉及与未修饰的抗体对抗原的亲和力相比显示增加的抗原亲和力的修饰的抗体和片段。 最优选的修饰是在抗体表面上添加硫醇基团，使得当它们彼此结合时，抗体和抗原之间形成共价键。